Superinfection exclusion studies using West Nile virus and Culex flavivirus strains from Argentina

In Argentina, many Flavivirus were recognised including West Nile virus (WNV). During 2009 several strains of Culex Flavivirus (CxFV), an insect-specific flavivirus, were isolated in the same region where circulation of WNV was detected. Hence, the objective of this study was to analyse the effect o...

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Autores principales: Goenaga, Silvina, Goenaga, Julieta, Boaglio, Estefanía Raquel, Enria, Delia Alcira, Levis, Silvana del Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265678/
https://www.ncbi.nlm.nih.gov/pubmed/32520074
http://dx.doi.org/10.1590/0074-02760200012
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author Goenaga, Silvina
Goenaga, Julieta
Boaglio, Estefanía Raquel
Enria, Delia Alcira
Levis, Silvana del Carmen
author_facet Goenaga, Silvina
Goenaga, Julieta
Boaglio, Estefanía Raquel
Enria, Delia Alcira
Levis, Silvana del Carmen
author_sort Goenaga, Silvina
collection PubMed
description In Argentina, many Flavivirus were recognised including West Nile virus (WNV). During 2009 several strains of Culex Flavivirus (CxFV), an insect-specific flavivirus, were isolated in the same region where circulation of WNV was detected. Hence, the objective of this study was to analyse the effect of co-infection in vitro assays using CxFV and WNV Argentinean strains in order to evaluate if CxFV could affect WNV replication. Our results showed that WNV replication was suppressed when multiplicity of infection (MOI) for CxFV was 10 or 100 times higher than WNV. Nevertheless, in vivo assays are necessary in order to evaluate the superinfection exclusion potential.
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spelling pubmed-72656782020-06-10 Superinfection exclusion studies using West Nile virus and Culex flavivirus strains from Argentina Goenaga, Silvina Goenaga, Julieta Boaglio, Estefanía Raquel Enria, Delia Alcira Levis, Silvana del Carmen Mem Inst Oswaldo Cruz Short Communication In Argentina, many Flavivirus were recognised including West Nile virus (WNV). During 2009 several strains of Culex Flavivirus (CxFV), an insect-specific flavivirus, were isolated in the same region where circulation of WNV was detected. Hence, the objective of this study was to analyse the effect of co-infection in vitro assays using CxFV and WNV Argentinean strains in order to evaluate if CxFV could affect WNV replication. Our results showed that WNV replication was suppressed when multiplicity of infection (MOI) for CxFV was 10 or 100 times higher than WNV. Nevertheless, in vivo assays are necessary in order to evaluate the superinfection exclusion potential. Instituto Oswaldo Cruz, Ministério da Saúde 2020-06-03 /pmc/articles/PMC7265678/ /pubmed/32520074 http://dx.doi.org/10.1590/0074-02760200012 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
Goenaga, Silvina
Goenaga, Julieta
Boaglio, Estefanía Raquel
Enria, Delia Alcira
Levis, Silvana del Carmen
Superinfection exclusion studies using West Nile virus and Culex flavivirus strains from Argentina
title Superinfection exclusion studies using West Nile virus and Culex flavivirus strains from Argentina
title_full Superinfection exclusion studies using West Nile virus and Culex flavivirus strains from Argentina
title_fullStr Superinfection exclusion studies using West Nile virus and Culex flavivirus strains from Argentina
title_full_unstemmed Superinfection exclusion studies using West Nile virus and Culex flavivirus strains from Argentina
title_short Superinfection exclusion studies using West Nile virus and Culex flavivirus strains from Argentina
title_sort superinfection exclusion studies using west nile virus and culex flavivirus strains from argentina
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265678/
https://www.ncbi.nlm.nih.gov/pubmed/32520074
http://dx.doi.org/10.1590/0074-02760200012
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