Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe

[Image: see text] Nucleic acid tests integrated into digital point-of-care (POC) diagnostic systems have great potential for the future of health care. However, current methods of DNA amplification and detection require bulky and expensive equipment, many steps, and long process times, which complic...

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Autores principales: Khaliliazar, Shirin, Ouyang, Liangqi, Piper, Andrew, Chondrogiannis, Georgios, Hanze, Martin, Herland, Anna, Hamedi, Mahiar Max
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271026/
https://www.ncbi.nlm.nih.gov/pubmed/32548389
http://dx.doi.org/10.1021/acsomega.0c00341
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author Khaliliazar, Shirin
Ouyang, Liangqi
Piper, Andrew
Chondrogiannis, Georgios
Hanze, Martin
Herland, Anna
Hamedi, Mahiar Max
author_facet Khaliliazar, Shirin
Ouyang, Liangqi
Piper, Andrew
Chondrogiannis, Georgios
Hanze, Martin
Herland, Anna
Hamedi, Mahiar Max
author_sort Khaliliazar, Shirin
collection PubMed
description [Image: see text] Nucleic acid tests integrated into digital point-of-care (POC) diagnostic systems have great potential for the future of health care. However, current methods of DNA amplification and detection require bulky and expensive equipment, many steps, and long process times, which complicate their integration into POC devices. We have combined an isothermal DNA amplification method, recombinase polymerase amplification, with an electrochemical stem-loop (S-L) probe DNA detection technique. By combining these methods, we have created a system that is able to specifically amplify and detect as few as 10 copies/μL Staphylococcus epidermidis DNA with a total time to result of 70−75 min.
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spelling pubmed-72710262020-06-15 Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe Khaliliazar, Shirin Ouyang, Liangqi Piper, Andrew Chondrogiannis, Georgios Hanze, Martin Herland, Anna Hamedi, Mahiar Max ACS Omega [Image: see text] Nucleic acid tests integrated into digital point-of-care (POC) diagnostic systems have great potential for the future of health care. However, current methods of DNA amplification and detection require bulky and expensive equipment, many steps, and long process times, which complicate their integration into POC devices. We have combined an isothermal DNA amplification method, recombinase polymerase amplification, with an electrochemical stem-loop (S-L) probe DNA detection technique. By combining these methods, we have created a system that is able to specifically amplify and detect as few as 10 copies/μL Staphylococcus epidermidis DNA with a total time to result of 70−75 min. American Chemical Society 2020-05-20 /pmc/articles/PMC7271026/ /pubmed/32548389 http://dx.doi.org/10.1021/acsomega.0c00341 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Khaliliazar, Shirin
Ouyang, Liangqi
Piper, Andrew
Chondrogiannis, Georgios
Hanze, Martin
Herland, Anna
Hamedi, Mahiar Max
Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe
title Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe
title_full Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe
title_fullStr Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe
title_full_unstemmed Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe
title_short Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe
title_sort electrochemical detection of genomic dna utilizing recombinase polymerase amplification and stem-loop probe
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271026/
https://www.ncbi.nlm.nih.gov/pubmed/32548389
http://dx.doi.org/10.1021/acsomega.0c00341
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