A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation

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Virus neutralization remains the gold standard for determining antibody efficacy. Therefore, a high-throughput assay to measure SARS-CoV-2 neutralizing antibodies is urgently needed for COVID-19 serodiagnosis, convalescent plasma therapy, and vaccine development. Here we report on a fluorescence-bas...

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Autores principales: Muruato, Antonio E., Fontes-Garfias, Camila R., Ren, Ping, Garcia-Blanco, Mariano A., Menachery, Vineet D., Xie, Xuping, Shi, Pei-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263515/
https://www.ncbi.nlm.nih.gov/pubmed/32511386
http://dx.doi.org/10.1101/2020.05.21.109546
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author Muruato, Antonio E.
Fontes-Garfias, Camila R.
Ren, Ping
Garcia-Blanco, Mariano A.
Menachery, Vineet D.
Xie, Xuping
Shi, Pei-Yong
author_facet Muruato, Antonio E.
Fontes-Garfias, Camila R.
Ren, Ping
Garcia-Blanco, Mariano A.
Menachery, Vineet D.
Xie, Xuping
Shi, Pei-Yong
author_sort Muruato, Antonio E.
collection PubMed
description Virus neutralization remains the gold standard for determining antibody efficacy. Therefore, a high-throughput assay to measure SARS-CoV-2 neutralizing antibodies is urgently needed for COVID-19 serodiagnosis, convalescent plasma therapy, and vaccine development. Here we report on a fluorescence-based SARS-CoV-2 neutralization assay that detects SARS-CoV-2 neutralizing antibodies in COVID-19 patient specimens and yields comparable results to plaque reduction neutralizing assay, the gold standard of serological testing. Our approach offers a rapid platform that can be scaled to screen people for antibody protection from COVID-19, a key parameter necessary to safely reopen local communities.
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spelling pubmed-72635152020-06-07 A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation Muruato, Antonio E. Fontes-Garfias, Camila R. Ren, Ping Garcia-Blanco, Mariano A. Menachery, Vineet D. Xie, Xuping Shi, Pei-Yong bioRxiv Article Virus neutralization remains the gold standard for determining antibody efficacy. Therefore, a high-throughput assay to measure SARS-CoV-2 neutralizing antibodies is urgently needed for COVID-19 serodiagnosis, convalescent plasma therapy, and vaccine development. Here we report on a fluorescence-based SARS-CoV-2 neutralization assay that detects SARS-CoV-2 neutralizing antibodies in COVID-19 patient specimens and yields comparable results to plaque reduction neutralizing assay, the gold standard of serological testing. Our approach offers a rapid platform that can be scaled to screen people for antibody protection from COVID-19, a key parameter necessary to safely reopen local communities. Cold Spring Harbor Laboratory 2020-05-22 /pmc/articles/PMC7263515/ /pubmed/32511386 http://dx.doi.org/10.1101/2020.05.21.109546 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/It is made available under a CC-BY-NC-ND 4.0 International license (http://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Article
Muruato, Antonio E.
Fontes-Garfias, Camila R.
Ren, Ping
Garcia-Blanco, Mariano A.
Menachery, Vineet D.
Xie, Xuping
Shi, Pei-Yong
A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation
title A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation
title_full A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation
title_fullStr A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation
title_full_unstemmed A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation
title_short A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation
title_sort high-throughput neutralizing antibody assay for covid-19 diagnosis and vaccine evaluation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263515/
https://www.ncbi.nlm.nih.gov/pubmed/32511386
http://dx.doi.org/10.1101/2020.05.21.109546
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