Gentamycin-Loaded Bone Cement Released Time against Staphylococcus Aureus by using In Vitro Media as the Strategy for the Patient with Prostethic Joint Infection (PJI) after Hip and Knee Joint Arthroplasty

INTRODUCTION: Joint Arthroplasty is the best treatment modality to improves joint function, relieves pain, and increase the quality of life. There were about 0.5% to 0.8% of patients performed primary total knee and hip arthroplasties has been reported with Prostethic Joint Infection (PJI). Staphylococcus aureus is one of the most virulent bacteria which has identified in the isolation caused by PJI. There were increased number of primary arthroplasties. Gentamycin is an antibiotic which has a bactericidal effect for some gram positive and most aerobic and facultative anaerobic gram negative bacteria. They act by inhibiting protein synthesis. METHOD: This research is an experimental study.The object is Staphylococcus aureus which was treated by Gentamycin-loaded bone cement which were divided into 3 concentrations 5%,10%, and 20%. We have observed the antibacterial effect by measured how was the released time while we put it in agar plates. We controlled them every 2 weeks for observing the inhibition of ring assays which were formed. We were also calculated the diameter of ring assay. RESULT: Drug release profiles showed for 5%,10%,20% Gentamycin-loaded bone cement displayed a long enough drug release time about 14 weeks. The diameters of ring assay didn’t showed if they were affected by the released time. DISCUSSION: We have been observed about Gentamycin-loaded bone cement released time with Staphylococcus aureus,we found if it can exist more than 14 weeks.There have been no study investigated about it before. In the present study by Gaoyang in 2018,they have been observed with vancomycinloaded bone cement,they had found if vancomisin loaded-bone cement provided sufficient effective antibacterial time at least 8 weeks. CONCLUSION: The Gentamycin-loaded bone cement released time against Staphylococcus aureus more than about 14 weeks.There is no different result between the length of released with ring assay diameters.