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Drug response analysis for scaffold-free cardiac constructs fabricated using bio-3D printer
Cardiac constructs fabricated using human induced pluripotent stem cells-derived cardiomyocytes (iPSCs-CMs) are useful for evaluating the cardiotoxicity of and cardiac response to new drugs. Previously, we fabricated scaffold-free three-dimensional (3D) tubular cardiac constructs using a bio-3D prin...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265390/ https://www.ncbi.nlm.nih.gov/pubmed/32487993 http://dx.doi.org/10.1038/s41598-020-65681-y |
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author | Arai, Kenichi Murata, Daiki Takao, Shoko Nakamura, Anna Itoh, Manabu Kitsuka, Takahiro Nakayama, Koichi |
author_facet | Arai, Kenichi Murata, Daiki Takao, Shoko Nakamura, Anna Itoh, Manabu Kitsuka, Takahiro Nakayama, Koichi |
author_sort | Arai, Kenichi |
collection | PubMed |
description | Cardiac constructs fabricated using human induced pluripotent stem cells-derived cardiomyocytes (iPSCs-CMs) are useful for evaluating the cardiotoxicity of and cardiac response to new drugs. Previously, we fabricated scaffold-free three-dimensional (3D) tubular cardiac constructs using a bio-3D printer, which can load cardiac spheroids onto a needle array. In this study, we developed a method to measure the contractile force and to evaluate the drug response in cardiac constructs. Specifically, we measured the movement of the needle tip upon contraction of the cardiac constructs on the needle array. The contractile force and beating rate of the cardiac constructs were evaluated by analysing changes in the movement of the needle tip. To evaluate the drug response, contractile properties were measured following treatment with isoproterenol, propranolol, or blebbistatin, in which the movement of the needle tip was increased following isoproterenol treatment, but was decreased following propranolol or blebbistain, treatments. To evaluate cardiotoxicity, contraction and cell viability of the cardiac constructs were measured following doxorubicin treatment. Cell viability was found to decrease with decreasing movement of the needle tip following doxorubicin treatment. Collectively, our results show that this method can aid in evaluating the contractile force of cardiac constructs. |
format | Online Article Text |
id | pubmed-7265390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72653902020-06-05 Drug response analysis for scaffold-free cardiac constructs fabricated using bio-3D printer Arai, Kenichi Murata, Daiki Takao, Shoko Nakamura, Anna Itoh, Manabu Kitsuka, Takahiro Nakayama, Koichi Sci Rep Article Cardiac constructs fabricated using human induced pluripotent stem cells-derived cardiomyocytes (iPSCs-CMs) are useful for evaluating the cardiotoxicity of and cardiac response to new drugs. Previously, we fabricated scaffold-free three-dimensional (3D) tubular cardiac constructs using a bio-3D printer, which can load cardiac spheroids onto a needle array. In this study, we developed a method to measure the contractile force and to evaluate the drug response in cardiac constructs. Specifically, we measured the movement of the needle tip upon contraction of the cardiac constructs on the needle array. The contractile force and beating rate of the cardiac constructs were evaluated by analysing changes in the movement of the needle tip. To evaluate the drug response, contractile properties were measured following treatment with isoproterenol, propranolol, or blebbistatin, in which the movement of the needle tip was increased following isoproterenol treatment, but was decreased following propranolol or blebbistain, treatments. To evaluate cardiotoxicity, contraction and cell viability of the cardiac constructs were measured following doxorubicin treatment. Cell viability was found to decrease with decreasing movement of the needle tip following doxorubicin treatment. Collectively, our results show that this method can aid in evaluating the contractile force of cardiac constructs. Nature Publishing Group UK 2020-06-02 /pmc/articles/PMC7265390/ /pubmed/32487993 http://dx.doi.org/10.1038/s41598-020-65681-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Arai, Kenichi Murata, Daiki Takao, Shoko Nakamura, Anna Itoh, Manabu Kitsuka, Takahiro Nakayama, Koichi Drug response analysis for scaffold-free cardiac constructs fabricated using bio-3D printer |
title | Drug response analysis for scaffold-free cardiac constructs fabricated using bio-3D printer |
title_full | Drug response analysis for scaffold-free cardiac constructs fabricated using bio-3D printer |
title_fullStr | Drug response analysis for scaffold-free cardiac constructs fabricated using bio-3D printer |
title_full_unstemmed | Drug response analysis for scaffold-free cardiac constructs fabricated using bio-3D printer |
title_short | Drug response analysis for scaffold-free cardiac constructs fabricated using bio-3D printer |
title_sort | drug response analysis for scaffold-free cardiac constructs fabricated using bio-3d printer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265390/ https://www.ncbi.nlm.nih.gov/pubmed/32487993 http://dx.doi.org/10.1038/s41598-020-65681-y |
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