Cargando…
Wheat germ agglutinin–conjugated fluorescent pH sensors for visualizing proton fluxes
Small-molecule fluorescent wheat germ agglutinin (WGA) conjugates are routinely used to demarcate mammalian plasma membranes, because they bind to the cell’s glycocalyx. Here, we describe the derivatization of WGA with a pH-sensitive rhodamine fluorophore (pHRho; pKa = 7) to detect proton channel fl...
Autores principales: | Zhang, Lejie, Zhang, Mei, Bellve, Karl, Fogarty, Kevin E., Castro, Maite A., Brauchi, Sebastian, Kobertz, William R. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266149/ https://www.ncbi.nlm.nih.gov/pubmed/31978216 http://dx.doi.org/10.1085/jgp.201912498 |
Ejemplares similares
-
Calmodulation meta-analysis: Predicting calmodulin binding via canonical motif clustering
por: Mruk, Karen, et al.
Publicado: (2014) -
Immunocytochemical localization of wheat germ agglutinin in wheat
Publicado: (1982) -
A high-throughput fluorescence lifetime-based assay to detect binding of myosin-binding protein C to F-actin
por: Bunch, Thomas A., et al.
Publicado: (2021) -
Influence of Concanavalin A, wheat germ agglutinin, and soybean agglutinin on the fusion of myoblasts in vitro
Publicado: (1975) -
Fractionation of human T lymphocytes on wheat germ agglutinin-sepharose
Publicado: (1976)