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Independent anterograde transport and retrograde cotransport of domain components of myelinated axons

Neurons are highly polarized cells organized into functionally and molecularly distinct domains. A key question is whether the multiprotein complexes that comprise these domains are preassembled, transported, and inserted as a complex or whether their components are transported independently and ass...

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Autores principales: Bekku, Yoko, Salzer, James L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265310/
https://www.ncbi.nlm.nih.gov/pubmed/32289157
http://dx.doi.org/10.1083/jcb.201906071
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author Bekku, Yoko
Salzer, James L.
author_facet Bekku, Yoko
Salzer, James L.
author_sort Bekku, Yoko
collection PubMed
description Neurons are highly polarized cells organized into functionally and molecularly distinct domains. A key question is whether the multiprotein complexes that comprise these domains are preassembled, transported, and inserted as a complex or whether their components are transported independently and assemble locally. Here, we have dynamically imaged, in pairwise combinations, the vesicular transport of fluorescently tagged components of the nodes of Ranvier and other myelinated axonal domains in sensory neurons cultured alone or together with Schwann cells at the onset of myelination. In general, most proteins are transported independently in the anterograde direction. In contrast, there is substantial cotransport of proteins from distinct domains in the retrograde direction likely due to coendocytosis along the axon. Early myelination did not substantially change these patterns of transport, although it increased the overall numbers of axonal transport vesicles. Our results indicate domain components are transported in separate vesicles for local assembly, not as preformed complexes, and implicate endocytosis along axons as a mechanism of clearance.
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spelling pubmed-72653102020-12-01 Independent anterograde transport and retrograde cotransport of domain components of myelinated axons Bekku, Yoko Salzer, James L. J Cell Biol Report Neurons are highly polarized cells organized into functionally and molecularly distinct domains. A key question is whether the multiprotein complexes that comprise these domains are preassembled, transported, and inserted as a complex or whether their components are transported independently and assemble locally. Here, we have dynamically imaged, in pairwise combinations, the vesicular transport of fluorescently tagged components of the nodes of Ranvier and other myelinated axonal domains in sensory neurons cultured alone or together with Schwann cells at the onset of myelination. In general, most proteins are transported independently in the anterograde direction. In contrast, there is substantial cotransport of proteins from distinct domains in the retrograde direction likely due to coendocytosis along the axon. Early myelination did not substantially change these patterns of transport, although it increased the overall numbers of axonal transport vesicles. Our results indicate domain components are transported in separate vesicles for local assembly, not as preformed complexes, and implicate endocytosis along axons as a mechanism of clearance. Rockefeller University Press 2020-04-14 /pmc/articles/PMC7265310/ /pubmed/32289157 http://dx.doi.org/10.1083/jcb.201906071 Text en © 2020 Bekku and Salzer http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Report
Bekku, Yoko
Salzer, James L.
Independent anterograde transport and retrograde cotransport of domain components of myelinated axons
title Independent anterograde transport and retrograde cotransport of domain components of myelinated axons
title_full Independent anterograde transport and retrograde cotransport of domain components of myelinated axons
title_fullStr Independent anterograde transport and retrograde cotransport of domain components of myelinated axons
title_full_unstemmed Independent anterograde transport and retrograde cotransport of domain components of myelinated axons
title_short Independent anterograde transport and retrograde cotransport of domain components of myelinated axons
title_sort independent anterograde transport and retrograde cotransport of domain components of myelinated axons
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265310/
https://www.ncbi.nlm.nih.gov/pubmed/32289157
http://dx.doi.org/10.1083/jcb.201906071
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