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Human transcription factor YY1 could upregulate the HIV-1 gene expression
Gene expression in HIV-1 is regulated by the promoters in 5’ long-terminal repeat (LTR) element, which contain multiple DNA regulatory elements that serve as binding sites for cellular transcription factors. YY1 could repress HIV-1 gene expression and latent infection. Here, however, we observed tha...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Biochemistry and Molecular Biology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262509/ https://www.ncbi.nlm.nih.gov/pubmed/31818358 http://dx.doi.org/10.5483/BMBRep.2020.53.5.222 |
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author | Yu, Kyung Lee Jung, Yu Mi Park, Seong Hyun Lee, Seong Deok You, Ji Chang |
author_facet | Yu, Kyung Lee Jung, Yu Mi Park, Seong Hyun Lee, Seong Deok You, Ji Chang |
author_sort | Yu, Kyung Lee |
collection | PubMed |
description | Gene expression in HIV-1 is regulated by the promoters in 5’ long-terminal repeat (LTR) element, which contain multiple DNA regulatory elements that serve as binding sites for cellular transcription factors. YY1 could repress HIV-1 gene expression and latent infection. Here, however, we observed that virus production can be increased by YY1 over-expression and decreased under YY1 depleted condition by siRNA treatment. To identify functional domain(s) of YY1 activation, we constructed a number of YY1 truncated mutants. Our data show that full-length YY1 enhances the viral transcription both through U3 and U3RU5 promoters. Moreover, the C-terminal region (296-414 residues) of YY1 is responsible for the transcriptional upregulation, which could be enhanced further in the presence of the viral Tat protein. The central domain of YY1 (155-295 residues) does not affect LTR activity but has a negative effect on HIV-1 gene expression. Taken together, our study shows that YY1 could act as a transcriptional activator in HIV-1 replication, at least in the early stages of infection. |
format | Online Article Text |
id | pubmed-7262509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Korean Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-72625092020-06-10 Human transcription factor YY1 could upregulate the HIV-1 gene expression Yu, Kyung Lee Jung, Yu Mi Park, Seong Hyun Lee, Seong Deok You, Ji Chang BMB Rep Article Gene expression in HIV-1 is regulated by the promoters in 5’ long-terminal repeat (LTR) element, which contain multiple DNA regulatory elements that serve as binding sites for cellular transcription factors. YY1 could repress HIV-1 gene expression and latent infection. Here, however, we observed that virus production can be increased by YY1 over-expression and decreased under YY1 depleted condition by siRNA treatment. To identify functional domain(s) of YY1 activation, we constructed a number of YY1 truncated mutants. Our data show that full-length YY1 enhances the viral transcription both through U3 and U3RU5 promoters. Moreover, the C-terminal region (296-414 residues) of YY1 is responsible for the transcriptional upregulation, which could be enhanced further in the presence of the viral Tat protein. The central domain of YY1 (155-295 residues) does not affect LTR activity but has a negative effect on HIV-1 gene expression. Taken together, our study shows that YY1 could act as a transcriptional activator in HIV-1 replication, at least in the early stages of infection. Korean Society for Biochemistry and Molecular Biology 2020-05-31 2020-05-31 /pmc/articles/PMC7262509/ /pubmed/31818358 http://dx.doi.org/10.5483/BMBRep.2020.53.5.222 Text en Copyright © 2020 by the The Korean Society for Biochemistry and Molecular Biology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Yu, Kyung Lee Jung, Yu Mi Park, Seong Hyun Lee, Seong Deok You, Ji Chang Human transcription factor YY1 could upregulate the HIV-1 gene expression |
title | Human transcription factor YY1 could upregulate the HIV-1 gene expression |
title_full | Human transcription factor YY1 could upregulate the HIV-1 gene expression |
title_fullStr | Human transcription factor YY1 could upregulate the HIV-1 gene expression |
title_full_unstemmed | Human transcription factor YY1 could upregulate the HIV-1 gene expression |
title_short | Human transcription factor YY1 could upregulate the HIV-1 gene expression |
title_sort | human transcription factor yy1 could upregulate the hiv-1 gene expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262509/ https://www.ncbi.nlm.nih.gov/pubmed/31818358 http://dx.doi.org/10.5483/BMBRep.2020.53.5.222 |
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