Cargando…

Effect of tea root-derived proanthocyanidin fractions on protection of dentin collagen

OBJECTIVES: Proanthocyanidins (PAs) have been widely used as effective agents for dentin collagen cross-linking to enhance the biomechanics and biostability of dentin in vitro. However, the effects and protective mechanisms of various tea root-derived PA components on dentin remain undefined. This s...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Honglin, Xie, Bingqing, Wang, Yue, Cui, Yayun, Yang, Hui, Wang, Xiaoting, Yang, Xi, Bao, Guan-Hu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265569/
https://www.ncbi.nlm.nih.gov/pubmed/31818172
http://dx.doi.org/10.1177/0300060519891303
_version_ 1783541161468624896
author Yang, Honglin
Xie, Bingqing
Wang, Yue
Cui, Yayun
Yang, Hui
Wang, Xiaoting
Yang, Xi
Bao, Guan-Hu
author_facet Yang, Honglin
Xie, Bingqing
Wang, Yue
Cui, Yayun
Yang, Hui
Wang, Xiaoting
Yang, Xi
Bao, Guan-Hu
author_sort Yang, Honglin
collection PubMed
description OBJECTIVES: Proanthocyanidins (PAs) have been widely used as effective agents for dentin collagen cross-linking to enhance the biomechanics and biostability of dentin in vitro. However, the effects and protective mechanisms of various tea root-derived PA components on dentin remain undefined. This study evaluated the effects of these tea root-derived PA components on dentin biomechanics and biostability. METHODS: In this study, ethyl acetate and n-butyl alcohol were used to extract PAs with different degrees of polymerization from tea roots; the effects of these PA extracts on dentin were evaluated. RESULTS: Dentin was treated with glutaraldehyde, ethyl acetate, n-butyl alcohol, or water. PAs with a high degree of polymerization, extracted using n-butyl alcohol, were able to more effectively improve dentin collagen cross-linking, increase resistance to bacterial collagenase digestion, and enhance dentin elasticity, relative to treatment with glutaraldehyde or PAs with a low degree of polymerization (extracted using ethyl acetate). Additionally, treatment with aqueous extract of tea roots was detrimental to dentin stability and function. CONCLUSIONS: PAs with a high degree of polymerization were effective for dentin protection and restoration in vitro, suggesting clinical treatment potential for tea root-derived PAs.
format Online
Article
Text
id pubmed-7265569
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-72655692020-06-10 Effect of tea root-derived proanthocyanidin fractions on protection of dentin collagen Yang, Honglin Xie, Bingqing Wang, Yue Cui, Yayun Yang, Hui Wang, Xiaoting Yang, Xi Bao, Guan-Hu J Int Med Res Pre-Clinical Research Report OBJECTIVES: Proanthocyanidins (PAs) have been widely used as effective agents for dentin collagen cross-linking to enhance the biomechanics and biostability of dentin in vitro. However, the effects and protective mechanisms of various tea root-derived PA components on dentin remain undefined. This study evaluated the effects of these tea root-derived PA components on dentin biomechanics and biostability. METHODS: In this study, ethyl acetate and n-butyl alcohol were used to extract PAs with different degrees of polymerization from tea roots; the effects of these PA extracts on dentin were evaluated. RESULTS: Dentin was treated with glutaraldehyde, ethyl acetate, n-butyl alcohol, or water. PAs with a high degree of polymerization, extracted using n-butyl alcohol, were able to more effectively improve dentin collagen cross-linking, increase resistance to bacterial collagenase digestion, and enhance dentin elasticity, relative to treatment with glutaraldehyde or PAs with a low degree of polymerization (extracted using ethyl acetate). Additionally, treatment with aqueous extract of tea roots was detrimental to dentin stability and function. CONCLUSIONS: PAs with a high degree of polymerization were effective for dentin protection and restoration in vitro, suggesting clinical treatment potential for tea root-derived PAs. SAGE Publications 2019-12-09 /pmc/articles/PMC7265569/ /pubmed/31818172 http://dx.doi.org/10.1177/0300060519891303 Text en © The Author(s) 2019 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Pre-Clinical Research Report
Yang, Honglin
Xie, Bingqing
Wang, Yue
Cui, Yayun
Yang, Hui
Wang, Xiaoting
Yang, Xi
Bao, Guan-Hu
Effect of tea root-derived proanthocyanidin fractions on protection of dentin collagen
title Effect of tea root-derived proanthocyanidin fractions on protection of dentin collagen
title_full Effect of tea root-derived proanthocyanidin fractions on protection of dentin collagen
title_fullStr Effect of tea root-derived proanthocyanidin fractions on protection of dentin collagen
title_full_unstemmed Effect of tea root-derived proanthocyanidin fractions on protection of dentin collagen
title_short Effect of tea root-derived proanthocyanidin fractions on protection of dentin collagen
title_sort effect of tea root-derived proanthocyanidin fractions on protection of dentin collagen
topic Pre-Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265569/
https://www.ncbi.nlm.nih.gov/pubmed/31818172
http://dx.doi.org/10.1177/0300060519891303
work_keys_str_mv AT yanghonglin effectoftearootderivedproanthocyanidinfractionsonprotectionofdentincollagen
AT xiebingqing effectoftearootderivedproanthocyanidinfractionsonprotectionofdentincollagen
AT wangyue effectoftearootderivedproanthocyanidinfractionsonprotectionofdentincollagen
AT cuiyayun effectoftearootderivedproanthocyanidinfractionsonprotectionofdentincollagen
AT yanghui effectoftearootderivedproanthocyanidinfractionsonprotectionofdentincollagen
AT wangxiaoting effectoftearootderivedproanthocyanidinfractionsonprotectionofdentincollagen
AT yangxi effectoftearootderivedproanthocyanidinfractionsonprotectionofdentincollagen
AT baoguanhu effectoftearootderivedproanthocyanidinfractionsonprotectionofdentincollagen