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Tissue engineered endometrial barrier exposed to peristaltic flow shear stresses

Cyclic myometrial contractions of the non-pregnant uterus induce intra-uterine peristaltic flows, which have important roles in transport of sperm and embryos during early stages of reproduction. Hyperperistalsis in young females may lead to migration of endometrial cells and development of adenomyo...

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Autores principales: Elad, David, Zaretsky, Uri, Kuperman, Tatyana, Gavriel, Mark, Long, Mian, Jaffa, Ariel, Grisaru, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7269682/
https://www.ncbi.nlm.nih.gov/pubmed/32548541
http://dx.doi.org/10.1063/5.0001994
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author Elad, David
Zaretsky, Uri
Kuperman, Tatyana
Gavriel, Mark
Long, Mian
Jaffa, Ariel
Grisaru, Dan
author_facet Elad, David
Zaretsky, Uri
Kuperman, Tatyana
Gavriel, Mark
Long, Mian
Jaffa, Ariel
Grisaru, Dan
author_sort Elad, David
collection PubMed
description Cyclic myometrial contractions of the non-pregnant uterus induce intra-uterine peristaltic flows, which have important roles in transport of sperm and embryos during early stages of reproduction. Hyperperistalsis in young females may lead to migration of endometrial cells and development of adenomyosis or endometriosis. We conducted an in vitro study of the biological response of a tissue engineered endometrial barrier exposed to peristaltic wall shear stresses (PWSSs). The endometrial barrier model was co-cultured of endometrial epithelial cells on top of myometrial smooth muscle cells (MSMCs) in custom-designed wells that can be disassembled for mechanobiology experiments. A new experimental setup was developed for exposing the uterine wall in vitro model to PWSSs that mimic the in vivo intra-uterine environment. Peristaltic flow was induced by moving a belt with bulges to deform the elastic cover of a fluid filled chamber that held the uterine wall model at the bottom. The in vitro biological model was exposed to peristaltic flows for 60 and 120 min and then stained for immunofluorescence studies of alternations in the cytoskeleton. Quantification of the F-actin mass in both layers revealed a significant increase with the length of exposure to PWSSs. Moreover, the inner layer of MSMCs that were not in direct contact with the fluid also responded with an increase in the F-actin mass. This new experimental approach can be expanded to in vitro studies of multiple structural changes and genetic expressions, while the tissue engineered uterine wall models are tested under conditions that mimic the in vivo physiological environment.
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spelling pubmed-72696822020-06-15 Tissue engineered endometrial barrier exposed to peristaltic flow shear stresses Elad, David Zaretsky, Uri Kuperman, Tatyana Gavriel, Mark Long, Mian Jaffa, Ariel Grisaru, Dan APL Bioeng Articles Cyclic myometrial contractions of the non-pregnant uterus induce intra-uterine peristaltic flows, which have important roles in transport of sperm and embryos during early stages of reproduction. Hyperperistalsis in young females may lead to migration of endometrial cells and development of adenomyosis or endometriosis. We conducted an in vitro study of the biological response of a tissue engineered endometrial barrier exposed to peristaltic wall shear stresses (PWSSs). The endometrial barrier model was co-cultured of endometrial epithelial cells on top of myometrial smooth muscle cells (MSMCs) in custom-designed wells that can be disassembled for mechanobiology experiments. A new experimental setup was developed for exposing the uterine wall in vitro model to PWSSs that mimic the in vivo intra-uterine environment. Peristaltic flow was induced by moving a belt with bulges to deform the elastic cover of a fluid filled chamber that held the uterine wall model at the bottom. The in vitro biological model was exposed to peristaltic flows for 60 and 120 min and then stained for immunofluorescence studies of alternations in the cytoskeleton. Quantification of the F-actin mass in both layers revealed a significant increase with the length of exposure to PWSSs. Moreover, the inner layer of MSMCs that were not in direct contact with the fluid also responded with an increase in the F-actin mass. This new experimental approach can be expanded to in vitro studies of multiple structural changes and genetic expressions, while the tissue engineered uterine wall models are tested under conditions that mimic the in vivo physiological environment. AIP Publishing LLC 2020-06-02 /pmc/articles/PMC7269682/ /pubmed/32548541 http://dx.doi.org/10.1063/5.0001994 Text en © 2020 Author(s). 2473-2877/2020/4(2)/026107/11 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Articles
Elad, David
Zaretsky, Uri
Kuperman, Tatyana
Gavriel, Mark
Long, Mian
Jaffa, Ariel
Grisaru, Dan
Tissue engineered endometrial barrier exposed to peristaltic flow shear stresses
title Tissue engineered endometrial barrier exposed to peristaltic flow shear stresses
title_full Tissue engineered endometrial barrier exposed to peristaltic flow shear stresses
title_fullStr Tissue engineered endometrial barrier exposed to peristaltic flow shear stresses
title_full_unstemmed Tissue engineered endometrial barrier exposed to peristaltic flow shear stresses
title_short Tissue engineered endometrial barrier exposed to peristaltic flow shear stresses
title_sort tissue engineered endometrial barrier exposed to peristaltic flow shear stresses
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7269682/
https://www.ncbi.nlm.nih.gov/pubmed/32548541
http://dx.doi.org/10.1063/5.0001994
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