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AFM Imaging of Protein Aggregation in Studying the Impact of Knotted Electromagnetic Field on A Peroxidase
The phenomenon of knotted electromagnetic field (KEMF) is now actively studied, as such fields are characterized by a nontrivial topology. The research in this field is mainly aimed at technical applications – for instance, the development of efficient communication systems. Until present, however,...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265551/ https://www.ncbi.nlm.nih.gov/pubmed/32488177 http://dx.doi.org/10.1038/s41598-020-65888-z |
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author | Ivanov, Yuri D. Pleshakova, Tatyana O. Shumov, Ivan D. Kozlov, Andrey F. Ivanova, Irina A. Valueva, Anastasia A. Tatur, Vadim Yu. Smelov, Mikhail V. Ivanova, Nina D. Ziborov, Vadim S. |
author_facet | Ivanov, Yuri D. Pleshakova, Tatyana O. Shumov, Ivan D. Kozlov, Andrey F. Ivanova, Irina A. Valueva, Anastasia A. Tatur, Vadim Yu. Smelov, Mikhail V. Ivanova, Nina D. Ziborov, Vadim S. |
author_sort | Ivanov, Yuri D. |
collection | PubMed |
description | The phenomenon of knotted electromagnetic field (KEMF) is now actively studied, as such fields are characterized by a nontrivial topology. The research in this field is mainly aimed at technical applications – for instance, the development of efficient communication systems. Until present, however, the influence of KEMF on biological objects (including enzyme systems) was not considered. Herein, we have studied the influence of KEMF on the aggregation and enzymatic activity of a protein with the example of horseradish peroxidase (HRP). The test HRP solution was irradiated in KEMF (the radiation power density was 10(−12) W/cm(2) at 2.3 GHz frequency) for 40 min. After the irradiation, the aggregation of HRP was examined by atomic force microscopy (AFM) at the single-molecule level. The enzymatic activity was monitored by conventional spectrophotometry. It has been demonstrated that an increased aggregation of HRP, adsorbed on the AFM substrate surface, was observed after irradiation of the protein sample in KEMF with low (10(−12) W/cm(2)) radiation power density; at the same time, the enzymatic activity remained unchanged. The results obtained herein can be used in the development of models describing the interaction of enzymes with electromagnetic field. The obtained data can also be of importance considering possible pathological factors that can take place upon the influence of KEMF on biological objects— for instance, changes in hemodynamics due to increased protein aggregation are possible; the functionality of protein complexes can also be affected by aggregation of their protein subunits. These effects should also be taken into account in the development of novel highly sensitive systems for human serological diagnostics of breast cancer, prostate cancer, brain cancer and other oncological pathologies, and for diagnostics of diseases in animals, and crops. |
format | Online Article Text |
id | pubmed-7265551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72655512020-06-05 AFM Imaging of Protein Aggregation in Studying the Impact of Knotted Electromagnetic Field on A Peroxidase Ivanov, Yuri D. Pleshakova, Tatyana O. Shumov, Ivan D. Kozlov, Andrey F. Ivanova, Irina A. Valueva, Anastasia A. Tatur, Vadim Yu. Smelov, Mikhail V. Ivanova, Nina D. Ziborov, Vadim S. Sci Rep Article The phenomenon of knotted electromagnetic field (KEMF) is now actively studied, as such fields are characterized by a nontrivial topology. The research in this field is mainly aimed at technical applications – for instance, the development of efficient communication systems. Until present, however, the influence of KEMF on biological objects (including enzyme systems) was not considered. Herein, we have studied the influence of KEMF on the aggregation and enzymatic activity of a protein with the example of horseradish peroxidase (HRP). The test HRP solution was irradiated in KEMF (the radiation power density was 10(−12) W/cm(2) at 2.3 GHz frequency) for 40 min. After the irradiation, the aggregation of HRP was examined by atomic force microscopy (AFM) at the single-molecule level. The enzymatic activity was monitored by conventional spectrophotometry. It has been demonstrated that an increased aggregation of HRP, adsorbed on the AFM substrate surface, was observed after irradiation of the protein sample in KEMF with low (10(−12) W/cm(2)) radiation power density; at the same time, the enzymatic activity remained unchanged. The results obtained herein can be used in the development of models describing the interaction of enzymes with electromagnetic field. The obtained data can also be of importance considering possible pathological factors that can take place upon the influence of KEMF on biological objects— for instance, changes in hemodynamics due to increased protein aggregation are possible; the functionality of protein complexes can also be affected by aggregation of their protein subunits. These effects should also be taken into account in the development of novel highly sensitive systems for human serological diagnostics of breast cancer, prostate cancer, brain cancer and other oncological pathologies, and for diagnostics of diseases in animals, and crops. Nature Publishing Group UK 2020-06-02 /pmc/articles/PMC7265551/ /pubmed/32488177 http://dx.doi.org/10.1038/s41598-020-65888-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ivanov, Yuri D. Pleshakova, Tatyana O. Shumov, Ivan D. Kozlov, Andrey F. Ivanova, Irina A. Valueva, Anastasia A. Tatur, Vadim Yu. Smelov, Mikhail V. Ivanova, Nina D. Ziborov, Vadim S. AFM Imaging of Protein Aggregation in Studying the Impact of Knotted Electromagnetic Field on A Peroxidase |
title | AFM Imaging of Protein Aggregation in Studying the Impact of Knotted Electromagnetic Field on A Peroxidase |
title_full | AFM Imaging of Protein Aggregation in Studying the Impact of Knotted Electromagnetic Field on A Peroxidase |
title_fullStr | AFM Imaging of Protein Aggregation in Studying the Impact of Knotted Electromagnetic Field on A Peroxidase |
title_full_unstemmed | AFM Imaging of Protein Aggregation in Studying the Impact of Knotted Electromagnetic Field on A Peroxidase |
title_short | AFM Imaging of Protein Aggregation in Studying the Impact of Knotted Electromagnetic Field on A Peroxidase |
title_sort | afm imaging of protein aggregation in studying the impact of knotted electromagnetic field on a peroxidase |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265551/ https://www.ncbi.nlm.nih.gov/pubmed/32488177 http://dx.doi.org/10.1038/s41598-020-65888-z |
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