Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes

OBJECTIVE: Vancomycin resistant enterococci (VRE) are of significant public health concern. The identification of VRE in livestock and food has increased. The objective of this study was to determine if the transferrable vancomycin resistance genes vanA and vanB were present in probiotics marketed f...

Descripción completa

Detalles Bibliográficos
Autores principales: Berreta, Ana, Baumgardner, Rachel M., Kopper, Jamie J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271421/
https://www.ncbi.nlm.nih.gov/pubmed/32498700
http://dx.doi.org/10.1186/s13104-020-05114-1
_version_ 1783542087267909632
author Berreta, Ana
Baumgardner, Rachel M.
Kopper, Jamie J.
author_facet Berreta, Ana
Baumgardner, Rachel M.
Kopper, Jamie J.
author_sort Berreta, Ana
collection PubMed
description OBJECTIVE: Vancomycin resistant enterococci (VRE) are of significant public health concern. The identification of VRE in livestock and food has increased. The objective of this study was to determine if the transferrable vancomycin resistance genes vanA and vanB were present in probiotics marketed for use in animals that claimed to contain Enterococcus spp. RESULTS: Of the 40 products selected, Enterococcus spp. DNA was successfully extracted from 36 products. Of these 36 products with enterococcal DNA, 2 (6%) had a PCR product consistent with vanA which was confirmed by sequencing. None of the products appeared to contain vanB.
format Online
Article
Text
id pubmed-7271421
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-72714212020-06-08 Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes Berreta, Ana Baumgardner, Rachel M. Kopper, Jamie J. BMC Res Notes Research Note OBJECTIVE: Vancomycin resistant enterococci (VRE) are of significant public health concern. The identification of VRE in livestock and food has increased. The objective of this study was to determine if the transferrable vancomycin resistance genes vanA and vanB were present in probiotics marketed for use in animals that claimed to contain Enterococcus spp. RESULTS: Of the 40 products selected, Enterococcus spp. DNA was successfully extracted from 36 products. Of these 36 products with enterococcal DNA, 2 (6%) had a PCR product consistent with vanA which was confirmed by sequencing. None of the products appeared to contain vanB. BioMed Central 2020-06-04 /pmc/articles/PMC7271421/ /pubmed/32498700 http://dx.doi.org/10.1186/s13104-020-05114-1 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Berreta, Ana
Baumgardner, Rachel M.
Kopper, Jamie J.
Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes
title Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes
title_full Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes
title_fullStr Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes
title_full_unstemmed Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes
title_short Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes
title_sort evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271421/
https://www.ncbi.nlm.nih.gov/pubmed/32498700
http://dx.doi.org/10.1186/s13104-020-05114-1
work_keys_str_mv AT berretaana evaluationofcommercialveterinaryprobioticscontainingenterococcifortransferrablevancomycinresistancegenes
AT baumgardnerrachelm evaluationofcommercialveterinaryprobioticscontainingenterococcifortransferrablevancomycinresistancegenes
AT kopperjamiej evaluationofcommercialveterinaryprobioticscontainingenterococcifortransferrablevancomycinresistancegenes

Ejemplares similares