Cargando…
Study of Hypoxia-inducible factor-2α expression in the malignant transformation of Oral submucous fibrosis
CONTEXT: Hypoxia-inducible factor (HIF)-2α is overexpressed in primary and metastatic human cancers, whose expression is correlated with tumor angiogenesis and patient mortality. HIF plays a role in the progression of fibrosis in oral submucous fibrosis (OSF). AIM AND OBJECTIVE: The aim is to study...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7269270/ https://www.ncbi.nlm.nih.gov/pubmed/32508445 http://dx.doi.org/10.4103/jomfp.JOMFP_42_19 |
_version_ | 1783541746884411392 |
---|---|
author | Joseph, Immanuel Elizabeth, Joshua Rao, Umadevi K Ranganathan, Kannan |
author_facet | Joseph, Immanuel Elizabeth, Joshua Rao, Umadevi K Ranganathan, Kannan |
author_sort | Joseph, Immanuel |
collection | PubMed |
description | CONTEXT: Hypoxia-inducible factor (HIF)-2α is overexpressed in primary and metastatic human cancers, whose expression is correlated with tumor angiogenesis and patient mortality. HIF plays a role in the progression of fibrosis in oral submucous fibrosis (OSF). AIM AND OBJECTIVE: The aim is to study and compare the expression of HIF-2α in OSF (a), oral squamous cell carcinoma (OSCC) with areca nut usage (b), OSCC without areca nut usage (c) and normal mucosa (d) by immunohistochemistry. SUBJECTS AND METHODS: Immunohistochemical detection of HIF-2α was done on 51 samples, which included 11 cases (a), 15 cases (b), 15 cases (c) and the expression was compared with that of (d). STATISTICAL ANALYSIS: Data were analyzed using the SPSS™ software (ver. 21.0). Chi-square test and kappa analysis were performed to compare the intensity of staining between the groups and for inter-observer agreement, respectively. Value of P ≤ 0.05 was considered statistically significant. The mean labeling index between the groups was studied by the Kruskal–Wallis test. RESULTS: All the cases of (d), (a), (b) and (c) showed HIF-2α expression (P = 0.329). About 13% cases of (c) showed intense expression (P = 0.406) and 27% of (a) cases showed expression only in the connective tissue (P = 0.023). The number of positively stained nuclei in both (b and c) cases reduced as the tumor progression was from well to poorly differentiated. CONCLUSION: Areca nut initiates fibrosis and subsequent hypoxia in OSF which triggers HIF-2α expression in the epithelium. HIF-2α could be a surrogate marker for cancer initiation and progression. |
format | Online Article Text |
id | pubmed-7269270 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-72692702020-06-05 Study of Hypoxia-inducible factor-2α expression in the malignant transformation of Oral submucous fibrosis Joseph, Immanuel Elizabeth, Joshua Rao, Umadevi K Ranganathan, Kannan J Oral Maxillofac Pathol Original Article CONTEXT: Hypoxia-inducible factor (HIF)-2α is overexpressed in primary and metastatic human cancers, whose expression is correlated with tumor angiogenesis and patient mortality. HIF plays a role in the progression of fibrosis in oral submucous fibrosis (OSF). AIM AND OBJECTIVE: The aim is to study and compare the expression of HIF-2α in OSF (a), oral squamous cell carcinoma (OSCC) with areca nut usage (b), OSCC without areca nut usage (c) and normal mucosa (d) by immunohistochemistry. SUBJECTS AND METHODS: Immunohistochemical detection of HIF-2α was done on 51 samples, which included 11 cases (a), 15 cases (b), 15 cases (c) and the expression was compared with that of (d). STATISTICAL ANALYSIS: Data were analyzed using the SPSS™ software (ver. 21.0). Chi-square test and kappa analysis were performed to compare the intensity of staining between the groups and for inter-observer agreement, respectively. Value of P ≤ 0.05 was considered statistically significant. The mean labeling index between the groups was studied by the Kruskal–Wallis test. RESULTS: All the cases of (d), (a), (b) and (c) showed HIF-2α expression (P = 0.329). About 13% cases of (c) showed intense expression (P = 0.406) and 27% of (a) cases showed expression only in the connective tissue (P = 0.023). The number of positively stained nuclei in both (b and c) cases reduced as the tumor progression was from well to poorly differentiated. CONCLUSION: Areca nut initiates fibrosis and subsequent hypoxia in OSF which triggers HIF-2α expression in the epithelium. HIF-2α could be a surrogate marker for cancer initiation and progression. Wolters Kluwer - Medknow 2020 2020-05-08 /pmc/articles/PMC7269270/ /pubmed/32508445 http://dx.doi.org/10.4103/jomfp.JOMFP_42_19 Text en Copyright: © 2020 Journal of Oral and Maxillofacial Pathology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Joseph, Immanuel Elizabeth, Joshua Rao, Umadevi K Ranganathan, Kannan Study of Hypoxia-inducible factor-2α expression in the malignant transformation of Oral submucous fibrosis |
title | Study of Hypoxia-inducible factor-2α expression in the malignant transformation of Oral submucous fibrosis |
title_full | Study of Hypoxia-inducible factor-2α expression in the malignant transformation of Oral submucous fibrosis |
title_fullStr | Study of Hypoxia-inducible factor-2α expression in the malignant transformation of Oral submucous fibrosis |
title_full_unstemmed | Study of Hypoxia-inducible factor-2α expression in the malignant transformation of Oral submucous fibrosis |
title_short | Study of Hypoxia-inducible factor-2α expression in the malignant transformation of Oral submucous fibrosis |
title_sort | study of hypoxia-inducible factor-2α expression in the malignant transformation of oral submucous fibrosis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7269270/ https://www.ncbi.nlm.nih.gov/pubmed/32508445 http://dx.doi.org/10.4103/jomfp.JOMFP_42_19 |
work_keys_str_mv | AT josephimmanuel studyofhypoxiainduciblefactor2aexpressioninthemalignanttransformationoforalsubmucousfibrosis AT elizabethjoshua studyofhypoxiainduciblefactor2aexpressioninthemalignanttransformationoforalsubmucousfibrosis AT raoumadevik studyofhypoxiainduciblefactor2aexpressioninthemalignanttransformationoforalsubmucousfibrosis AT ranganathankannan studyofhypoxiainduciblefactor2aexpressioninthemalignanttransformationoforalsubmucousfibrosis |