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The spatial regulation of condensin activity in chromosome condensation
Condensin mediates chromosome condensation, which is essential for proper chromosome segregation during mitosis. Prior to anaphase of budding yeast, the ribosomal DNA (RDN) condenses to a thin loop that is distinct from the rest of the chromosomes. We provide evidence that the establishment and main...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263143/ https://www.ncbi.nlm.nih.gov/pubmed/32354834 http://dx.doi.org/10.1101/gad.335471.119 |
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author | Lamothe, Rebecca Costantino, Lorenzo Koshland, Douglas E. |
author_facet | Lamothe, Rebecca Costantino, Lorenzo Koshland, Douglas E. |
author_sort | Lamothe, Rebecca |
collection | PubMed |
description | Condensin mediates chromosome condensation, which is essential for proper chromosome segregation during mitosis. Prior to anaphase of budding yeast, the ribosomal DNA (RDN) condenses to a thin loop that is distinct from the rest of the chromosomes. We provide evidence that the establishment and maintenance of this RDN condensation requires the regulation of condensin by Cdc5p (polo) kinase. We show that Cdc5p is recruited to the site of condensin binding in the RDN by cohesin, a complex related to condensin. Cdc5p and cohesin prevent condensin from misfolding the RDN into an irreversibly decondensed state. From these and other observations, we propose that the spatial regulation of Cdc5p by cohesin modulates condensin activity to ensure proper RDN folding into a thin loop. This mechanism may be evolutionarily conserved, promoting the thinly condensed constrictions that occur at centromeres and RDN of mitotic chromosomes in plants and animals. |
format | Online Article Text |
id | pubmed-7263143 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-72631432020-12-01 The spatial regulation of condensin activity in chromosome condensation Lamothe, Rebecca Costantino, Lorenzo Koshland, Douglas E. Genes Dev Research Paper Condensin mediates chromosome condensation, which is essential for proper chromosome segregation during mitosis. Prior to anaphase of budding yeast, the ribosomal DNA (RDN) condenses to a thin loop that is distinct from the rest of the chromosomes. We provide evidence that the establishment and maintenance of this RDN condensation requires the regulation of condensin by Cdc5p (polo) kinase. We show that Cdc5p is recruited to the site of condensin binding in the RDN by cohesin, a complex related to condensin. Cdc5p and cohesin prevent condensin from misfolding the RDN into an irreversibly decondensed state. From these and other observations, we propose that the spatial regulation of Cdc5p by cohesin modulates condensin activity to ensure proper RDN folding into a thin loop. This mechanism may be evolutionarily conserved, promoting the thinly condensed constrictions that occur at centromeres and RDN of mitotic chromosomes in plants and animals. Cold Spring Harbor Laboratory Press 2020-06-01 /pmc/articles/PMC7263143/ /pubmed/32354834 http://dx.doi.org/10.1101/gad.335471.119 Text en © 2020 Lamothe et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Paper Lamothe, Rebecca Costantino, Lorenzo Koshland, Douglas E. The spatial regulation of condensin activity in chromosome condensation |
title | The spatial regulation of condensin activity in chromosome condensation |
title_full | The spatial regulation of condensin activity in chromosome condensation |
title_fullStr | The spatial regulation of condensin activity in chromosome condensation |
title_full_unstemmed | The spatial regulation of condensin activity in chromosome condensation |
title_short | The spatial regulation of condensin activity in chromosome condensation |
title_sort | spatial regulation of condensin activity in chromosome condensation |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263143/ https://www.ncbi.nlm.nih.gov/pubmed/32354834 http://dx.doi.org/10.1101/gad.335471.119 |
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