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Porcine epidemic diarrhea virus: Viral RNA detection and quantification using a validated one-step real time RT-PCR

Since 2014, porcine epidemic diarrhea virus (PEDV) has reemerged in Europe. RT-PCR methods have been described for the detection of PEDV, but none have been validated according to a norm. In this study we described the development and validation of a SYBR™ Green one-step RT-qPCR according to the Fre...

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Detalles Bibliográficos
Autores principales:	Bigault, Lionel, Brown, Paul, Bernard, Cécilia, Blanchard, Yannick, Grasland, Béatrice
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier B.V. 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261358/ https://www.ncbi.nlm.nih.gov/pubmed/32485176 http://dx.doi.org/10.1016/j.jviromet.2020.113906

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