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Loading calcium fluorescent probes into protoplasts to detect calcium in the flesh tissue cells of Malus domestica
Cytosolic Ca(2+) plays a key role in signal transduction in plants. Calcium imaging is the most common approach to studying dynamic changes in the cytoplasmic Ca(2+) content. Here, we used mature ‘Fuji’ apples (Malus pumila Mill.) to obtain viable protoplasts from flesh tissue cells by enzymatic hyd...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261807/ https://www.ncbi.nlm.nih.gov/pubmed/32528703 http://dx.doi.org/10.1038/s41438-020-0315-3 |