Propagation of Adenoviral Vectors: Use of PER. C6 Cells

This chapter discusses the production systems of adenoviral vectors. Particular attention is paid to the generation and use of complementation cell lines that carry the El genes and emphasizes on the PER.C6 cell line, which was developed to prevent generation of replication-competent adenovirus (RCA...

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Autores principales: Nichols, W.W., Lardenoije, R., Ledwith, B.J., Brouwer, K., Manam, S., Vogels, R., Kaslow, D., Zuidgeest, D., Bett, A.J., Chen, L., van der Kaaden, M., Galloway, S.M., Hill, R.B., Machotka, S.V., Anderson, C.A., Lewis, J., Martinez, D., Lebron, J., Russo, C., Valerio, D., Bout, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2002
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271171/
http://dx.doi.org/10.1016/B978-012199504-1/50007-9
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author Nichols, W.W.
Lardenoije, R.
Ledwith, B.J.
Brouwer, K.
Manam, S.
Vogels, R.
Kaslow, D.
Zuidgeest, D.
Bett, A.J.
Chen, L.
van der Kaaden, M.
Galloway, S.M.
Hill, R.B.
Machotka, S.V.
Anderson, C.A.
Lewis, J.
Martinez, D.
Lebron, J.
Russo, C.
Valerio, D.
Bout, A.
author_facet Nichols, W.W.
Lardenoije, R.
Ledwith, B.J.
Brouwer, K.
Manam, S.
Vogels, R.
Kaslow, D.
Zuidgeest, D.
Bett, A.J.
Chen, L.
van der Kaaden, M.
Galloway, S.M.
Hill, R.B.
Machotka, S.V.
Anderson, C.A.
Lewis, J.
Martinez, D.
Lebron, J.
Russo, C.
Valerio, D.
Bout, A.
author_sort Nichols, W.W.
collection PubMed
description This chapter discusses the production systems of adenoviral vectors. Particular attention is paid to the generation and use of complementation cell lines that carry the El genes and emphasizes on the PER.C6 cell line, which was developed to prevent generation of replication-competent adenovirus (RCA) during propagation of El-deleted adenoviral vectors. Further, safety issues with respect to the use of the cell line for making clinical grade material are also addressed in this chapter. The PER.C6 cell line is the best substrate for the production of adenoviral vectors for gene therapy or vaccines. PER.C6 permits extensive analysis for adventitial agents and other safety concerns and thus is less hazardous than short-lived primary cell cultures for which testing must be repeated for each newly established culture.
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spelling pubmed-72711712020-06-05 Propagation of Adenoviral Vectors: Use of PER. C6 Cells Nichols, W.W. Lardenoije, R. Ledwith, B.J. Brouwer, K. Manam, S. Vogels, R. Kaslow, D. Zuidgeest, D. Bett, A.J. Chen, L. van der Kaaden, M. Galloway, S.M. Hill, R.B. Machotka, S.V. Anderson, C.A. Lewis, J. Martinez, D. Lebron, J. Russo, C. Valerio, D. Bout, A. Adenoviral Vectors for Gene Therapy Article This chapter discusses the production systems of adenoviral vectors. Particular attention is paid to the generation and use of complementation cell lines that carry the El genes and emphasizes on the PER.C6 cell line, which was developed to prevent generation of replication-competent adenovirus (RCA) during propagation of El-deleted adenoviral vectors. Further, safety issues with respect to the use of the cell line for making clinical grade material are also addressed in this chapter. The PER.C6 cell line is the best substrate for the production of adenoviral vectors for gene therapy or vaccines. PER.C6 permits extensive analysis for adventitial agents and other safety concerns and thus is less hazardous than short-lived primary cell cultures for which testing must be repeated for each newly established culture. 2002 2007-05-09 /pmc/articles/PMC7271171/ http://dx.doi.org/10.1016/B978-012199504-1/50007-9 Text en Copyright © 2002 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Nichols, W.W.
Lardenoije, R.
Ledwith, B.J.
Brouwer, K.
Manam, S.
Vogels, R.
Kaslow, D.
Zuidgeest, D.
Bett, A.J.
Chen, L.
van der Kaaden, M.
Galloway, S.M.
Hill, R.B.
Machotka, S.V.
Anderson, C.A.
Lewis, J.
Martinez, D.
Lebron, J.
Russo, C.
Valerio, D.
Bout, A.
Propagation of Adenoviral Vectors: Use of PER. C6 Cells
title Propagation of Adenoviral Vectors: Use of PER. C6 Cells
title_full Propagation of Adenoviral Vectors: Use of PER. C6 Cells
title_fullStr Propagation of Adenoviral Vectors: Use of PER. C6 Cells
title_full_unstemmed Propagation of Adenoviral Vectors: Use of PER. C6 Cells
title_short Propagation of Adenoviral Vectors: Use of PER. C6 Cells
title_sort propagation of adenoviral vectors: use of per. c6 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271171/
http://dx.doi.org/10.1016/B978-012199504-1/50007-9
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