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Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients

Royal jelly (RJ), a creamy substance secreted by honeybees, is the exclusive diet for queen bee differentiation and life maintenance. RJ has been used in cosmetics, beverages, medicines, and supplements worldwide. However, allergy is a concerning issue for RJ, especially in atopic dermatitis (AD) an...

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Autores principales: Hata, Taketoshi, Furusawa-Horie, Takako, Arai, Yasuko, Takahashi, Tomoko, Seishima, Mariko, Ichihara, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266330/
https://www.ncbi.nlm.nih.gov/pubmed/32484810
http://dx.doi.org/10.1371/journal.pone.0233707
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author Hata, Taketoshi
Furusawa-Horie, Takako
Arai, Yasuko
Takahashi, Tomoko
Seishima, Mariko
Ichihara, Kenji
author_facet Hata, Taketoshi
Furusawa-Horie, Takako
Arai, Yasuko
Takahashi, Tomoko
Seishima, Mariko
Ichihara, Kenji
author_sort Hata, Taketoshi
collection PubMed
description Royal jelly (RJ), a creamy substance secreted by honeybees, is the exclusive diet for queen bee differentiation and life maintenance. RJ has been used in cosmetics, beverages, medicines, and supplements worldwide. However, allergy is a concerning issue for RJ, especially in atopic dermatitis (AD) and asthma patients. In some cases, allergic reactions are seen after the first intake of RJ, suggesting the existence of allergens cross-reactive with RJ. Information about the cross-reactive allergens is very important for the safe application of RJ; however, study of this cross-reactivity is quite limited. In this study, we attempted to identify allergens cross-reactive with RJ by using serum samples from 30 AD patients who had never been exposed to RJ. In an enzyme-linked immunosorbent assay (ELISA) experiment, RJ-binding IgE antibodies were detected in the serum of 10 out of 30 patients, and their antibody titers ranged from 4- to 2,048-fold dilution ratios. Additionally, 3 AD patients were determined to be positive in a skin-prick test (SPT) with an RJ solution. Significant correlations were observed between the anti-RJ antibody titer and nonspecific IgE and between the anti-RJ antibody titer and the Eczema Area and Severity Index score. We further examined the cross-reactivity between RJ and 14 typical allergens by using an ELISA-inhibition assay and demonstrated that the following 6 allergens showed cross-reactivity with RJ: the European house dust mite (HDM) (Dermatophagoides pteronyssinus), American HDM (Dermatophagoides farinae), snow crab (Chionocetes spp.), edible crab (Cancer pagurus), German cockroach (Blatella germanica), and honeybee venom (Apis mellifera). In conclusion, people with a history of allergic diseases, including AD, asthma, and allergic rhinitis, should be cautioned against consuming RJ products because of the potential for cross-reactive responses to ensure the safe and successful use of RJ supplements.
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spelling pubmed-72663302020-06-10 Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients Hata, Taketoshi Furusawa-Horie, Takako Arai, Yasuko Takahashi, Tomoko Seishima, Mariko Ichihara, Kenji PLoS One Research Article Royal jelly (RJ), a creamy substance secreted by honeybees, is the exclusive diet for queen bee differentiation and life maintenance. RJ has been used in cosmetics, beverages, medicines, and supplements worldwide. However, allergy is a concerning issue for RJ, especially in atopic dermatitis (AD) and asthma patients. In some cases, allergic reactions are seen after the first intake of RJ, suggesting the existence of allergens cross-reactive with RJ. Information about the cross-reactive allergens is very important for the safe application of RJ; however, study of this cross-reactivity is quite limited. In this study, we attempted to identify allergens cross-reactive with RJ by using serum samples from 30 AD patients who had never been exposed to RJ. In an enzyme-linked immunosorbent assay (ELISA) experiment, RJ-binding IgE antibodies were detected in the serum of 10 out of 30 patients, and their antibody titers ranged from 4- to 2,048-fold dilution ratios. Additionally, 3 AD patients were determined to be positive in a skin-prick test (SPT) with an RJ solution. Significant correlations were observed between the anti-RJ antibody titer and nonspecific IgE and between the anti-RJ antibody titer and the Eczema Area and Severity Index score. We further examined the cross-reactivity between RJ and 14 typical allergens by using an ELISA-inhibition assay and demonstrated that the following 6 allergens showed cross-reactivity with RJ: the European house dust mite (HDM) (Dermatophagoides pteronyssinus), American HDM (Dermatophagoides farinae), snow crab (Chionocetes spp.), edible crab (Cancer pagurus), German cockroach (Blatella germanica), and honeybee venom (Apis mellifera). In conclusion, people with a history of allergic diseases, including AD, asthma, and allergic rhinitis, should be cautioned against consuming RJ products because of the potential for cross-reactive responses to ensure the safe and successful use of RJ supplements. Public Library of Science 2020-06-02 /pmc/articles/PMC7266330/ /pubmed/32484810 http://dx.doi.org/10.1371/journal.pone.0233707 Text en © 2020 Hata et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hata, Taketoshi
Furusawa-Horie, Takako
Arai, Yasuko
Takahashi, Tomoko
Seishima, Mariko
Ichihara, Kenji
Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients
title Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients
title_full Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients
title_fullStr Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients
title_full_unstemmed Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients
title_short Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients
title_sort studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266330/
https://www.ncbi.nlm.nih.gov/pubmed/32484810
http://dx.doi.org/10.1371/journal.pone.0233707
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