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A Tractable Drosophila Cell System Enables Rapid Identification of Acinetobacter baumannii Host Factors

Acinetobacter baumannii is an important causative agent of nosocomial infections worldwide. The pathogen also readily acquires resistance to antibiotics, and pan-resistant strains have been reported. A. baumannii is widely regarded as an extracellular bacterial pathogen. However, accumulating eviden...

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Autores principales: Qin, Qing-Ming, Pei, Jianwu, Gomez, Gabriel, Rice-Ficht, Allison, Ficht, Thomas A., de Figueiredo, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264411/
https://www.ncbi.nlm.nih.gov/pubmed/32528902
http://dx.doi.org/10.3389/fcimb.2020.00240
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author Qin, Qing-Ming
Pei, Jianwu
Gomez, Gabriel
Rice-Ficht, Allison
Ficht, Thomas A.
de Figueiredo, Paul
author_facet Qin, Qing-Ming
Pei, Jianwu
Gomez, Gabriel
Rice-Ficht, Allison
Ficht, Thomas A.
de Figueiredo, Paul
author_sort Qin, Qing-Ming
collection PubMed
description Acinetobacter baumannii is an important causative agent of nosocomial infections worldwide. The pathogen also readily acquires resistance to antibiotics, and pan-resistant strains have been reported. A. baumannii is widely regarded as an extracellular bacterial pathogen. However, accumulating evidence demonstrates that the pathogen can invade, survive or persist in infected mammalian cells. Unfortunately, the molecular mechanisms controlling these processes remain poorly understood. Here, we show that Drosophila S2 cells provide several attractive advantages as a model system for investigating the intracellular lifestyle of the pathogen, including susceptibility to bacterial intracellular replication and limited infection-induced host cell death. We also show that the Drosophila system can be used to rapidly identify host factors, including MAP kinase proteins, which confer susceptibility to intracellular parasitism. Finally, analysis of the Drosophila system suggested that host proteins that regulate organelle biogenesis and membrane trafficking contribute to regulating the intracellular lifestyle of the pathogen. Taken together, these findings establish a novel model system for elucidating interactions between A. baumannii and host cells, define new factors that regulate bacterial invasion or intracellular persistence, and identify subcellular compartments in host cells that interact with the pathogen.
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spelling pubmed-72644112020-06-10 A Tractable Drosophila Cell System Enables Rapid Identification of Acinetobacter baumannii Host Factors Qin, Qing-Ming Pei, Jianwu Gomez, Gabriel Rice-Ficht, Allison Ficht, Thomas A. de Figueiredo, Paul Front Cell Infect Microbiol Cellular and Infection Microbiology Acinetobacter baumannii is an important causative agent of nosocomial infections worldwide. The pathogen also readily acquires resistance to antibiotics, and pan-resistant strains have been reported. A. baumannii is widely regarded as an extracellular bacterial pathogen. However, accumulating evidence demonstrates that the pathogen can invade, survive or persist in infected mammalian cells. Unfortunately, the molecular mechanisms controlling these processes remain poorly understood. Here, we show that Drosophila S2 cells provide several attractive advantages as a model system for investigating the intracellular lifestyle of the pathogen, including susceptibility to bacterial intracellular replication and limited infection-induced host cell death. We also show that the Drosophila system can be used to rapidly identify host factors, including MAP kinase proteins, which confer susceptibility to intracellular parasitism. Finally, analysis of the Drosophila system suggested that host proteins that regulate organelle biogenesis and membrane trafficking contribute to regulating the intracellular lifestyle of the pathogen. Taken together, these findings establish a novel model system for elucidating interactions between A. baumannii and host cells, define new factors that regulate bacterial invasion or intracellular persistence, and identify subcellular compartments in host cells that interact with the pathogen. Frontiers Media S.A. 2020-05-26 /pmc/articles/PMC7264411/ /pubmed/32528902 http://dx.doi.org/10.3389/fcimb.2020.00240 Text en Copyright © 2020 Qin, Pei, Gomez, Rice-Ficht, Ficht and de Figueiredo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Qin, Qing-Ming
Pei, Jianwu
Gomez, Gabriel
Rice-Ficht, Allison
Ficht, Thomas A.
de Figueiredo, Paul
A Tractable Drosophila Cell System Enables Rapid Identification of Acinetobacter baumannii Host Factors
title A Tractable Drosophila Cell System Enables Rapid Identification of Acinetobacter baumannii Host Factors
title_full A Tractable Drosophila Cell System Enables Rapid Identification of Acinetobacter baumannii Host Factors
title_fullStr A Tractable Drosophila Cell System Enables Rapid Identification of Acinetobacter baumannii Host Factors
title_full_unstemmed A Tractable Drosophila Cell System Enables Rapid Identification of Acinetobacter baumannii Host Factors
title_short A Tractable Drosophila Cell System Enables Rapid Identification of Acinetobacter baumannii Host Factors
title_sort tractable drosophila cell system enables rapid identification of acinetobacter baumannii host factors
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264411/
https://www.ncbi.nlm.nih.gov/pubmed/32528902
http://dx.doi.org/10.3389/fcimb.2020.00240
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