Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows

BACKGROUND: Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. Multiple approaches for optimal sample dissociation and storage of single cells have been proposed as have single-nucl...

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Detalles Bibliográficos
Autores principales: Denisenko, Elena, Guo, Belinda B., Jones, Matthew, Hou, Rui, de Kock, Leanne, Lassmann, Timo, Poppe, Daniel, Clément, Olivier, Simmons, Rebecca K., Lister, Ryan, Forrest, Alistair R. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265231/
https://www.ncbi.nlm.nih.gov/pubmed/32487174
http://dx.doi.org/10.1186/s13059-020-02048-6

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