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Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila
BACKGROUND: Aeromonas hydrophila is an important water-borne pathogen that leads to a great economic loss in aquaculture. Along with the abuse of antibiotics, drug-resistant strains rise rapidly. In addition, the biofilms formed by this bacterium limited the antibacterial effect of antibiotics. Bact...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268745/ https://www.ncbi.nlm.nih.gov/pubmed/32487015 http://dx.doi.org/10.1186/s12866-020-01811-w |
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author | Liu, Jin Gao, Shanshan Dong, Yuhao Lu, Chengping Liu, Yongjie |
author_facet | Liu, Jin Gao, Shanshan Dong, Yuhao Lu, Chengping Liu, Yongjie |
author_sort | Liu, Jin |
collection | PubMed |
description | BACKGROUND: Aeromonas hydrophila is an important water-borne pathogen that leads to a great economic loss in aquaculture. Along with the abuse of antibiotics, drug-resistant strains rise rapidly. In addition, the biofilms formed by this bacterium limited the antibacterial effect of antibiotics. Bacteriophages have been attracting increasing attention as a potential alternative to antibiotics against bacterial infections. RESULTS: Five phages against pathogenic A. hydrophila, named N21, W3, G65, Y71 and Y81, were isolated. Morphological analysis by transmission electron microscopy revealed that phages N21, W3 and G65 belong to the family Myoviridae, while Y71 and Y81 belong to the Podoviridae. These phages were found to have broad host spectra, short latent periods and normal burst sizes. They were sensitive to high temperature but had a wide adaptability to the pH. In addition, the phages G65 and Y81 showed considerable bacterial killing effect and potential in preventing formation of A. hydrophila biofilm; and the phages G65, W3 and N21 were able to scavenge mature biofilm effectively. Phage treatments applied to the pathogenic A. hydrophila in mice model resulted in a significantly decreased bacterial loads in tissues. CONCLUSIONS: Five A. hydrophila phages were isolated with broad host ranges, low latent periods, and wide pH and thermal tolerance. And the phages exhibited varying abilities in controlling A. hydrophila infection. This work presents promising data supporting the future use of phage therapy. |
format | Online Article Text |
id | pubmed-7268745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-72687452020-06-08 Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila Liu, Jin Gao, Shanshan Dong, Yuhao Lu, Chengping Liu, Yongjie BMC Microbiol Research Article BACKGROUND: Aeromonas hydrophila is an important water-borne pathogen that leads to a great economic loss in aquaculture. Along with the abuse of antibiotics, drug-resistant strains rise rapidly. In addition, the biofilms formed by this bacterium limited the antibacterial effect of antibiotics. Bacteriophages have been attracting increasing attention as a potential alternative to antibiotics against bacterial infections. RESULTS: Five phages against pathogenic A. hydrophila, named N21, W3, G65, Y71 and Y81, were isolated. Morphological analysis by transmission electron microscopy revealed that phages N21, W3 and G65 belong to the family Myoviridae, while Y71 and Y81 belong to the Podoviridae. These phages were found to have broad host spectra, short latent periods and normal burst sizes. They were sensitive to high temperature but had a wide adaptability to the pH. In addition, the phages G65 and Y81 showed considerable bacterial killing effect and potential in preventing formation of A. hydrophila biofilm; and the phages G65, W3 and N21 were able to scavenge mature biofilm effectively. Phage treatments applied to the pathogenic A. hydrophila in mice model resulted in a significantly decreased bacterial loads in tissues. CONCLUSIONS: Five A. hydrophila phages were isolated with broad host ranges, low latent periods, and wide pH and thermal tolerance. And the phages exhibited varying abilities in controlling A. hydrophila infection. This work presents promising data supporting the future use of phage therapy. BioMed Central 2020-06-01 /pmc/articles/PMC7268745/ /pubmed/32487015 http://dx.doi.org/10.1186/s12866-020-01811-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Liu, Jin Gao, Shanshan Dong, Yuhao Lu, Chengping Liu, Yongjie Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila |
title | Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila |
title_full | Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila |
title_fullStr | Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila |
title_full_unstemmed | Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila |
title_short | Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila |
title_sort | isolation and characterization of bacteriophages against virulent aeromonas hydrophila |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268745/ https://www.ncbi.nlm.nih.gov/pubmed/32487015 http://dx.doi.org/10.1186/s12866-020-01811-w |
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