Cargando…

Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter

Cell sorting via flow cytometry is a powerful tool to select subpopulations of cells in many biological fields. Selection of fertilisation-prone sperm is a critical step to ensure a stable and high fertilisation rate in in vitro fertilisation (IVF). However, a combination of conventional cell sortin...

Descripción completa

Detalles Bibliográficos
Autores principales: Nakao, Satohiro, Takeo, Toru, Watanabe, Hitomi, Kondoh, Gen, Nakagata, Naomi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264210/
https://www.ncbi.nlm.nih.gov/pubmed/32483250
http://dx.doi.org/10.1038/s41598-020-65931-z
_version_ 1783540927791366144
author Nakao, Satohiro
Takeo, Toru
Watanabe, Hitomi
Kondoh, Gen
Nakagata, Naomi
author_facet Nakao, Satohiro
Takeo, Toru
Watanabe, Hitomi
Kondoh, Gen
Nakagata, Naomi
author_sort Nakao, Satohiro
collection PubMed
description Cell sorting via flow cytometry is a powerful tool to select subpopulations of cells in many biological fields. Selection of fertilisation-prone sperm is a critical step to ensure a stable and high fertilisation rate in in vitro fertilisation (IVF). However, a combination of conventional cell sorting and IVF system has not been established because of severe mechanical damages to the sperm during the sorting process. A cell sorter with microfluidics chip technology that lessens cell damage during cell sorting may address this problem. We evaluated the effects of microfluidics chip cell sorting on the sperm using the parameters, such as motility and fertility, and found this cell sorting method had minimal harmful effect on the sperm. Then, sperm were selected by a marker for acrosome reaction and showed higher fertilisation rate than that of the population of acrosome-intact sperm. Embryo derived from these sperm developed normally. These results indicated that microfluidics chip cell sorting can select fertile sperm to improve IVF technique.
format Online
Article
Text
id pubmed-7264210
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-72642102020-06-05 Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter Nakao, Satohiro Takeo, Toru Watanabe, Hitomi Kondoh, Gen Nakagata, Naomi Sci Rep Article Cell sorting via flow cytometry is a powerful tool to select subpopulations of cells in many biological fields. Selection of fertilisation-prone sperm is a critical step to ensure a stable and high fertilisation rate in in vitro fertilisation (IVF). However, a combination of conventional cell sorting and IVF system has not been established because of severe mechanical damages to the sperm during the sorting process. A cell sorter with microfluidics chip technology that lessens cell damage during cell sorting may address this problem. We evaluated the effects of microfluidics chip cell sorting on the sperm using the parameters, such as motility and fertility, and found this cell sorting method had minimal harmful effect on the sperm. Then, sperm were selected by a marker for acrosome reaction and showed higher fertilisation rate than that of the population of acrosome-intact sperm. Embryo derived from these sperm developed normally. These results indicated that microfluidics chip cell sorting can select fertile sperm to improve IVF technique. Nature Publishing Group UK 2020-06-01 /pmc/articles/PMC7264210/ /pubmed/32483250 http://dx.doi.org/10.1038/s41598-020-65931-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nakao, Satohiro
Takeo, Toru
Watanabe, Hitomi
Kondoh, Gen
Nakagata, Naomi
Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter
title Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter
title_full Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter
title_fullStr Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter
title_full_unstemmed Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter
title_short Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter
title_sort successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264210/
https://www.ncbi.nlm.nih.gov/pubmed/32483250
http://dx.doi.org/10.1038/s41598-020-65931-z
work_keys_str_mv AT nakaosatohiro successfulselectionofmousespermwithhighviabilityandfertilityusingmicrofluidicschipcellsorter
AT takeotoru successfulselectionofmousespermwithhighviabilityandfertilityusingmicrofluidicschipcellsorter
AT watanabehitomi successfulselectionofmousespermwithhighviabilityandfertilityusingmicrofluidicschipcellsorter
AT kondohgen successfulselectionofmousespermwithhighviabilityandfertilityusingmicrofluidicschipcellsorter
AT nakagatanaomi successfulselectionofmousespermwithhighviabilityandfertilityusingmicrofluidicschipcellsorter