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Deep immune profiling of COVID-19 patients reveals patient heterogeneity and distinct immunotypes with implications for therapeutic interventions

COVID-19 has become a global pandemic. Immune dysregulation has been implicated, but immune responses remain poorly understood. We analyzed 71 COVID-19 patients compared to recovered and healthy subjects using high dimensional cytometry. Integrated analysis of ~200 immune and >30 clinical feature...

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Autores principales: Mathew, Divij, Giles, Josephine R., Baxter, Amy E., Greenplate, Allison R., Wu, Jennifer E., Alanio, Cécile, Oldridge, Derek A., Kuri-Cervantes, Leticia, Pampena, M. Betina, D’Andrea, Kurt, Manne, Sasikanth, Chen, Zeyu, Huang, Yinghui Jane, Reilly, John P., Weisman, Ariel R, Ittner, Caroline A.G., Kuthuru, Oliva, Dougherty, Jeanette, Nzingha, Kito, Han, Nicholas, Kim, Justin, Pattekar, Ajinkya, Goodwin, Eileen C., Anderson, Elizabeth M., Weirick, Madison E., Gouma, Sigrid, Arevalo, Claudia P., Bolton, Marcus J., Chen, Fang, Lacey, Simon F., Hensley, Scott E., Apostolidis, Sokratis, Huang, Alexander C., Vella, Laura A., Betts, Michael R., Meyer, Nuala J., Wherry, E. John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263500/
https://www.ncbi.nlm.nih.gov/pubmed/32511371
http://dx.doi.org/10.1101/2020.05.20.106401
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author Mathew, Divij
Giles, Josephine R.
Baxter, Amy E.
Greenplate, Allison R.
Wu, Jennifer E.
Alanio, Cécile
Oldridge, Derek A.
Kuri-Cervantes, Leticia
Pampena, M. Betina
D’Andrea, Kurt
Manne, Sasikanth
Chen, Zeyu
Huang, Yinghui Jane
Reilly, John P.
Weisman, Ariel R,
Ittner, Caroline A.G.
Kuthuru, Oliva
Dougherty, Jeanette
Nzingha, Kito
Han, Nicholas
Kim, Justin
Pattekar, Ajinkya
Goodwin, Eileen C.
Anderson, Elizabeth M.
Weirick, Madison E.
Gouma, Sigrid
Arevalo, Claudia P.
Bolton, Marcus J.
Chen, Fang
Lacey, Simon F.
Hensley, Scott E.
Apostolidis, Sokratis
Huang, Alexander C.
Vella, Laura A.
Betts, Michael R.
Meyer, Nuala J.
Wherry, E. John
author_facet Mathew, Divij
Giles, Josephine R.
Baxter, Amy E.
Greenplate, Allison R.
Wu, Jennifer E.
Alanio, Cécile
Oldridge, Derek A.
Kuri-Cervantes, Leticia
Pampena, M. Betina
D’Andrea, Kurt
Manne, Sasikanth
Chen, Zeyu
Huang, Yinghui Jane
Reilly, John P.
Weisman, Ariel R,
Ittner, Caroline A.G.
Kuthuru, Oliva
Dougherty, Jeanette
Nzingha, Kito
Han, Nicholas
Kim, Justin
Pattekar, Ajinkya
Goodwin, Eileen C.
Anderson, Elizabeth M.
Weirick, Madison E.
Gouma, Sigrid
Arevalo, Claudia P.
Bolton, Marcus J.
Chen, Fang
Lacey, Simon F.
Hensley, Scott E.
Apostolidis, Sokratis
Huang, Alexander C.
Vella, Laura A.
Betts, Michael R.
Meyer, Nuala J.
Wherry, E. John
author_sort Mathew, Divij
collection PubMed
description COVID-19 has become a global pandemic. Immune dysregulation has been implicated, but immune responses remain poorly understood. We analyzed 71 COVID-19 patients compared to recovered and healthy subjects using high dimensional cytometry. Integrated analysis of ~200 immune and >30 clinical features revealed activation of T cell and B cell subsets, but only in some patients. A subgroup of patients had T cell activation characteristic of acute viral infection and plasmablast responses could reach >30% of circulating B cells. However, another subgroup had lymphocyte activation comparable to uninfected subjects. Stable versus dynamic immunological signatures were identified and linked to trajectories of disease severity change. These analyses identified three “immunotypes” associated with poor clinical trajectories versus improving health. These immunotypes may have implications for therapeutics and vaccines.
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spelling pubmed-72635002020-06-07 Deep immune profiling of COVID-19 patients reveals patient heterogeneity and distinct immunotypes with implications for therapeutic interventions Mathew, Divij Giles, Josephine R. Baxter, Amy E. Greenplate, Allison R. Wu, Jennifer E. Alanio, Cécile Oldridge, Derek A. Kuri-Cervantes, Leticia Pampena, M. Betina D’Andrea, Kurt Manne, Sasikanth Chen, Zeyu Huang, Yinghui Jane Reilly, John P. Weisman, Ariel R, Ittner, Caroline A.G. Kuthuru, Oliva Dougherty, Jeanette Nzingha, Kito Han, Nicholas Kim, Justin Pattekar, Ajinkya Goodwin, Eileen C. Anderson, Elizabeth M. Weirick, Madison E. Gouma, Sigrid Arevalo, Claudia P. Bolton, Marcus J. Chen, Fang Lacey, Simon F. Hensley, Scott E. Apostolidis, Sokratis Huang, Alexander C. Vella, Laura A. Betts, Michael R. Meyer, Nuala J. Wherry, E. John bioRxiv Article COVID-19 has become a global pandemic. Immune dysregulation has been implicated, but immune responses remain poorly understood. We analyzed 71 COVID-19 patients compared to recovered and healthy subjects using high dimensional cytometry. Integrated analysis of ~200 immune and >30 clinical features revealed activation of T cell and B cell subsets, but only in some patients. A subgroup of patients had T cell activation characteristic of acute viral infection and plasmablast responses could reach >30% of circulating B cells. However, another subgroup had lymphocyte activation comparable to uninfected subjects. Stable versus dynamic immunological signatures were identified and linked to trajectories of disease severity change. These analyses identified three “immunotypes” associated with poor clinical trajectories versus improving health. These immunotypes may have implications for therapeutics and vaccines. Cold Spring Harbor Laboratory 2020-05-23 /pmc/articles/PMC7263500/ /pubmed/32511371 http://dx.doi.org/10.1101/2020.05.20.106401 Text en https://creativecommons.org/licenses/by-nd/4.0/This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, and only so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Mathew, Divij
Giles, Josephine R.
Baxter, Amy E.
Greenplate, Allison R.
Wu, Jennifer E.
Alanio, Cécile
Oldridge, Derek A.
Kuri-Cervantes, Leticia
Pampena, M. Betina
D’Andrea, Kurt
Manne, Sasikanth
Chen, Zeyu
Huang, Yinghui Jane
Reilly, John P.
Weisman, Ariel R,
Ittner, Caroline A.G.
Kuthuru, Oliva
Dougherty, Jeanette
Nzingha, Kito
Han, Nicholas
Kim, Justin
Pattekar, Ajinkya
Goodwin, Eileen C.
Anderson, Elizabeth M.
Weirick, Madison E.
Gouma, Sigrid
Arevalo, Claudia P.
Bolton, Marcus J.
Chen, Fang
Lacey, Simon F.
Hensley, Scott E.
Apostolidis, Sokratis
Huang, Alexander C.
Vella, Laura A.
Betts, Michael R.
Meyer, Nuala J.
Wherry, E. John
Deep immune profiling of COVID-19 patients reveals patient heterogeneity and distinct immunotypes with implications for therapeutic interventions
title Deep immune profiling of COVID-19 patients reveals patient heterogeneity and distinct immunotypes with implications for therapeutic interventions
title_full Deep immune profiling of COVID-19 patients reveals patient heterogeneity and distinct immunotypes with implications for therapeutic interventions
title_fullStr Deep immune profiling of COVID-19 patients reveals patient heterogeneity and distinct immunotypes with implications for therapeutic interventions
title_full_unstemmed Deep immune profiling of COVID-19 patients reveals patient heterogeneity and distinct immunotypes with implications for therapeutic interventions
title_short Deep immune profiling of COVID-19 patients reveals patient heterogeneity and distinct immunotypes with implications for therapeutic interventions
title_sort deep immune profiling of covid-19 patients reveals patient heterogeneity and distinct immunotypes with implications for therapeutic interventions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263500/
https://www.ncbi.nlm.nih.gov/pubmed/32511371
http://dx.doi.org/10.1101/2020.05.20.106401
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