CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs

Genome editing has been revolutionized by the CRISPR-Cas9 system. CRISPR-Cas9 is composed of single-molecular guide RNA (sgRNA) and a proteinaceous Cas9 nuclease, which recognizes a specific target sequence and a protospacer adjacent motif (PAM) sequence and, subsequently, cleaves the targeted DNA s...

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Detalles Bibliográficos
Autores principales: Lee, Ho Joung, Kim, Hyun Ju, Lee, Sang Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263196/
https://www.ncbi.nlm.nih.gov/pubmed/32327447
http://dx.doi.org/10.1101/gr.257493.119

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263196/
https://www.ncbi.nlm.nih.gov/pubmed/32327447
http://dx.doi.org/10.1101/gr.257493.119

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