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Serum proteomics analysis of feline mammary carcinoma based on label-free and PRM techniques

BACKGROUND: Feline mammary carcinoma is the third most common cancer that affects female cats. OBJECTIVES: The purpose of this study was to screen differential serum proteins in feline and clarify the relationship between them and the occurrence of feline mammary carcinoma. METHODS: Chinese pastoral...

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Autores principales: Zheng, Jia-San, Wei, Ren-Yue, Wang, Zheng, Zhu, Ting-Ting, Ruan, Hong-Ri, Wei, Xue, Hou, Kai-Wen, Wu, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263907/
https://www.ncbi.nlm.nih.gov/pubmed/32476319
http://dx.doi.org/10.4142/jvs.2020.21.e45
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author Zheng, Jia-San
Wei, Ren-Yue
Wang, Zheng
Zhu, Ting-Ting
Ruan, Hong-Ri
Wei, Xue
Hou, Kai-Wen
Wu, Rui
author_facet Zheng, Jia-San
Wei, Ren-Yue
Wang, Zheng
Zhu, Ting-Ting
Ruan, Hong-Ri
Wei, Xue
Hou, Kai-Wen
Wu, Rui
author_sort Zheng, Jia-San
collection PubMed
description BACKGROUND: Feline mammary carcinoma is the third most common cancer that affects female cats. OBJECTIVES: The purpose of this study was to screen differential serum proteins in feline and clarify the relationship between them and the occurrence of feline mammary carcinoma. METHODS: Chinese pastoral cats were used as experimental animals. Six serum samples from cats with mammary carcinoma (group T) and six serum samples from healthy cats (group C) were selected. Differential protein analysis was performed using a Label-free technique, while parallel reaction monitoring (PRM) was performed to verify the screened differential proteins. RESULTS: A total of 82 differential proteins were detected between group T and group C, of which 55 proteins were down regulated and 27 proteins were up regulated. Apolipoprotein A-I, Apolipoprotein A-II (ApoA-II), Apolipoprotein B (ApoB), Apolipoprotein C-III (ApoC-III), coagulation factor V, coagulation factor X, C1q, albumen (ALB) were all associated with the occurrence of feline mammary carcinoma. Differential proteins were involved in a total of 40 signaling pathways, among which the metabolic pathways associated with feline mammary carcinoma were the complement and coagulation cascade and cholesterol metabolism. According to the Label-free results, ApoB, ApoC-III, ApoA-II, FN1, an uncharacterized protein, and ALB were selected for PRM target verification. The results were consistent with the trend of the label-free. CONCLUSIONS: This experimen is the first to confirm ApoA-II and ApoB maybe new feline mammary carcinoma biomarkers and to analyze their mechanisms in the development of such carcinoma in feline.
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spelling pubmed-72639072020-06-10 Serum proteomics analysis of feline mammary carcinoma based on label-free and PRM techniques Zheng, Jia-San Wei, Ren-Yue Wang, Zheng Zhu, Ting-Ting Ruan, Hong-Ri Wei, Xue Hou, Kai-Wen Wu, Rui J Vet Sci Original Article BACKGROUND: Feline mammary carcinoma is the third most common cancer that affects female cats. OBJECTIVES: The purpose of this study was to screen differential serum proteins in feline and clarify the relationship between them and the occurrence of feline mammary carcinoma. METHODS: Chinese pastoral cats were used as experimental animals. Six serum samples from cats with mammary carcinoma (group T) and six serum samples from healthy cats (group C) were selected. Differential protein analysis was performed using a Label-free technique, while parallel reaction monitoring (PRM) was performed to verify the screened differential proteins. RESULTS: A total of 82 differential proteins were detected between group T and group C, of which 55 proteins were down regulated and 27 proteins were up regulated. Apolipoprotein A-I, Apolipoprotein A-II (ApoA-II), Apolipoprotein B (ApoB), Apolipoprotein C-III (ApoC-III), coagulation factor V, coagulation factor X, C1q, albumen (ALB) were all associated with the occurrence of feline mammary carcinoma. Differential proteins were involved in a total of 40 signaling pathways, among which the metabolic pathways associated with feline mammary carcinoma were the complement and coagulation cascade and cholesterol metabolism. According to the Label-free results, ApoB, ApoC-III, ApoA-II, FN1, an uncharacterized protein, and ALB were selected for PRM target verification. The results were consistent with the trend of the label-free. CONCLUSIONS: This experimen is the first to confirm ApoA-II and ApoB maybe new feline mammary carcinoma biomarkers and to analyze their mechanisms in the development of such carcinoma in feline. The Korean Society of Veterinary Science 2020-05 2020-04-23 /pmc/articles/PMC7263907/ /pubmed/32476319 http://dx.doi.org/10.4142/jvs.2020.21.e45 Text en © 2020 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zheng, Jia-San
Wei, Ren-Yue
Wang, Zheng
Zhu, Ting-Ting
Ruan, Hong-Ri
Wei, Xue
Hou, Kai-Wen
Wu, Rui
Serum proteomics analysis of feline mammary carcinoma based on label-free and PRM techniques
title Serum proteomics analysis of feline mammary carcinoma based on label-free and PRM techniques
title_full Serum proteomics analysis of feline mammary carcinoma based on label-free and PRM techniques
title_fullStr Serum proteomics analysis of feline mammary carcinoma based on label-free and PRM techniques
title_full_unstemmed Serum proteomics analysis of feline mammary carcinoma based on label-free and PRM techniques
title_short Serum proteomics analysis of feline mammary carcinoma based on label-free and PRM techniques
title_sort serum proteomics analysis of feline mammary carcinoma based on label-free and prm techniques
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263907/
https://www.ncbi.nlm.nih.gov/pubmed/32476319
http://dx.doi.org/10.4142/jvs.2020.21.e45
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