Cargando…

CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs

Genome editing has been revolutionized by the CRISPR-Cas9 system. CRISPR-Cas9 is composed of single-molecular guide RNA (sgRNA) and a proteinaceous Cas9 nuclease, which recognizes a specific target sequence and a protospacer adjacent motif (PAM) sequence and, subsequently, cleaves the targeted DNA s...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lee, Ho Joung, Kim, Hyun Ju, Lee, Sang Jun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2020
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263196/ https://www.ncbi.nlm.nih.gov/pubmed/32327447 http://dx.doi.org/10.1101/gr.257493.119

Ejemplares similares

Mismatch Intolerance of 5′-Truncated sgRNAs in CRISPR/Cas9 Enables Efficient Microbial Single-Base Genome Editing
por: Lee, Ho Joung, et al.
Publicado: (2021)

Customized optical mapping by CRISPR–Cas9 mediated DNA labeling with multiple sgRNAs
por: Abid, Heba Z, et al.
Publicado: (2020)

Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system
por: Shao, Shipeng, et al.
Publicado: (2016)

Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing
por: Liang, Gang, et al.
Publicado: (2016)

Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
por: Kim, Sojung, et al.
Publicado: (2017)

Establishment of CRISPR/Cas9 Genome-Editing System Based on Dual sgRNAs in Flammulina filiformis
por: Liu, Xiaotian, et al.
Publicado: (2022)

Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
por: Kundert, Kale, et al.
Publicado: (2019)

Tetrazine-Ligated CRISPR sgRNAs for Efficient Genome Editing
por: Chen, Zexiang, et al.
Publicado: (2022)

Effects of sgRNAs, Promoters, and Explants on the Gene Editing Efficiency of the CRISPR/Cas9 System in Chinese Kale
por: Huang, Wenli, et al.
Publicado: (2023)

Therapeutic genome editing of an aberrant splice site in β-thalassemia by CRISPR/Cas9 with multiple sgRNAs
por: Yang, Fei, et al.
Publicado: (2023)

Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency
por: Jang, Da Eun, et al.
Publicado: (2018)

CRISPRscan: designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo
por: Moreno-Mateos, Miguel A., et al.
Publicado: (2015)

ge-CRISPR - An integrated pipeline for the prediction and analysis of sgRNAs genome editing efficiency for CRISPR/Cas system
por: Kaur, Karambir, et al.
Publicado: (2016)

Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system
por: Yuan, Tingting, et al.
Publicado: (2019)

Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency
por: García-Tuñón, Ignacio, et al.
Publicado: (2019)

Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
por: Iwasaki, Roman S., et al.
Publicado: (2020)

One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs
por: Zuo, Erwei, et al.
Publicado: (2017)

Artificial sgRNAs engineered for genome editing with new Cas12b orthologs
por: Teng, Fei, et al.
Publicado: (2019)

A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs
por: Tálas, András, et al.
Publicado: (2021)

5′ capped and 3′ polyA-tailed sgRNAs enhance the efficiency of CRISPR-Cas9 system
por: Mu, Wei, et al.
Publicado: (2018)

CRISPRpred: A flexible and efficient tool for sgRNAs on-target activity prediction in CRISPR/Cas9 systems
por: Rahman, Md. Khaledur, et al.
Publicado: (2017)

Rapid generation of maternal mutants via oocyte transgenic expression of CRISPR-Cas9 and sgRNAs in zebrafish
por: Zhang, Chong, et al.
Publicado: (2021)

Evaluating the cleavage efficacy of CRISPR-Cas9 sgRNAs targeting ineffective regions of Arabidopsis thaliana genome
por: Malik, Afsheen, et al.
Publicado: (2021)

CRISPy-web: An online resource to design sgRNAs for CRISPR applications
por: Blin, Kai, et al.
Publicado: (2016)

sgRNA-PSM: Predict sgRNAs On-Target Activity Based on Position-Specific Mismatch
por: Liu, Bin, et al.
Publicado: (2020)

Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0
por: Blin, Kai, et al.
Publicado: (2020)

CIDP: a multi-functional platform for designing CRISPR sgRNAs
por: Xu, Dong, et al.
Publicado: (2023)

Efficient large fragment deletion in plants: double pairs of sgRNAs are better than dual sgRNAs
por: Zhu, Guoning, et al.
Publicado: (2023)

CRISPR/Cas9 mediated editing of the Quorn fungus Fusarium venenatum A3/5 by transient expression of Cas9 and sgRNAs targeting endogenous marker gene PKS12
por: Wilson, Fiona M., et al.
Publicado: (2021)

Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton
por: Ramadan, Mohamed, et al.
Publicado: (2021)

Cas9 cleavage assay for pre-screening of sgRNAs using nicking triggered isothermal amplification
por: Zhang, Kaixiang, et al.
Publicado: (2016)

CRISPRz: a database of zebrafish validated sgRNAs
por: Varshney, Gaurav K., et al.
Publicado: (2016)

Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs
por: Wang, Xianlong, et al.
Publicado: (2015)

CRISPR/Cas9-mediated tissue-specific knockout and cDNA rescue using sgRNAs that target exon-intron junctions in Drosophila melanogaster
por: Chilian, Madison, et al.
Publicado: (2022)

Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
por: Hegde, Mudra, et al.
Publicado: (2018)

Elimination of Specific miRNAs by Naked 14-nt sgRNAs
por: Takahashi, Masayuki, et al.
Publicado: (2012)

Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers
por: Tang, Heng, et al.
Publicado: (2022)

Simultaneous knockout of multiple LHCF genes using single sgRNAs and engineering of a high‐fidelity Cas9 for precise genome editing in marine algae
por: Sharma, Amit K., et al.
Publicado: (2021)

PAVOOC: designing CRISPR sgRNAs using 3D protein structures and functional domain annotations
por: Schaefer, Moritz, et al.
Publicado: (2019)

CRISPR-surfaceome: An online tool for designing highly efficient sgRNAs targeting cell surface proteins
por: Mei, Hong, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_3lt6vrh92f01dg8u88upei23ek