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Paper-Based Platform with an In Situ Molecularly Imprinted Polymer for β-Amyloid

[Image: see text] Alzheimer’s disease (AD) is one of the most common forms of dementia affecting millions of people worldwide. Currently, an easy and effective form of diagnosis is missing, which significantly hinders a possible improvement of the patient’s quality of life. In this context, biosenso...

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Autores principales: Pereira, Marta V., Marques, Ana C., Oliveira, Daniela, Martins, Rodrigo, Moreira, Felismina T. C., Sales, M. Goreti F., Fortunato, Elvira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271027/
https://www.ncbi.nlm.nih.gov/pubmed/32548384
http://dx.doi.org/10.1021/acsomega.0c00062
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author Pereira, Marta V.
Marques, Ana C.
Oliveira, Daniela
Martins, Rodrigo
Moreira, Felismina T. C.
Sales, M. Goreti F.
Fortunato, Elvira
author_facet Pereira, Marta V.
Marques, Ana C.
Oliveira, Daniela
Martins, Rodrigo
Moreira, Felismina T. C.
Sales, M. Goreti F.
Fortunato, Elvira
author_sort Pereira, Marta V.
collection PubMed
description [Image: see text] Alzheimer’s disease (AD) is one of the most common forms of dementia affecting millions of people worldwide. Currently, an easy and effective form of diagnosis is missing, which significantly hinders a possible improvement of the patient’s quality of life. In this context, biosensors emerge as a future solution, opening the doors for preventive medicine and allowing the premature diagnosis of numerous pathologies. This work presents a pioneering biosensor that combines a bottom-up design approach using paper as a platform for the electrochemical recognition of peptide amyloid β-42 (Aβ-42), a biomarker for AD present in blood, associated with visible differences in the brain tissue and responsible for the formation of senile plaques. The sensor layer relies on a molecularly imprinted polymer as a biorecognition element, created on the carbon ink electrode’s surface by electropolymerizing a mixture of the target analyte (Aβ-42) and a monomer (O-phenylenediamine) at neutral pH 7.2. Next, the template molecule was removed from the polymeric network by enzymatic and acidic treatments. The vacant sites so obtained preserved the shape of the imprinted protein and were able to rebind the target analyte. Morphological and chemical analyses were performed in order to control the surface modification of the materials. The analytical performance of the biosensor was evaluated by an electroanalytical technique, namely, square wave voltammetry. For this purpose, the analytical response of the biosensor was tested with standard solutions ranging from 0.1 ng/mL to 1 μg/mL of Aβ-42. The linear response of the biosensor went down to 0.1 ng/mL. Overall, the developed biosensor offered numerous benefits, such as simplicity, low cost, reproducibility, fast response, and repeatability less than 10%. All together, these features may have a strong impact in the early detection of AD.
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spelling pubmed-72710272020-06-15 Paper-Based Platform with an In Situ Molecularly Imprinted Polymer for β-Amyloid Pereira, Marta V. Marques, Ana C. Oliveira, Daniela Martins, Rodrigo Moreira, Felismina T. C. Sales, M. Goreti F. Fortunato, Elvira ACS Omega [Image: see text] Alzheimer’s disease (AD) is one of the most common forms of dementia affecting millions of people worldwide. Currently, an easy and effective form of diagnosis is missing, which significantly hinders a possible improvement of the patient’s quality of life. In this context, biosensors emerge as a future solution, opening the doors for preventive medicine and allowing the premature diagnosis of numerous pathologies. This work presents a pioneering biosensor that combines a bottom-up design approach using paper as a platform for the electrochemical recognition of peptide amyloid β-42 (Aβ-42), a biomarker for AD present in blood, associated with visible differences in the brain tissue and responsible for the formation of senile plaques. The sensor layer relies on a molecularly imprinted polymer as a biorecognition element, created on the carbon ink electrode’s surface by electropolymerizing a mixture of the target analyte (Aβ-42) and a monomer (O-phenylenediamine) at neutral pH 7.2. Next, the template molecule was removed from the polymeric network by enzymatic and acidic treatments. The vacant sites so obtained preserved the shape of the imprinted protein and were able to rebind the target analyte. Morphological and chemical analyses were performed in order to control the surface modification of the materials. The analytical performance of the biosensor was evaluated by an electroanalytical technique, namely, square wave voltammetry. For this purpose, the analytical response of the biosensor was tested with standard solutions ranging from 0.1 ng/mL to 1 μg/mL of Aβ-42. The linear response of the biosensor went down to 0.1 ng/mL. Overall, the developed biosensor offered numerous benefits, such as simplicity, low cost, reproducibility, fast response, and repeatability less than 10%. All together, these features may have a strong impact in the early detection of AD. American Chemical Society 2020-05-15 /pmc/articles/PMC7271027/ /pubmed/32548384 http://dx.doi.org/10.1021/acsomega.0c00062 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Pereira, Marta V.
Marques, Ana C.
Oliveira, Daniela
Martins, Rodrigo
Moreira, Felismina T. C.
Sales, M. Goreti F.
Fortunato, Elvira
Paper-Based Platform with an In Situ Molecularly Imprinted Polymer for β-Amyloid
title Paper-Based Platform with an In Situ Molecularly Imprinted Polymer for β-Amyloid
title_full Paper-Based Platform with an In Situ Molecularly Imprinted Polymer for β-Amyloid
title_fullStr Paper-Based Platform with an In Situ Molecularly Imprinted Polymer for β-Amyloid
title_full_unstemmed Paper-Based Platform with an In Situ Molecularly Imprinted Polymer for β-Amyloid
title_short Paper-Based Platform with an In Situ Molecularly Imprinted Polymer for β-Amyloid
title_sort paper-based platform with an in situ molecularly imprinted polymer for β-amyloid
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271027/
https://www.ncbi.nlm.nih.gov/pubmed/32548384
http://dx.doi.org/10.1021/acsomega.0c00062
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