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Selective Cytotoxic Activity of Prodigiosin@halloysite Nanoformulation

Prodigiosin, a bioactive secondary metabolite produced by Serratia marcescens, is an effective proapoptotic agent against various cancer cell lines, with little or no toxicity toward normal cells. The hydrophobicity of prodigiosin limits its use for medical and biotechnological applications, these l...

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Autores principales: Guryanov, Ivan, Naumenko, Ekaterina, Akhatova, Farida, Lazzara, Giuseppe, Cavallaro, Giuseppe, Nigamatzyanova, Läysän, Fakhrullin, Rawil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264093/
https://www.ncbi.nlm.nih.gov/pubmed/32528938
http://dx.doi.org/10.3389/fbioe.2020.00424
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author Guryanov, Ivan
Naumenko, Ekaterina
Akhatova, Farida
Lazzara, Giuseppe
Cavallaro, Giuseppe
Nigamatzyanova, Läysän
Fakhrullin, Rawil
author_facet Guryanov, Ivan
Naumenko, Ekaterina
Akhatova, Farida
Lazzara, Giuseppe
Cavallaro, Giuseppe
Nigamatzyanova, Läysän
Fakhrullin, Rawil
author_sort Guryanov, Ivan
collection PubMed
description Prodigiosin, a bioactive secondary metabolite produced by Serratia marcescens, is an effective proapoptotic agent against various cancer cell lines, with little or no toxicity toward normal cells. The hydrophobicity of prodigiosin limits its use for medical and biotechnological applications, these limitations, however, can be overcome by using nanoscale drug carriers, resulting in promising formulations for target delivery systems with great potential for anticancer therapy. Here we report on prodigiosin-loaded halloysite-based nanoformulation and its effects on viability of malignant and non-malignant cells. We have found that prodigiosin-loaded halloysite nanotubes inhibit human epithelial colorectal adenocarcinoma (Caco-2) and human colon carcinoma (HCT116) cells proliferative activity. After treatment of Caco-2 cells with prodigiosin-loaded halloysite nanotubes, we have observed a disorganization of the F-actin structure. Comparison of this effects on malignant (Caco-2, HCT116) and non-malignant (MSC, HSF) cells suggests the selective cytotoxic and genotoxic activity of prodigiosin-HNTs nanoformulation.
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spelling pubmed-72640932020-06-10 Selective Cytotoxic Activity of Prodigiosin@halloysite Nanoformulation Guryanov, Ivan Naumenko, Ekaterina Akhatova, Farida Lazzara, Giuseppe Cavallaro, Giuseppe Nigamatzyanova, Läysän Fakhrullin, Rawil Front Bioeng Biotechnol Bioengineering and Biotechnology Prodigiosin, a bioactive secondary metabolite produced by Serratia marcescens, is an effective proapoptotic agent against various cancer cell lines, with little or no toxicity toward normal cells. The hydrophobicity of prodigiosin limits its use for medical and biotechnological applications, these limitations, however, can be overcome by using nanoscale drug carriers, resulting in promising formulations for target delivery systems with great potential for anticancer therapy. Here we report on prodigiosin-loaded halloysite-based nanoformulation and its effects on viability of malignant and non-malignant cells. We have found that prodigiosin-loaded halloysite nanotubes inhibit human epithelial colorectal adenocarcinoma (Caco-2) and human colon carcinoma (HCT116) cells proliferative activity. After treatment of Caco-2 cells with prodigiosin-loaded halloysite nanotubes, we have observed a disorganization of the F-actin structure. Comparison of this effects on malignant (Caco-2, HCT116) and non-malignant (MSC, HSF) cells suggests the selective cytotoxic and genotoxic activity of prodigiosin-HNTs nanoformulation. Frontiers Media S.A. 2020-05-26 /pmc/articles/PMC7264093/ /pubmed/32528938 http://dx.doi.org/10.3389/fbioe.2020.00424 Text en Copyright © 2020 Guryanov, Naumenko, Akhatova, Lazzara, Cavallaro, Nigamatzyanova and Fakhrullin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Guryanov, Ivan
Naumenko, Ekaterina
Akhatova, Farida
Lazzara, Giuseppe
Cavallaro, Giuseppe
Nigamatzyanova, Läysän
Fakhrullin, Rawil
Selective Cytotoxic Activity of Prodigiosin@halloysite Nanoformulation
title Selective Cytotoxic Activity of Prodigiosin@halloysite Nanoformulation
title_full Selective Cytotoxic Activity of Prodigiosin@halloysite Nanoformulation
title_fullStr Selective Cytotoxic Activity of Prodigiosin@halloysite Nanoformulation
title_full_unstemmed Selective Cytotoxic Activity of Prodigiosin@halloysite Nanoformulation
title_short Selective Cytotoxic Activity of Prodigiosin@halloysite Nanoformulation
title_sort selective cytotoxic activity of prodigiosin@halloysite nanoformulation
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264093/
https://www.ncbi.nlm.nih.gov/pubmed/32528938
http://dx.doi.org/10.3389/fbioe.2020.00424
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