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A transient CRISPR/Cas9 expression system for genome editing in Trypanosoma brucei

OBJECTIVE: Generation of knockouts and in situ tagging of genes in Trypanosoma brucei has been greatly facilitated by using CRISPR/Cas9 as a genome editing tool. To date, this has entailed using a limited number of cell lines that are stably transformed to express Cas9 and T7 RNA polymerase (T7RNAP)...

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Detalles Bibliográficos
Autores principales:	Shaw, Sebastian, Knüsel, Sebastian, Hoenner, Sarah, Roditi, Isabel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Research Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268226/ https://www.ncbi.nlm.nih.gov/pubmed/32493474 http://dx.doi.org/10.1186/s13104-020-05089-z

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