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Industrial enzymes-producing marine bacteria from marine resources

Industrial enzymes are important for various biotechnological applications. Currently, the diversity of industrial enzymes-producing marine bacteria from Malaysia remains mostly unknown. This study investigated the diversity of industrial enzyme-producing marine bacteria from culture collections at...

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Detalles Bibliográficos
Autores principales: Cheng, T.H., Ismail, N., Kamaruding, N., Saidin, J., Danish-Daniel, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7267704/
https://www.ncbi.nlm.nih.gov/pubmed/32514406
http://dx.doi.org/10.1016/j.btre.2020.e00482
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author Cheng, T.H.
Ismail, N.
Kamaruding, N.
Saidin, J.
Danish-Daniel, M.
author_facet Cheng, T.H.
Ismail, N.
Kamaruding, N.
Saidin, J.
Danish-Daniel, M.
author_sort Cheng, T.H.
collection PubMed
description Industrial enzymes are important for various biotechnological applications. Currently, the diversity of industrial enzymes-producing marine bacteria from Malaysia remains mostly unknown. This study investigated the diversity of industrial enzyme-producing marine bacteria from culture collections at the Institute of Marine Biotechnology, Universiti Malaysia Terengganu. Out of 200 bacterial isolates revived, 163 bacteria isolate were successfully growth. Marine bacteria produced enzymes with total scoring higher than four were selected for molecular identification using 16S rDNA. About 161 bacteria isolate secreted amylase (68.7 %), lipase (88.3 %) and protease (68.7 %). The phylogenetic analysis led to the identification of three major phyla, namely Proteobacteria, Firmicutes and Bacteroidetes. These phyla were differentiated into nine genera consisted of Bacillus, Chryseomicrobium, Photobacterium, Pseudoalteromonas, Ruegeria, Shewanella, Solibacillus, Tenacibaculum and Vibrio. Genetic variation was more likely to occur within similar marine bacteria species. The microbial community was found to affect the production of industrial enzymes and the diversity of marine bacteria.
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spelling pubmed-72677042020-06-07 Industrial enzymes-producing marine bacteria from marine resources Cheng, T.H. Ismail, N. Kamaruding, N. Saidin, J. Danish-Daniel, M. Biotechnol Rep (Amst) Research Article Industrial enzymes are important for various biotechnological applications. Currently, the diversity of industrial enzymes-producing marine bacteria from Malaysia remains mostly unknown. This study investigated the diversity of industrial enzyme-producing marine bacteria from culture collections at the Institute of Marine Biotechnology, Universiti Malaysia Terengganu. Out of 200 bacterial isolates revived, 163 bacteria isolate were successfully growth. Marine bacteria produced enzymes with total scoring higher than four were selected for molecular identification using 16S rDNA. About 161 bacteria isolate secreted amylase (68.7 %), lipase (88.3 %) and protease (68.7 %). The phylogenetic analysis led to the identification of three major phyla, namely Proteobacteria, Firmicutes and Bacteroidetes. These phyla were differentiated into nine genera consisted of Bacillus, Chryseomicrobium, Photobacterium, Pseudoalteromonas, Ruegeria, Shewanella, Solibacillus, Tenacibaculum and Vibrio. Genetic variation was more likely to occur within similar marine bacteria species. The microbial community was found to affect the production of industrial enzymes and the diversity of marine bacteria. Elsevier 2020-05-29 /pmc/articles/PMC7267704/ /pubmed/32514406 http://dx.doi.org/10.1016/j.btre.2020.e00482 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Cheng, T.H.
Ismail, N.
Kamaruding, N.
Saidin, J.
Danish-Daniel, M.
Industrial enzymes-producing marine bacteria from marine resources
title Industrial enzymes-producing marine bacteria from marine resources
title_full Industrial enzymes-producing marine bacteria from marine resources
title_fullStr Industrial enzymes-producing marine bacteria from marine resources
title_full_unstemmed Industrial enzymes-producing marine bacteria from marine resources
title_short Industrial enzymes-producing marine bacteria from marine resources
title_sort industrial enzymes-producing marine bacteria from marine resources
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7267704/
https://www.ncbi.nlm.nih.gov/pubmed/32514406
http://dx.doi.org/10.1016/j.btre.2020.e00482
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