CRISPR-Cas12a–assisted PCR tagging of mammalian genes

Here we describe a time-efficient strategy for endogenous C-terminal gene tagging in mammalian tissue culture cells. An online platform is used to design two long gene-specific oligonucleotides for PCR with generic template cassettes to create linear dsDNA donors, termed PCR cassettes. PCR cassettes...

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Detalles Bibliográficos
Autores principales: Fueller, Julia, Herbst, Konrad, Meurer, Matthias, Gubicza, Krisztina, Kurtulmus, Bahtiyar, Knopf, Julia D., Kirrmaier, Daniel, Buchmuller, Benjamin C., Pereira, Gislene, Lemberg, Marius K., Knop, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2020
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265327/
https://www.ncbi.nlm.nih.gov/pubmed/32406907
http://dx.doi.org/10.1083/jcb.201910210

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