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Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients
Royal jelly (RJ), a creamy substance secreted by honeybees, is the exclusive diet for queen bee differentiation and life maintenance. RJ has been used in cosmetics, beverages, medicines, and supplements worldwide. However, allergy is a concerning issue for RJ, especially in atopic dermatitis (AD) an...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266330/ https://www.ncbi.nlm.nih.gov/pubmed/32484810 http://dx.doi.org/10.1371/journal.pone.0233707 |
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author | Hata, Taketoshi Furusawa-Horie, Takako Arai, Yasuko Takahashi, Tomoko Seishima, Mariko Ichihara, Kenji |
author_facet | Hata, Taketoshi Furusawa-Horie, Takako Arai, Yasuko Takahashi, Tomoko Seishima, Mariko Ichihara, Kenji |
author_sort | Hata, Taketoshi |
collection | PubMed |
description | Royal jelly (RJ), a creamy substance secreted by honeybees, is the exclusive diet for queen bee differentiation and life maintenance. RJ has been used in cosmetics, beverages, medicines, and supplements worldwide. However, allergy is a concerning issue for RJ, especially in atopic dermatitis (AD) and asthma patients. In some cases, allergic reactions are seen after the first intake of RJ, suggesting the existence of allergens cross-reactive with RJ. Information about the cross-reactive allergens is very important for the safe application of RJ; however, study of this cross-reactivity is quite limited. In this study, we attempted to identify allergens cross-reactive with RJ by using serum samples from 30 AD patients who had never been exposed to RJ. In an enzyme-linked immunosorbent assay (ELISA) experiment, RJ-binding IgE antibodies were detected in the serum of 10 out of 30 patients, and their antibody titers ranged from 4- to 2,048-fold dilution ratios. Additionally, 3 AD patients were determined to be positive in a skin-prick test (SPT) with an RJ solution. Significant correlations were observed between the anti-RJ antibody titer and nonspecific IgE and between the anti-RJ antibody titer and the Eczema Area and Severity Index score. We further examined the cross-reactivity between RJ and 14 typical allergens by using an ELISA-inhibition assay and demonstrated that the following 6 allergens showed cross-reactivity with RJ: the European house dust mite (HDM) (Dermatophagoides pteronyssinus), American HDM (Dermatophagoides farinae), snow crab (Chionocetes spp.), edible crab (Cancer pagurus), German cockroach (Blatella germanica), and honeybee venom (Apis mellifera). In conclusion, people with a history of allergic diseases, including AD, asthma, and allergic rhinitis, should be cautioned against consuming RJ products because of the potential for cross-reactive responses to ensure the safe and successful use of RJ supplements. |
format | Online Article Text |
id | pubmed-7266330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72663302020-06-10 Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients Hata, Taketoshi Furusawa-Horie, Takako Arai, Yasuko Takahashi, Tomoko Seishima, Mariko Ichihara, Kenji PLoS One Research Article Royal jelly (RJ), a creamy substance secreted by honeybees, is the exclusive diet for queen bee differentiation and life maintenance. RJ has been used in cosmetics, beverages, medicines, and supplements worldwide. However, allergy is a concerning issue for RJ, especially in atopic dermatitis (AD) and asthma patients. In some cases, allergic reactions are seen after the first intake of RJ, suggesting the existence of allergens cross-reactive with RJ. Information about the cross-reactive allergens is very important for the safe application of RJ; however, study of this cross-reactivity is quite limited. In this study, we attempted to identify allergens cross-reactive with RJ by using serum samples from 30 AD patients who had never been exposed to RJ. In an enzyme-linked immunosorbent assay (ELISA) experiment, RJ-binding IgE antibodies were detected in the serum of 10 out of 30 patients, and their antibody titers ranged from 4- to 2,048-fold dilution ratios. Additionally, 3 AD patients were determined to be positive in a skin-prick test (SPT) with an RJ solution. Significant correlations were observed between the anti-RJ antibody titer and nonspecific IgE and between the anti-RJ antibody titer and the Eczema Area and Severity Index score. We further examined the cross-reactivity between RJ and 14 typical allergens by using an ELISA-inhibition assay and demonstrated that the following 6 allergens showed cross-reactivity with RJ: the European house dust mite (HDM) (Dermatophagoides pteronyssinus), American HDM (Dermatophagoides farinae), snow crab (Chionocetes spp.), edible crab (Cancer pagurus), German cockroach (Blatella germanica), and honeybee venom (Apis mellifera). In conclusion, people with a history of allergic diseases, including AD, asthma, and allergic rhinitis, should be cautioned against consuming RJ products because of the potential for cross-reactive responses to ensure the safe and successful use of RJ supplements. Public Library of Science 2020-06-02 /pmc/articles/PMC7266330/ /pubmed/32484810 http://dx.doi.org/10.1371/journal.pone.0233707 Text en © 2020 Hata et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hata, Taketoshi Furusawa-Horie, Takako Arai, Yasuko Takahashi, Tomoko Seishima, Mariko Ichihara, Kenji Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients |
title | Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients |
title_full | Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients |
title_fullStr | Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients |
title_full_unstemmed | Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients |
title_short | Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients |
title_sort | studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266330/ https://www.ncbi.nlm.nih.gov/pubmed/32484810 http://dx.doi.org/10.1371/journal.pone.0233707 |
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