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Dissecting the Polyhydroxyalkanoate-Binding Domain of the PhaF Phasin: Rational Design of a Minimized Affinity Tag
Phasin PhaF from Pseudomonas putida consists of a modular protein whose N-terminal domain (BioF) has been demonstrated to be responsible for binding to the polyhydroxyalkanoate (PHA) granule. BioF has been exploited for biotechnological purposes as an affinity tag in the functionalization of PHA bea...
Autores principales: | Mato, Aranzazu, Blanco, Francisco G., Maestro, Beatriz, Sanz, Jesús M., Pérez-Gil, Jesús, Prieto, M. Auxiliadora |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7267194/ https://www.ncbi.nlm.nih.gov/pubmed/32303541 http://dx.doi.org/10.1128/AEM.00570-20 |
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