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Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations
Rye stem rust caused by Puccinia graminis f. sp. secalis can be found in all European rye growing regions. When the summers are warm and dry, the disease can cause severe yield losses over large areas. To date only little research was done in Europe to trigger resistance breeding. To our knowledge,...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265987/ https://www.ncbi.nlm.nih.gov/pubmed/32528509 http://dx.doi.org/10.3389/fpls.2020.00667 |
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author | Gruner, Paul Schmitt, Anne-Kristin Flath, Kerstin Schmiedchen, Brigitta Eifler, Jakob Gordillo, Andres Schmidt, Malthe Korzun, Viktor Fromme, Franz-Joachim Siekmann, Dörthe Tratwal, Anna Danielewicz, Jakub Korbas, Marek Marciniak, Karol Krysztofik, Renata Niewińska, Małgorzata Koch, Silvia Piepho, Hans-Peter Miedaner, Thomas |
author_facet | Gruner, Paul Schmitt, Anne-Kristin Flath, Kerstin Schmiedchen, Brigitta Eifler, Jakob Gordillo, Andres Schmidt, Malthe Korzun, Viktor Fromme, Franz-Joachim Siekmann, Dörthe Tratwal, Anna Danielewicz, Jakub Korbas, Marek Marciniak, Karol Krysztofik, Renata Niewińska, Małgorzata Koch, Silvia Piepho, Hans-Peter Miedaner, Thomas |
author_sort | Gruner, Paul |
collection | PubMed |
description | Rye stem rust caused by Puccinia graminis f. sp. secalis can be found in all European rye growing regions. When the summers are warm and dry, the disease can cause severe yield losses over large areas. To date only little research was done in Europe to trigger resistance breeding. To our knowledge, all varieties currently registered in Germany are susceptible. In this study, three biparental populations of inbred lines and one testcross population developed for mapping resistance were investigated. Over 2 years, 68–70 genotypes per population were tested, each in three locations. Combining the phenotypic data with genotyping results of a custom 10k Infinium iSelect single nucleotide polymorphism (SNP) array, we identified both quantitatively inherited adult plant resistance and monogenic all-stage resistance. A single resistance gene, tentatively named Pgs1, located at the distal end of chromosome 7R, could be identified in two independently developed populations. With high probability, it is closely linked to a nucleotide-binding leucine-rich repeat (NB-LRR) resistance gene homolog. A marker for a competitive allele-specific polymerase chain reaction (KASP) genotyping assay was designed that could explain 73 and 97% of the genetic variance in each of both populations, respectively. Additional investigation of naturally occurring rye leaf rust (caused by Puccinia recondita ROEBERGE) revealed a gene complex on chromosome 7R. The gene Pgs1 and further identified quantitative trait loci (QTL) have high potential to be used for breeding stem rust resistant rye. |
format | Online Article Text |
id | pubmed-7265987 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72659872020-06-10 Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations Gruner, Paul Schmitt, Anne-Kristin Flath, Kerstin Schmiedchen, Brigitta Eifler, Jakob Gordillo, Andres Schmidt, Malthe Korzun, Viktor Fromme, Franz-Joachim Siekmann, Dörthe Tratwal, Anna Danielewicz, Jakub Korbas, Marek Marciniak, Karol Krysztofik, Renata Niewińska, Małgorzata Koch, Silvia Piepho, Hans-Peter Miedaner, Thomas Front Plant Sci Plant Science Rye stem rust caused by Puccinia graminis f. sp. secalis can be found in all European rye growing regions. When the summers are warm and dry, the disease can cause severe yield losses over large areas. To date only little research was done in Europe to trigger resistance breeding. To our knowledge, all varieties currently registered in Germany are susceptible. In this study, three biparental populations of inbred lines and one testcross population developed for mapping resistance were investigated. Over 2 years, 68–70 genotypes per population were tested, each in three locations. Combining the phenotypic data with genotyping results of a custom 10k Infinium iSelect single nucleotide polymorphism (SNP) array, we identified both quantitatively inherited adult plant resistance and monogenic all-stage resistance. A single resistance gene, tentatively named Pgs1, located at the distal end of chromosome 7R, could be identified in two independently developed populations. With high probability, it is closely linked to a nucleotide-binding leucine-rich repeat (NB-LRR) resistance gene homolog. A marker for a competitive allele-specific polymerase chain reaction (KASP) genotyping assay was designed that could explain 73 and 97% of the genetic variance in each of both populations, respectively. Additional investigation of naturally occurring rye leaf rust (caused by Puccinia recondita ROEBERGE) revealed a gene complex on chromosome 7R. The gene Pgs1 and further identified quantitative trait loci (QTL) have high potential to be used for breeding stem rust resistant rye. Frontiers Media S.A. 2020-05-26 /pmc/articles/PMC7265987/ /pubmed/32528509 http://dx.doi.org/10.3389/fpls.2020.00667 Text en Copyright © 2020 Gruner, Schmitt, Flath, Schmiedchen, Eifler, Gordillo, Schmidt, Korzun, Fromme, Siekmann, Tratwal, Danielewicz, Korbas, Marciniak, Krysztofik, Niewińska, Koch, Piepho and Miedaner. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Gruner, Paul Schmitt, Anne-Kristin Flath, Kerstin Schmiedchen, Brigitta Eifler, Jakob Gordillo, Andres Schmidt, Malthe Korzun, Viktor Fromme, Franz-Joachim Siekmann, Dörthe Tratwal, Anna Danielewicz, Jakub Korbas, Marek Marciniak, Karol Krysztofik, Renata Niewińska, Małgorzata Koch, Silvia Piepho, Hans-Peter Miedaner, Thomas Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations |
title | Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations |
title_full | Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations |
title_fullStr | Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations |
title_full_unstemmed | Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations |
title_short | Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations |
title_sort | mapping stem rust (puccinia graminis f. sp. secalis) resistance in self-fertile winter rye populations |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265987/ https://www.ncbi.nlm.nih.gov/pubmed/32528509 http://dx.doi.org/10.3389/fpls.2020.00667 |
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