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Characterization of biofilm production in different strains of Acinetobacter baumannii and the effects of chemical compounds on biofilm formation

Acinetobacter baumannii, an important emerging pathogen of nosocomial infections, is known for its ability to form biofilms. Biofilm formation increases the survival rate of A. baumannii on dry surfaces and may contribute to its persistence in the hospital environment, which increases the probabilit...

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Autores principales: Lin, Ming-Feng, Lin, Yun-You, Lan, Chung-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261477/
https://www.ncbi.nlm.nih.gov/pubmed/32523805
http://dx.doi.org/10.7717/peerj.9020
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author Lin, Ming-Feng
Lin, Yun-You
Lan, Chung-Yu
author_facet Lin, Ming-Feng
Lin, Yun-You
Lan, Chung-Yu
author_sort Lin, Ming-Feng
collection PubMed
description Acinetobacter baumannii, an important emerging pathogen of nosocomial infections, is known for its ability to form biofilms. Biofilm formation increases the survival rate of A. baumannii on dry surfaces and may contribute to its persistence in the hospital environment, which increases the probability of nosocomial infections and outbreaks. This study was undertaken to characterize the biofilm production of different strains of A. baumannii and the effects of chemical compounds, especially antibiotics, on biofilm formation. In this study, no statistically significant relationship was observed between the ability to form a biofilm and the antimicrobial susceptibility of the A. baumannii clinical isolates. Biofilm formation caused by A. baumannii ATCC 17978 after gene knockout of two-component regulatory system gene baeR, efflux pump genes emrA/emrB and outer membrane coding gene ompA revealed that all mutant strains had less biofilm formation than the wild-type strain, which was further supported by the images from scanning electron microscopy and confocal laser scanning microscopy. The addition of amikacin, colistin, LL-37 or tannic acid decreased the biofilm formation ability of A. baumannii. In contrast, the addition of lower subinhibitory concentration tigecycline increased the biofilm formation ability of A. baumannii. Minimum biofilm eradication concentrations of amikacin, imipenem, colistin, and tigecycline were increased obviously for both wild type and multidrug resistant clinical strain A. baumannii VGH2. In conclusion, the biofilm formation ability of A. baumannii varied in different strains, involved many genes and could be influenced by many chemical compounds.
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spelling pubmed-72614772020-06-09 Characterization of biofilm production in different strains of Acinetobacter baumannii and the effects of chemical compounds on biofilm formation Lin, Ming-Feng Lin, Yun-You Lan, Chung-Yu PeerJ Microbiology Acinetobacter baumannii, an important emerging pathogen of nosocomial infections, is known for its ability to form biofilms. Biofilm formation increases the survival rate of A. baumannii on dry surfaces and may contribute to its persistence in the hospital environment, which increases the probability of nosocomial infections and outbreaks. This study was undertaken to characterize the biofilm production of different strains of A. baumannii and the effects of chemical compounds, especially antibiotics, on biofilm formation. In this study, no statistically significant relationship was observed between the ability to form a biofilm and the antimicrobial susceptibility of the A. baumannii clinical isolates. Biofilm formation caused by A. baumannii ATCC 17978 after gene knockout of two-component regulatory system gene baeR, efflux pump genes emrA/emrB and outer membrane coding gene ompA revealed that all mutant strains had less biofilm formation than the wild-type strain, which was further supported by the images from scanning electron microscopy and confocal laser scanning microscopy. The addition of amikacin, colistin, LL-37 or tannic acid decreased the biofilm formation ability of A. baumannii. In contrast, the addition of lower subinhibitory concentration tigecycline increased the biofilm formation ability of A. baumannii. Minimum biofilm eradication concentrations of amikacin, imipenem, colistin, and tigecycline were increased obviously for both wild type and multidrug resistant clinical strain A. baumannii VGH2. In conclusion, the biofilm formation ability of A. baumannii varied in different strains, involved many genes and could be influenced by many chemical compounds. PeerJ Inc. 2020-05-28 /pmc/articles/PMC7261477/ /pubmed/32523805 http://dx.doi.org/10.7717/peerj.9020 Text en ©2020 Lin et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Microbiology
Lin, Ming-Feng
Lin, Yun-You
Lan, Chung-Yu
Characterization of biofilm production in different strains of Acinetobacter baumannii and the effects of chemical compounds on biofilm formation
title Characterization of biofilm production in different strains of Acinetobacter baumannii and the effects of chemical compounds on biofilm formation
title_full Characterization of biofilm production in different strains of Acinetobacter baumannii and the effects of chemical compounds on biofilm formation
title_fullStr Characterization of biofilm production in different strains of Acinetobacter baumannii and the effects of chemical compounds on biofilm formation
title_full_unstemmed Characterization of biofilm production in different strains of Acinetobacter baumannii and the effects of chemical compounds on biofilm formation
title_short Characterization of biofilm production in different strains of Acinetobacter baumannii and the effects of chemical compounds on biofilm formation
title_sort characterization of biofilm production in different strains of acinetobacter baumannii and the effects of chemical compounds on biofilm formation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261477/
https://www.ncbi.nlm.nih.gov/pubmed/32523805
http://dx.doi.org/10.7717/peerj.9020
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