Cargando…

Comparative transcriptomics of an arctic foundation species, tussock cottongrass (Eriophorum vaginatum), during an extreme heat event

Tussock cottongrass (Eriophorum vaginatum) is a foundation species for much of the arctic moist acidic tundra, which is currently experiencing extreme effects of climate change. The Arctic is facing higher summer temperatures and extreme weather events are becoming more common. We used Illumina RNA-...

Descripción completa

Detalles Bibliográficos
Autores principales: Mohl, Jonathon E., Fetcher, Ned, Stunz, Elizabeth, Tang, Jianwu, Moody, Michael L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265556/
https://www.ncbi.nlm.nih.gov/pubmed/32488082
http://dx.doi.org/10.1038/s41598-020-65693-8
_version_ 1783541158655295488
author Mohl, Jonathon E.
Fetcher, Ned
Stunz, Elizabeth
Tang, Jianwu
Moody, Michael L.
author_facet Mohl, Jonathon E.
Fetcher, Ned
Stunz, Elizabeth
Tang, Jianwu
Moody, Michael L.
author_sort Mohl, Jonathon E.
collection PubMed
description Tussock cottongrass (Eriophorum vaginatum) is a foundation species for much of the arctic moist acidic tundra, which is currently experiencing extreme effects of climate change. The Arctic is facing higher summer temperatures and extreme weather events are becoming more common. We used Illumina RNA-Seq to analyse cDNA libraries for differential expression of genes from leaves of ecologically well-characterized ecotypes of tussock cottongrass found along a latitudinal gradient in the Alaskan Arctic and transplanted into a common garden. Plant sampling was performed on a typical summer day and during an extreme heat event. We obtained a de novo assembly that contained 423,353 unigenes. There were 363 unigenes up-regulated and 1,117 down-regulated among all ecotypes examined during the extreme heat event. Of these, 26 HSP unigenes had >log2-fold up-regulation. Several TFs associated with heat stress in previous studies were identified that had >log2-fold up- or down-regulation during the extreme heat event (e.g., DREB, NAC). There was consistent variation in DEGs among ecotypes, but not specifically related to whether plants originated from taiga or tundra ecosystems. As the climate changes it is essential to determine ecotypic diversity at the genomic level, especially for widespread species that impact ecosystem function.
format Online
Article
Text
id pubmed-7265556
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-72655562020-06-05 Comparative transcriptomics of an arctic foundation species, tussock cottongrass (Eriophorum vaginatum), during an extreme heat event Mohl, Jonathon E. Fetcher, Ned Stunz, Elizabeth Tang, Jianwu Moody, Michael L. Sci Rep Article Tussock cottongrass (Eriophorum vaginatum) is a foundation species for much of the arctic moist acidic tundra, which is currently experiencing extreme effects of climate change. The Arctic is facing higher summer temperatures and extreme weather events are becoming more common. We used Illumina RNA-Seq to analyse cDNA libraries for differential expression of genes from leaves of ecologically well-characterized ecotypes of tussock cottongrass found along a latitudinal gradient in the Alaskan Arctic and transplanted into a common garden. Plant sampling was performed on a typical summer day and during an extreme heat event. We obtained a de novo assembly that contained 423,353 unigenes. There were 363 unigenes up-regulated and 1,117 down-regulated among all ecotypes examined during the extreme heat event. Of these, 26 HSP unigenes had >log2-fold up-regulation. Several TFs associated with heat stress in previous studies were identified that had >log2-fold up- or down-regulation during the extreme heat event (e.g., DREB, NAC). There was consistent variation in DEGs among ecotypes, but not specifically related to whether plants originated from taiga or tundra ecosystems. As the climate changes it is essential to determine ecotypic diversity at the genomic level, especially for widespread species that impact ecosystem function. Nature Publishing Group UK 2020-06-02 /pmc/articles/PMC7265556/ /pubmed/32488082 http://dx.doi.org/10.1038/s41598-020-65693-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mohl, Jonathon E.
Fetcher, Ned
Stunz, Elizabeth
Tang, Jianwu
Moody, Michael L.
Comparative transcriptomics of an arctic foundation species, tussock cottongrass (Eriophorum vaginatum), during an extreme heat event
title Comparative transcriptomics of an arctic foundation species, tussock cottongrass (Eriophorum vaginatum), during an extreme heat event
title_full Comparative transcriptomics of an arctic foundation species, tussock cottongrass (Eriophorum vaginatum), during an extreme heat event
title_fullStr Comparative transcriptomics of an arctic foundation species, tussock cottongrass (Eriophorum vaginatum), during an extreme heat event
title_full_unstemmed Comparative transcriptomics of an arctic foundation species, tussock cottongrass (Eriophorum vaginatum), during an extreme heat event
title_short Comparative transcriptomics of an arctic foundation species, tussock cottongrass (Eriophorum vaginatum), during an extreme heat event
title_sort comparative transcriptomics of an arctic foundation species, tussock cottongrass (eriophorum vaginatum), during an extreme heat event
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265556/
https://www.ncbi.nlm.nih.gov/pubmed/32488082
http://dx.doi.org/10.1038/s41598-020-65693-8
work_keys_str_mv AT mohljonathone comparativetranscriptomicsofanarcticfoundationspeciestussockcottongrasseriophorumvaginatumduringanextremeheatevent
AT fetcherned comparativetranscriptomicsofanarcticfoundationspeciestussockcottongrasseriophorumvaginatumduringanextremeheatevent
AT stunzelizabeth comparativetranscriptomicsofanarcticfoundationspeciestussockcottongrasseriophorumvaginatumduringanextremeheatevent
AT tangjianwu comparativetranscriptomicsofanarcticfoundationspeciestussockcottongrasseriophorumvaginatumduringanextremeheatevent
AT moodymichaell comparativetranscriptomicsofanarcticfoundationspeciestussockcottongrasseriophorumvaginatumduringanextremeheatevent