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Uptake and Retention of Nanoplastics in Quagga Mussels

Here, a set of experiments to assess the feasibility of using an invasive and widespread freshwater mussel (Dreissena rostrformis bugensis) as a sentinel species for nanoplastic detection is reported. Under laboratory experimental conditions, mussels ingest and retain fluorescent polystyrene (PS) be...

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Autores principales: Merzel, Rachel L., Purser, Lauren, Soucy, Taylor L., Olszewski, Monica, Colón‐Bernal, Isabel, Duhaime, Melissa, Elgin, Ashley K., Banaszak Holl, Mark M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268195/
https://www.ncbi.nlm.nih.gov/pubmed/32685193
http://dx.doi.org/10.1002/gch2.201800104
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author Merzel, Rachel L.
Purser, Lauren
Soucy, Taylor L.
Olszewski, Monica
Colón‐Bernal, Isabel
Duhaime, Melissa
Elgin, Ashley K.
Banaszak Holl, Mark M.
author_facet Merzel, Rachel L.
Purser, Lauren
Soucy, Taylor L.
Olszewski, Monica
Colón‐Bernal, Isabel
Duhaime, Melissa
Elgin, Ashley K.
Banaszak Holl, Mark M.
author_sort Merzel, Rachel L.
collection PubMed
description Here, a set of experiments to assess the feasibility of using an invasive and widespread freshwater mussel (Dreissena rostrformis bugensis) as a sentinel species for nanoplastic detection is reported. Under laboratory experimental conditions, mussels ingest and retain fluorescent polystyrene (PS) beads with carboxylic acid (—COOH) termination over a size range of 200–2000 nm. The number of beads the mussels ingested is quantified using fluorescence spectroscopy and the location of the beads in the mussels is imaged using fluorescence microscopy. PS beads of similar size (1000–2000 nm) to mussels' preferred food are trafficked in the ciliated food grooves of the gills. Beads of all sizes are observed in the mussels' digestive tracts, indicating that the mussels do not efficiently reject the beads as unwanted foreign material, regardless of size. Fluorescence microscopy shows all sizes of beads are concentrated in the siphons and are retained there for longer than one month postexposure. Combined atomic force microscopy–infrared spectroscopy and photothermal infrared spectroscopy are used to locate, image, and chemically identify the beads in the mussel siphons. In sum, these experiments demonstrate the potential for using mussels, specifically their siphons, to monitor environmental accumulation of aquatic nanoplastics.
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spelling pubmed-72681952020-07-17 Uptake and Retention of Nanoplastics in Quagga Mussels Merzel, Rachel L. Purser, Lauren Soucy, Taylor L. Olszewski, Monica Colón‐Bernal, Isabel Duhaime, Melissa Elgin, Ashley K. Banaszak Holl, Mark M. Glob Chall Full Papers Here, a set of experiments to assess the feasibility of using an invasive and widespread freshwater mussel (Dreissena rostrformis bugensis) as a sentinel species for nanoplastic detection is reported. Under laboratory experimental conditions, mussels ingest and retain fluorescent polystyrene (PS) beads with carboxylic acid (—COOH) termination over a size range of 200–2000 nm. The number of beads the mussels ingested is quantified using fluorescence spectroscopy and the location of the beads in the mussels is imaged using fluorescence microscopy. PS beads of similar size (1000–2000 nm) to mussels' preferred food are trafficked in the ciliated food grooves of the gills. Beads of all sizes are observed in the mussels' digestive tracts, indicating that the mussels do not efficiently reject the beads as unwanted foreign material, regardless of size. Fluorescence microscopy shows all sizes of beads are concentrated in the siphons and are retained there for longer than one month postexposure. Combined atomic force microscopy–infrared spectroscopy and photothermal infrared spectroscopy are used to locate, image, and chemically identify the beads in the mussel siphons. In sum, these experiments demonstrate the potential for using mussels, specifically their siphons, to monitor environmental accumulation of aquatic nanoplastics. John Wiley and Sons Inc. 2019-07-02 /pmc/articles/PMC7268195/ /pubmed/32685193 http://dx.doi.org/10.1002/gch2.201800104 Text en © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Merzel, Rachel L.
Purser, Lauren
Soucy, Taylor L.
Olszewski, Monica
Colón‐Bernal, Isabel
Duhaime, Melissa
Elgin, Ashley K.
Banaszak Holl, Mark M.
Uptake and Retention of Nanoplastics in Quagga Mussels
title Uptake and Retention of Nanoplastics in Quagga Mussels
title_full Uptake and Retention of Nanoplastics in Quagga Mussels
title_fullStr Uptake and Retention of Nanoplastics in Quagga Mussels
title_full_unstemmed Uptake and Retention of Nanoplastics in Quagga Mussels
title_short Uptake and Retention of Nanoplastics in Quagga Mussels
title_sort uptake and retention of nanoplastics in quagga mussels
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268195/
https://www.ncbi.nlm.nih.gov/pubmed/32685193
http://dx.doi.org/10.1002/gch2.201800104
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