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Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa

Peptidoglycan (PG) is an essential component of the bacterial cell wall and is assembled from a lipid II precursor by glycosyltransferase and transpeptidase reactions catalyzed in particular by bifunctional class A penicillin-binding proteins (aPBPs). In the major clinical pathogen Pseudomonas aerug...

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Autores principales: Caveney, Nathanael A., Egan, Alexander J.F., Ayala, Isabel, Laguri, Cédric, Robb, Craig S., Breukink, Eefjan, Vollmer, Waldemar, Strynadka, Natalie C.J., Simorre, Jean-Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7267771/
https://www.ncbi.nlm.nih.gov/pubmed/32320673
http://dx.doi.org/10.1016/j.str.2020.03.012
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author Caveney, Nathanael A.
Egan, Alexander J.F.
Ayala, Isabel
Laguri, Cédric
Robb, Craig S.
Breukink, Eefjan
Vollmer, Waldemar
Strynadka, Natalie C.J.
Simorre, Jean-Pierre
author_facet Caveney, Nathanael A.
Egan, Alexander J.F.
Ayala, Isabel
Laguri, Cédric
Robb, Craig S.
Breukink, Eefjan
Vollmer, Waldemar
Strynadka, Natalie C.J.
Simorre, Jean-Pierre
author_sort Caveney, Nathanael A.
collection PubMed
description Peptidoglycan (PG) is an essential component of the bacterial cell wall and is assembled from a lipid II precursor by glycosyltransferase and transpeptidase reactions catalyzed in particular by bifunctional class A penicillin-binding proteins (aPBPs). In the major clinical pathogen Pseudomonas aeruginosa, PBP1B is anchored within the cytoplasmic membrane but regulated by a bespoke outer membrane-localized lipoprotein known as LpoP. Here, we report the structure of LpoP, showing an extended N-terminal, flexible tether followed by a well-ordered C-terminal tandem-tetratricopeptide repeat domain. We show that LpoP stimulates both PBP1B transpeptidase and glycosyltransferase activities in vitro and interacts directly via its C terminus globular domain with the central UB2H domain of PBP1B. Contrary to the situation in E. coli, P. aeruginosa CpoB does not regulate PBP1B/LpoP in vitro. We propose a mechanism that helps to underscore similarities and differences in class A PBP activation across Gram-negative bacteria.
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spelling pubmed-72677712020-06-08 Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa Caveney, Nathanael A. Egan, Alexander J.F. Ayala, Isabel Laguri, Cédric Robb, Craig S. Breukink, Eefjan Vollmer, Waldemar Strynadka, Natalie C.J. Simorre, Jean-Pierre Structure Article Peptidoglycan (PG) is an essential component of the bacterial cell wall and is assembled from a lipid II precursor by glycosyltransferase and transpeptidase reactions catalyzed in particular by bifunctional class A penicillin-binding proteins (aPBPs). In the major clinical pathogen Pseudomonas aeruginosa, PBP1B is anchored within the cytoplasmic membrane but regulated by a bespoke outer membrane-localized lipoprotein known as LpoP. Here, we report the structure of LpoP, showing an extended N-terminal, flexible tether followed by a well-ordered C-terminal tandem-tetratricopeptide repeat domain. We show that LpoP stimulates both PBP1B transpeptidase and glycosyltransferase activities in vitro and interacts directly via its C terminus globular domain with the central UB2H domain of PBP1B. Contrary to the situation in E. coli, P. aeruginosa CpoB does not regulate PBP1B/LpoP in vitro. We propose a mechanism that helps to underscore similarities and differences in class A PBP activation across Gram-negative bacteria. Cell Press 2020-06-02 /pmc/articles/PMC7267771/ /pubmed/32320673 http://dx.doi.org/10.1016/j.str.2020.03.012 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Caveney, Nathanael A.
Egan, Alexander J.F.
Ayala, Isabel
Laguri, Cédric
Robb, Craig S.
Breukink, Eefjan
Vollmer, Waldemar
Strynadka, Natalie C.J.
Simorre, Jean-Pierre
Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa
title Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa
title_full Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa
title_fullStr Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa
title_full_unstemmed Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa
title_short Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa
title_sort structure of the peptidoglycan synthase activator lpop in pseudomonas aeruginosa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7267771/
https://www.ncbi.nlm.nih.gov/pubmed/32320673
http://dx.doi.org/10.1016/j.str.2020.03.012
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