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Dissecting the Polyhydroxyalkanoate-Binding Domain of the PhaF Phasin: Rational Design of a Minimized Affinity Tag

Phasin PhaF from Pseudomonas putida consists of a modular protein whose N-terminal domain (BioF) has been demonstrated to be responsible for binding to the polyhydroxyalkanoate (PHA) granule. BioF has been exploited for biotechnological purposes as an affinity tag in the functionalization of PHA bea...

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Detalles Bibliográficos
Autores principales: Mato, Aranzazu, Blanco, Francisco G., Maestro, Beatriz, Sanz, Jesús M., Pérez-Gil, Jesús, Prieto, M. Auxiliadora
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7267194/
https://www.ncbi.nlm.nih.gov/pubmed/32303541
http://dx.doi.org/10.1128/AEM.00570-20