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The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus
BACKGROUND: Limonoids are major bioactive compounds that are produced by the triterpenoid metabolic pathway. The detailed biochemical process of limonoid biosynthesis and the mechanism of its molecular regulation remain elusive. The identification of transcription factors that regulate limonoid bios...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271526/ https://www.ncbi.nlm.nih.gov/pubmed/32493275 http://dx.doi.org/10.1186/s12870-020-02475-4 |
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author | Zhang, Pan Liu, Xiaofeng Xu, Xin Wang, Fusheng Long, Junhong Shen, Wanxia Jiang, Dong Zhao, Xiaochun |
author_facet | Zhang, Pan Liu, Xiaofeng Xu, Xin Wang, Fusheng Long, Junhong Shen, Wanxia Jiang, Dong Zhao, Xiaochun |
author_sort | Zhang, Pan |
collection | PubMed |
description | BACKGROUND: Limonoids are major bioactive compounds that are produced by the triterpenoid metabolic pathway. The detailed biochemical process of limonoid biosynthesis and the mechanism of its molecular regulation remain elusive. The identification of transcription factors that regulate limonoid biosynthetic pathways is very important for understanding the underlying regulatory mechanisms. This information could also provide tools for manipulating biosynthesis genes to modulate limonoid production. RESULTS: In this study, the CiMYB42 transcription factor was isolated to identify its role in limonoid biosynthesis. Multiple alignment analysis and phylogenetic analysis demonstrated that CiMYB42 is a typical R2R3MYB transcription factor that shares high similarity of its amino acid sequence with AtMYB42. Limonoids contents were higher in Citrus sinensis and Citrus grandis than in other species. Limonoid accumulation during leaf development also showed diverse trends in different genotypes. The expression of CiMYB42 was significantly related to the limonoid content and the expression of CiOSC in some citrus accessions. The overexpression of CiMYB42 in sweet orange resulted in significant accumulation of limonin, whereas the downregulation of CiMYB42 by RNAi resulted in a dwarf phenotype and less nomilin accumulation. Furthermore, the results of a yeast one-hybrid assay and EMSA indicated that CiMYB42 binds exclusively to the TTGTTG sequence (type II MYB core) in the promoter of CiOSC. Together, these results suggest that CiMYB42 positively regulates limonoid biosynthesis by regulating the expression of CiOSC by binding to the TTGTTG sequence (type II MYB core) of its promoter. CONCLUSIONS: CiMYB42 is an important transcription activator involved in limonoid biosynthesis that regulates the expression of CiOSC by binding to the TTGTTG sequence (type II MYB core). |
format | Online Article Text |
id | pubmed-7271526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-72715262020-06-08 The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus Zhang, Pan Liu, Xiaofeng Xu, Xin Wang, Fusheng Long, Junhong Shen, Wanxia Jiang, Dong Zhao, Xiaochun BMC Plant Biol Research Article BACKGROUND: Limonoids are major bioactive compounds that are produced by the triterpenoid metabolic pathway. The detailed biochemical process of limonoid biosynthesis and the mechanism of its molecular regulation remain elusive. The identification of transcription factors that regulate limonoid biosynthetic pathways is very important for understanding the underlying regulatory mechanisms. This information could also provide tools for manipulating biosynthesis genes to modulate limonoid production. RESULTS: In this study, the CiMYB42 transcription factor was isolated to identify its role in limonoid biosynthesis. Multiple alignment analysis and phylogenetic analysis demonstrated that CiMYB42 is a typical R2R3MYB transcription factor that shares high similarity of its amino acid sequence with AtMYB42. Limonoids contents were higher in Citrus sinensis and Citrus grandis than in other species. Limonoid accumulation during leaf development also showed diverse trends in different genotypes. The expression of CiMYB42 was significantly related to the limonoid content and the expression of CiOSC in some citrus accessions. The overexpression of CiMYB42 in sweet orange resulted in significant accumulation of limonin, whereas the downregulation of CiMYB42 by RNAi resulted in a dwarf phenotype and less nomilin accumulation. Furthermore, the results of a yeast one-hybrid assay and EMSA indicated that CiMYB42 binds exclusively to the TTGTTG sequence (type II MYB core) in the promoter of CiOSC. Together, these results suggest that CiMYB42 positively regulates limonoid biosynthesis by regulating the expression of CiOSC by binding to the TTGTTG sequence (type II MYB core) of its promoter. CONCLUSIONS: CiMYB42 is an important transcription activator involved in limonoid biosynthesis that regulates the expression of CiOSC by binding to the TTGTTG sequence (type II MYB core). BioMed Central 2020-06-03 /pmc/articles/PMC7271526/ /pubmed/32493275 http://dx.doi.org/10.1186/s12870-020-02475-4 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Zhang, Pan Liu, Xiaofeng Xu, Xin Wang, Fusheng Long, Junhong Shen, Wanxia Jiang, Dong Zhao, Xiaochun The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus |
title | The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus |
title_full | The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus |
title_fullStr | The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus |
title_full_unstemmed | The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus |
title_short | The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus |
title_sort | myb transcription factor cimyb42 regulates limonoids biosynthesis in citrus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271526/ https://www.ncbi.nlm.nih.gov/pubmed/32493275 http://dx.doi.org/10.1186/s12870-020-02475-4 |
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