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Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10

Localized surface plasmon resonance (LSPR) is a powerful platform for detecting biomolecules including proteins, nucleotides, and vesicles. Here, we report a colloidal gold (Au) nanoparticle-based assay that enhances the LSPR signal of nanoimprinted Au strips. The binding of the colloidal Au nanopar...

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Autores principales: Baek, Seung Hee, Song, Hyun Woo, Lee, Sunwoong, Kim, Jung-Eun, Kim, Yeo Hyang, Wi, Jung-Sub, Ok, Jong G., Park, Jun Seok, Hong, Seonki, Kwak, Moon Kyu, Lee, Hye Jin, Nam, Sung-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264386/
https://www.ncbi.nlm.nih.gov/pubmed/32528922
http://dx.doi.org/10.3389/fchem.2020.00285
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author Baek, Seung Hee
Song, Hyun Woo
Lee, Sunwoong
Kim, Jung-Eun
Kim, Yeo Hyang
Wi, Jung-Sub
Ok, Jong G.
Park, Jun Seok
Hong, Seonki
Kwak, Moon Kyu
Lee, Hye Jin
Nam, Sung-Wook
author_facet Baek, Seung Hee
Song, Hyun Woo
Lee, Sunwoong
Kim, Jung-Eun
Kim, Yeo Hyang
Wi, Jung-Sub
Ok, Jong G.
Park, Jun Seok
Hong, Seonki
Kwak, Moon Kyu
Lee, Hye Jin
Nam, Sung-Wook
author_sort Baek, Seung Hee
collection PubMed
description Localized surface plasmon resonance (LSPR) is a powerful platform for detecting biomolecules including proteins, nucleotides, and vesicles. Here, we report a colloidal gold (Au) nanoparticle-based assay that enhances the LSPR signal of nanoimprinted Au strips. The binding of the colloidal Au nanoparticle on the Au strip causes a red-shift of the LSPR extinction peak, enabling the detection of interleukin-10 (IL-10) cytokine. For LSPR sensor fabrication, we employed a roll-to-roll nanoimprinting process to create nanograting structures on polyethylene terephthalate (PET) film. By the angled deposition of Au on the PET film, we demonstrated a double-bent Au structure with a strong LSPR extinction peak at ~760 nm. Using the Au LSPR sensor, we developed an enzyme-linked immunosorbent assay (ELISA) protocol by forming a sandwich structure of IL-10 capture antibody/IL-10/IL-10 detection antibody. To enhance the LSPR signal, we introduced colloidal Au nanocube (AuNC) to be cross-linked with IL-10 detection antibody for immunogold assay. Using IL-10 as a model protein, we successfully achieved nanomolar sensitivity. We confirmed that the shift of the extinction peak was improved by 450% due to plasmon coupling between AuNC and Au strip. We expect that the AuNC-assisted LSPR sensor platform can be utilized as a diagnostic tool by providing convenient and fast detection of the LSPR signal.
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spelling pubmed-72643862020-06-10 Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10 Baek, Seung Hee Song, Hyun Woo Lee, Sunwoong Kim, Jung-Eun Kim, Yeo Hyang Wi, Jung-Sub Ok, Jong G. Park, Jun Seok Hong, Seonki Kwak, Moon Kyu Lee, Hye Jin Nam, Sung-Wook Front Chem Chemistry Localized surface plasmon resonance (LSPR) is a powerful platform for detecting biomolecules including proteins, nucleotides, and vesicles. Here, we report a colloidal gold (Au) nanoparticle-based assay that enhances the LSPR signal of nanoimprinted Au strips. The binding of the colloidal Au nanoparticle on the Au strip causes a red-shift of the LSPR extinction peak, enabling the detection of interleukin-10 (IL-10) cytokine. For LSPR sensor fabrication, we employed a roll-to-roll nanoimprinting process to create nanograting structures on polyethylene terephthalate (PET) film. By the angled deposition of Au on the PET film, we demonstrated a double-bent Au structure with a strong LSPR extinction peak at ~760 nm. Using the Au LSPR sensor, we developed an enzyme-linked immunosorbent assay (ELISA) protocol by forming a sandwich structure of IL-10 capture antibody/IL-10/IL-10 detection antibody. To enhance the LSPR signal, we introduced colloidal Au nanocube (AuNC) to be cross-linked with IL-10 detection antibody for immunogold assay. Using IL-10 as a model protein, we successfully achieved nanomolar sensitivity. We confirmed that the shift of the extinction peak was improved by 450% due to plasmon coupling between AuNC and Au strip. We expect that the AuNC-assisted LSPR sensor platform can be utilized as a diagnostic tool by providing convenient and fast detection of the LSPR signal. Frontiers Media S.A. 2020-05-26 /pmc/articles/PMC7264386/ /pubmed/32528922 http://dx.doi.org/10.3389/fchem.2020.00285 Text en Copyright © 2020 Baek, Song, Lee, Kim, Kim, Wi, Ok, Park, Hong, Kwak, Lee and Nam. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Baek, Seung Hee
Song, Hyun Woo
Lee, Sunwoong
Kim, Jung-Eun
Kim, Yeo Hyang
Wi, Jung-Sub
Ok, Jong G.
Park, Jun Seok
Hong, Seonki
Kwak, Moon Kyu
Lee, Hye Jin
Nam, Sung-Wook
Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10
title Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10
title_full Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10
title_fullStr Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10
title_full_unstemmed Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10
title_short Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10
title_sort gold nanoparticle-enhanced and roll-to-roll nanoimprinted lspr platform for detecting interleukin-10
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264386/
https://www.ncbi.nlm.nih.gov/pubmed/32528922
http://dx.doi.org/10.3389/fchem.2020.00285
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