Cargando…
Deep-Sequencing Identification of MicroRNA Biomarkers in Serum Exosomes for Early Pig Pregnancy
Early pregnancy diagnosis in sows can significantly improve the efficiency of pig industry. Exosomes are membrane-covered nanovesicles that can transport microRNAs (miRNAs) and other molecular signals between cells. In other species, serum exosome-derived miRNAs can serve as good biomarkers of disea...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264423/ https://www.ncbi.nlm.nih.gov/pubmed/32528535 http://dx.doi.org/10.3389/fgene.2020.00536 |
_version_ | 1783540972849725440 |
---|---|
author | Zhou, Chen Cai, Gengyuan Meng, Fanming Xu, Zhiqian He, Yanjuan Hu, Qun Zheng, Enqin Huang, Sixiu Xu, Zheng Gu, Ting Hu, Bin Wu, Zhenfang Hong, Linjun |
author_facet | Zhou, Chen Cai, Gengyuan Meng, Fanming Xu, Zhiqian He, Yanjuan Hu, Qun Zheng, Enqin Huang, Sixiu Xu, Zheng Gu, Ting Hu, Bin Wu, Zhenfang Hong, Linjun |
author_sort | Zhou, Chen |
collection | PubMed |
description | Early pregnancy diagnosis in sows can significantly improve the efficiency of pig industry. Exosomes are membrane-covered nanovesicles that can transport microRNAs (miRNAs) and other molecular signals between cells. In other species, serum exosome-derived miRNAs can serve as good biomarkers of diseases and different physiological states, including pregnancy status. We hypothesized that circulating exosome-derived miRNAs might be used to differentiate the pregnancy status as early as several days after insemination in pigs. To test this hypothesis, we randomly assigned pigs for artificial insemination with fertile or dead semen (control group). Serum samples were obtained from pregnant pigs on days 9, 12, and 15 after insemination and from non-pregnant pigs on days 0, 9, 12, and 15 after insemination. Exosomes were isolated for RNA extraction. The exosomal RNA samples from pigs on day 9 of the estrus cycle and pregnancy were used for small-RNA sequencing. A total 321 miRNAs were identified in all samples. Twenty eight differentially abundant miRNAs were identified between the pregnant and control groups. miRNAs with | log2 (fold change)| > 2 from sequencing results were selected for validation by quantitative reverse-transcription-polymerase chain reaction (RT-qPCR) in larger samples. Finally two upregulated miRNAs (miR-92b-3p and miR-17-5p) in the pregnant groups (on days 9, 12, and 15 of pregnancy) were confirmed by RT-qPCR. In summary, we have successfully identified circulating exosomal miRNA profiles in the serum of pigs in early pregnancy. miR-92b-3p and miR-17-5p could be used as potential circulating biomarkers for early pregnancy diagnosis. |
format | Online Article Text |
id | pubmed-7264423 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72644232020-06-10 Deep-Sequencing Identification of MicroRNA Biomarkers in Serum Exosomes for Early Pig Pregnancy Zhou, Chen Cai, Gengyuan Meng, Fanming Xu, Zhiqian He, Yanjuan Hu, Qun Zheng, Enqin Huang, Sixiu Xu, Zheng Gu, Ting Hu, Bin Wu, Zhenfang Hong, Linjun Front Genet Genetics Early pregnancy diagnosis in sows can significantly improve the efficiency of pig industry. Exosomes are membrane-covered nanovesicles that can transport microRNAs (miRNAs) and other molecular signals between cells. In other species, serum exosome-derived miRNAs can serve as good biomarkers of diseases and different physiological states, including pregnancy status. We hypothesized that circulating exosome-derived miRNAs might be used to differentiate the pregnancy status as early as several days after insemination in pigs. To test this hypothesis, we randomly assigned pigs for artificial insemination with fertile or dead semen (control group). Serum samples were obtained from pregnant pigs on days 9, 12, and 15 after insemination and from non-pregnant pigs on days 0, 9, 12, and 15 after insemination. Exosomes were isolated for RNA extraction. The exosomal RNA samples from pigs on day 9 of the estrus cycle and pregnancy were used for small-RNA sequencing. A total 321 miRNAs were identified in all samples. Twenty eight differentially abundant miRNAs were identified between the pregnant and control groups. miRNAs with | log2 (fold change)| > 2 from sequencing results were selected for validation by quantitative reverse-transcription-polymerase chain reaction (RT-qPCR) in larger samples. Finally two upregulated miRNAs (miR-92b-3p and miR-17-5p) in the pregnant groups (on days 9, 12, and 15 of pregnancy) were confirmed by RT-qPCR. In summary, we have successfully identified circulating exosomal miRNA profiles in the serum of pigs in early pregnancy. miR-92b-3p and miR-17-5p could be used as potential circulating biomarkers for early pregnancy diagnosis. Frontiers Media S.A. 2020-05-26 /pmc/articles/PMC7264423/ /pubmed/32528535 http://dx.doi.org/10.3389/fgene.2020.00536 Text en Copyright © 2020 Zhou, Cai, Meng, Xu, He, Hu, Zheng, Huang, Xu, Gu, Hu, Wu and Hong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Zhou, Chen Cai, Gengyuan Meng, Fanming Xu, Zhiqian He, Yanjuan Hu, Qun Zheng, Enqin Huang, Sixiu Xu, Zheng Gu, Ting Hu, Bin Wu, Zhenfang Hong, Linjun Deep-Sequencing Identification of MicroRNA Biomarkers in Serum Exosomes for Early Pig Pregnancy |
title | Deep-Sequencing Identification of MicroRNA Biomarkers in Serum Exosomes for Early Pig Pregnancy |
title_full | Deep-Sequencing Identification of MicroRNA Biomarkers in Serum Exosomes for Early Pig Pregnancy |
title_fullStr | Deep-Sequencing Identification of MicroRNA Biomarkers in Serum Exosomes for Early Pig Pregnancy |
title_full_unstemmed | Deep-Sequencing Identification of MicroRNA Biomarkers in Serum Exosomes for Early Pig Pregnancy |
title_short | Deep-Sequencing Identification of MicroRNA Biomarkers in Serum Exosomes for Early Pig Pregnancy |
title_sort | deep-sequencing identification of microrna biomarkers in serum exosomes for early pig pregnancy |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264423/ https://www.ncbi.nlm.nih.gov/pubmed/32528535 http://dx.doi.org/10.3389/fgene.2020.00536 |
work_keys_str_mv | AT zhouchen deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT caigengyuan deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT mengfanming deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT xuzhiqian deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT heyanjuan deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT huqun deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT zhengenqin deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT huangsixiu deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT xuzheng deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT guting deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT hubin deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT wuzhenfang deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy AT honglinjun deepsequencingidentificationofmicrornabiomarkersinserumexosomesforearlypigpregnancy |