Cargando…
Exosomal Small RNA Sequencing Uncovers Dose-Specific MiRNA Markers for Ionizing Radiation Exposure
INTRODUCTION: Acute exposure to ionizing radiation (IR) is hazardous or even lethal. Accurate estimation of the doses of IR exposure is critical to wisely determining the following treatments. Exosomes are nanoscale vesicles harboring biomolecules and mediate the communications among cells and tissu...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263154/ https://www.ncbi.nlm.nih.gov/pubmed/32528236 http://dx.doi.org/10.1177/1559325820926735 |
_version_ | 1783540753312514048 |
---|---|
author | Zhang, Ying Liu, Jiabin Zhou, Liang Hao, Shuai Ding, Zhenhua Xiao, Lin Zhou, Meijuan |
author_facet | Zhang, Ying Liu, Jiabin Zhou, Liang Hao, Shuai Ding, Zhenhua Xiao, Lin Zhou, Meijuan |
author_sort | Zhang, Ying |
collection | PubMed |
description | INTRODUCTION: Acute exposure to ionizing radiation (IR) is hazardous or even lethal. Accurate estimation of the doses of IR exposure is critical to wisely determining the following treatments. Exosomes are nanoscale vesicles harboring biomolecules and mediate the communications among cells and tissues to influence biological processes. Screening out the microRNAs (miRNAs) contained in exosomes as biomarkers can be useful for estimating the IR exposure doses and exploring the correlation between these miRNAs and the occurrence of disease. METHODS: We treated mice with 2.0, 6.5, and 8.0 Gy doses of IR and collected the mice sera at 0, 24, 48, and 72 hours after exposure. Then, the serum exosomes were isolated by ultracentrifuge and the small RNA portion was extracted for sequencing and the following bioinformatics analysis. Qualitative polymerase chain reaction was performed to validate the potential dose-specific markers. RESULTS: Fifty-six miRNAs (31 upregulated, 25 downregulated) were differentially expressed after exposure of the above 3 IR doses and may act as common IR exposure miRNA markers. Bioinformatic analysis also identified several dosage-specific responsive miRNAs. Importantly, IR-induced miR-151-3p and miR-128-3p were significantly and stably increased at 24 hours in different mouse strains with distinct genetic background after exposed to 8.0 Gy of IR. CONCLUSION: Our study shows that miR-151-3p and miR-128-3p can be used as dose-specific biomarkers of 8.0 Gy IR exposure, which can be used to determine the exposure dose by detecting the amount of the 2 miRNAs in serum exosomes. |
format | Online Article Text |
id | pubmed-7263154 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-72631542020-06-10 Exosomal Small RNA Sequencing Uncovers Dose-Specific MiRNA Markers for Ionizing Radiation Exposure Zhang, Ying Liu, Jiabin Zhou, Liang Hao, Shuai Ding, Zhenhua Xiao, Lin Zhou, Meijuan Dose Response Potential Biomarkers of Radiation Damage INTRODUCTION: Acute exposure to ionizing radiation (IR) is hazardous or even lethal. Accurate estimation of the doses of IR exposure is critical to wisely determining the following treatments. Exosomes are nanoscale vesicles harboring biomolecules and mediate the communications among cells and tissues to influence biological processes. Screening out the microRNAs (miRNAs) contained in exosomes as biomarkers can be useful for estimating the IR exposure doses and exploring the correlation between these miRNAs and the occurrence of disease. METHODS: We treated mice with 2.0, 6.5, and 8.0 Gy doses of IR and collected the mice sera at 0, 24, 48, and 72 hours after exposure. Then, the serum exosomes were isolated by ultracentrifuge and the small RNA portion was extracted for sequencing and the following bioinformatics analysis. Qualitative polymerase chain reaction was performed to validate the potential dose-specific markers. RESULTS: Fifty-six miRNAs (31 upregulated, 25 downregulated) were differentially expressed after exposure of the above 3 IR doses and may act as common IR exposure miRNA markers. Bioinformatic analysis also identified several dosage-specific responsive miRNAs. Importantly, IR-induced miR-151-3p and miR-128-3p were significantly and stably increased at 24 hours in different mouse strains with distinct genetic background after exposed to 8.0 Gy of IR. CONCLUSION: Our study shows that miR-151-3p and miR-128-3p can be used as dose-specific biomarkers of 8.0 Gy IR exposure, which can be used to determine the exposure dose by detecting the amount of the 2 miRNAs in serum exosomes. SAGE Publications 2020-05-29 /pmc/articles/PMC7263154/ /pubmed/32528236 http://dx.doi.org/10.1177/1559325820926735 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Potential Biomarkers of Radiation Damage Zhang, Ying Liu, Jiabin Zhou, Liang Hao, Shuai Ding, Zhenhua Xiao, Lin Zhou, Meijuan Exosomal Small RNA Sequencing Uncovers Dose-Specific MiRNA Markers for Ionizing Radiation Exposure |
title | Exosomal Small RNA Sequencing Uncovers Dose-Specific MiRNA Markers for Ionizing Radiation Exposure |
title_full | Exosomal Small RNA Sequencing Uncovers Dose-Specific MiRNA Markers for Ionizing Radiation Exposure |
title_fullStr | Exosomal Small RNA Sequencing Uncovers Dose-Specific MiRNA Markers for Ionizing Radiation Exposure |
title_full_unstemmed | Exosomal Small RNA Sequencing Uncovers Dose-Specific MiRNA Markers for Ionizing Radiation Exposure |
title_short | Exosomal Small RNA Sequencing Uncovers Dose-Specific MiRNA Markers for Ionizing Radiation Exposure |
title_sort | exosomal small rna sequencing uncovers dose-specific mirna markers for ionizing radiation exposure |
topic | Potential Biomarkers of Radiation Damage |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263154/ https://www.ncbi.nlm.nih.gov/pubmed/32528236 http://dx.doi.org/10.1177/1559325820926735 |
work_keys_str_mv | AT zhangying exosomalsmallrnasequencinguncoversdosespecificmirnamarkersforionizingradiationexposure AT liujiabin exosomalsmallrnasequencinguncoversdosespecificmirnamarkersforionizingradiationexposure AT zhouliang exosomalsmallrnasequencinguncoversdosespecificmirnamarkersforionizingradiationexposure AT haoshuai exosomalsmallrnasequencinguncoversdosespecificmirnamarkersforionizingradiationexposure AT dingzhenhua exosomalsmallrnasequencinguncoversdosespecificmirnamarkersforionizingradiationexposure AT xiaolin exosomalsmallrnasequencinguncoversdosespecificmirnamarkersforionizingradiationexposure AT zhoumeijuan exosomalsmallrnasequencinguncoversdosespecificmirnamarkersforionizingradiationexposure |