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miR‐15b enhances the proliferation and migration of lung adenocarcinoma by targeting BCL2

BACKGROUND: Lung adenocarcinoma (LUAD) is a subtype of lung cancer (LC), which is the most common tumor worldwide. Accumulating evidence has elucidated an important role of microRNAs (miRNAs) in mediating the development and progression of several tumors. The purpose of this study was to explore the...

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Autores principales: Wang, Jun, Yao, Shupeng, Diao, Yanping, Geng, Yan, Bi, Yanling, Liu, Guangyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262900/
https://www.ncbi.nlm.nih.gov/pubmed/32220063
http://dx.doi.org/10.1111/1759-7714.13382
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author Wang, Jun
Yao, Shupeng
Diao, Yanping
Geng, Yan
Bi, Yanling
Liu, Guangyue
author_facet Wang, Jun
Yao, Shupeng
Diao, Yanping
Geng, Yan
Bi, Yanling
Liu, Guangyue
author_sort Wang, Jun
collection PubMed
description BACKGROUND: Lung adenocarcinoma (LUAD) is a subtype of lung cancer (LC), which is the most common tumor worldwide. Accumulating evidence has elucidated an important role of microRNAs (miRNAs) in mediating the development and progression of several tumors. The purpose of this study was to explore the role and underlying mechanism of miR‐15b in LUAD. METHODS: CCK‐8 and Transwell assays were conducted to measure the capacities of cell viability and migration in SPC‐A1 cells. Luciferase assay was utilized to verifymiR‐15b direct binding to BCL2 mRNA 3′‐UTR. RESULTS: We determined that miR‐15b was overexpressed in LUAD and miR‐15b overexpression predicted a significantly worse outcome in patients with LUAD. miR‐15b improved LUAD growth in vitro and vivo. miR‐15b enhanced cell migration and epithelial–mesenchymal transition (EMT) in LUAD. miR‐15b promoted cell viability, migration and EMT through inhibiting BCL2 expression by targeting to its mRNA 3′‐UTR. BCL2 reversed functions of miR‐15b on promoting cell proliferation, migration and EMT in SPC‐A1 cells. CONCLUSIONS: miR‐15b promoted cell viability, migration and EMT by targeting BCL2 in LUAD. The newly identified miR‐15b/BCL2 axis provides a novel insight into the pathogenesis of LUAD.
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spelling pubmed-72629002020-06-03 miR‐15b enhances the proliferation and migration of lung adenocarcinoma by targeting BCL2 Wang, Jun Yao, Shupeng Diao, Yanping Geng, Yan Bi, Yanling Liu, Guangyue Thorac Cancer Original Articles BACKGROUND: Lung adenocarcinoma (LUAD) is a subtype of lung cancer (LC), which is the most common tumor worldwide. Accumulating evidence has elucidated an important role of microRNAs (miRNAs) in mediating the development and progression of several tumors. The purpose of this study was to explore the role and underlying mechanism of miR‐15b in LUAD. METHODS: CCK‐8 and Transwell assays were conducted to measure the capacities of cell viability and migration in SPC‐A1 cells. Luciferase assay was utilized to verifymiR‐15b direct binding to BCL2 mRNA 3′‐UTR. RESULTS: We determined that miR‐15b was overexpressed in LUAD and miR‐15b overexpression predicted a significantly worse outcome in patients with LUAD. miR‐15b improved LUAD growth in vitro and vivo. miR‐15b enhanced cell migration and epithelial–mesenchymal transition (EMT) in LUAD. miR‐15b promoted cell viability, migration and EMT through inhibiting BCL2 expression by targeting to its mRNA 3′‐UTR. BCL2 reversed functions of miR‐15b on promoting cell proliferation, migration and EMT in SPC‐A1 cells. CONCLUSIONS: miR‐15b promoted cell viability, migration and EMT by targeting BCL2 in LUAD. The newly identified miR‐15b/BCL2 axis provides a novel insight into the pathogenesis of LUAD. John Wiley & Sons Australia, Ltd 2020-03-27 2020-06 /pmc/articles/PMC7262900/ /pubmed/32220063 http://dx.doi.org/10.1111/1759-7714.13382 Text en © 2020 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Wang, Jun
Yao, Shupeng
Diao, Yanping
Geng, Yan
Bi, Yanling
Liu, Guangyue
miR‐15b enhances the proliferation and migration of lung adenocarcinoma by targeting BCL2
title miR‐15b enhances the proliferation and migration of lung adenocarcinoma by targeting BCL2
title_full miR‐15b enhances the proliferation and migration of lung adenocarcinoma by targeting BCL2
title_fullStr miR‐15b enhances the proliferation and migration of lung adenocarcinoma by targeting BCL2
title_full_unstemmed miR‐15b enhances the proliferation and migration of lung adenocarcinoma by targeting BCL2
title_short miR‐15b enhances the proliferation and migration of lung adenocarcinoma by targeting BCL2
title_sort mir‐15b enhances the proliferation and migration of lung adenocarcinoma by targeting bcl2
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262900/
https://www.ncbi.nlm.nih.gov/pubmed/32220063
http://dx.doi.org/10.1111/1759-7714.13382
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