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Molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds
Seed priming, a pre-sowing technique that enhances the antioxidant/DNA repair activities during the pre-germinative metabolism, still retains empirical features. We explore for the first time the molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds in orde...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261768/ https://www.ncbi.nlm.nih.gov/pubmed/32528699 http://dx.doi.org/10.1038/s41438-020-0310-8 |
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author | Forti, Chiara Ottobrino, Valentino Bassolino, Laura Toppino, Laura Rotino, Giuseppe Leonardo Pagano, Andrea Macovei, Anca Balestrazzi, Alma |
author_facet | Forti, Chiara Ottobrino, Valentino Bassolino, Laura Toppino, Laura Rotino, Giuseppe Leonardo Pagano, Andrea Macovei, Anca Balestrazzi, Alma |
author_sort | Forti, Chiara |
collection | PubMed |
description | Seed priming, a pre-sowing technique that enhances the antioxidant/DNA repair activities during the pre-germinative metabolism, still retains empirical features. We explore for the first time the molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds in order to identify hallmarks (expression patterns of antioxidant/DNA repair genes combined with free radical profiles) useful to discriminate between high- and low-quality lots. The hydropriming protocol hereby developed anticipated (or even rescued) germination, when applied to lots with variable quality. ROS (reactive oxygen species) raised during hydropriming and dropped after dry-back. Upregulation of antioxidant/DNA repair genes was observed during hydropriming and the subsequent imbibition. Upregulation of SmOGG1 (8-oxoguanine glycosylase/lyase) gene detected in primed seeds at 2 h of imbibition appeared as a promising hallmark. On the basis of these results, the investigation was restricted within the first 2 h of imbibition, to verify whether the molecular landscape was reproducible in different lots. A complex pattern of antioxidant/DNA repair gene expression emerged, reflecting the preponderance of seed lot-specific profiles. Only the low-quality eggplant seeds subjected to hydropriming showed enhanced ROS levels, both in the dry and imbibed state, and this might be a useful signature to discriminate among lots. The plasticity of eggplant pre-germinative metabolism stimulated by priming imposes a plethora of heterogeneous molecular responses that might delay the search for quality hallmarks. However, the information hereby gained could be translated to eggplant wild relatives to speed-up their use in breeding programs or other agronomical applications. |
format | Online Article Text |
id | pubmed-7261768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72617682020-06-10 Molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds Forti, Chiara Ottobrino, Valentino Bassolino, Laura Toppino, Laura Rotino, Giuseppe Leonardo Pagano, Andrea Macovei, Anca Balestrazzi, Alma Hortic Res Article Seed priming, a pre-sowing technique that enhances the antioxidant/DNA repair activities during the pre-germinative metabolism, still retains empirical features. We explore for the first time the molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds in order to identify hallmarks (expression patterns of antioxidant/DNA repair genes combined with free radical profiles) useful to discriminate between high- and low-quality lots. The hydropriming protocol hereby developed anticipated (or even rescued) germination, when applied to lots with variable quality. ROS (reactive oxygen species) raised during hydropriming and dropped after dry-back. Upregulation of antioxidant/DNA repair genes was observed during hydropriming and the subsequent imbibition. Upregulation of SmOGG1 (8-oxoguanine glycosylase/lyase) gene detected in primed seeds at 2 h of imbibition appeared as a promising hallmark. On the basis of these results, the investigation was restricted within the first 2 h of imbibition, to verify whether the molecular landscape was reproducible in different lots. A complex pattern of antioxidant/DNA repair gene expression emerged, reflecting the preponderance of seed lot-specific profiles. Only the low-quality eggplant seeds subjected to hydropriming showed enhanced ROS levels, both in the dry and imbibed state, and this might be a useful signature to discriminate among lots. The plasticity of eggplant pre-germinative metabolism stimulated by priming imposes a plethora of heterogeneous molecular responses that might delay the search for quality hallmarks. However, the information hereby gained could be translated to eggplant wild relatives to speed-up their use in breeding programs or other agronomical applications. Nature Publishing Group UK 2020-06-01 /pmc/articles/PMC7261768/ /pubmed/32528699 http://dx.doi.org/10.1038/s41438-020-0310-8 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Forti, Chiara Ottobrino, Valentino Bassolino, Laura Toppino, Laura Rotino, Giuseppe Leonardo Pagano, Andrea Macovei, Anca Balestrazzi, Alma Molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds |
title | Molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds |
title_full | Molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds |
title_fullStr | Molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds |
title_full_unstemmed | Molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds |
title_short | Molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds |
title_sort | molecular dynamics of pre-germinative metabolism in primed eggplant (solanum melongena l.) seeds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261768/ https://www.ncbi.nlm.nih.gov/pubmed/32528699 http://dx.doi.org/10.1038/s41438-020-0310-8 |
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