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Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway

Influence of lidocaine on rats with cerebral ischemia-reperfusion injury (CIRI) was studied to explore its mechanism of action. A total of 30 Sprague-Dawley rats were randomly divided into control group and model group, and the rat model of CIRI was prepared by the suture-occluded method in the mode...

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Autores principales: Liu, Yang, Zhang, Jie, Zan, Jingwei, Zhang, Fengxian, Liu, Guokai, Wu, Anshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271727/
https://www.ncbi.nlm.nih.gov/pubmed/32509019
http://dx.doi.org/10.3892/etm.2020.8688
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author Liu, Yang
Zhang, Jie
Zan, Jingwei
Zhang, Fengxian
Liu, Guokai
Wu, Anshi
author_facet Liu, Yang
Zhang, Jie
Zan, Jingwei
Zhang, Fengxian
Liu, Guokai
Wu, Anshi
author_sort Liu, Yang
collection PubMed
description Influence of lidocaine on rats with cerebral ischemia-reperfusion injury (CIRI) was studied to explore its mechanism of action. A total of 30 Sprague-Dawley rats were randomly divided into control group and model group, and the rat model of CIRI was prepared by the suture-occluded method in the model group. Then the rats in the model group were randomly assigned into the model group (n=10) and the lidocaine group (n=10). The neurological function score of rats was evaluated, and the levels of serum B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in rats were determined using ELISA. TUNEL assay was performed to detect the neuronal apoptosis in the brain of rats. The messenger ribonucleic acid (mRNA) and protein expression levels of cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) were measured via RT-PCR and western blotting, respectively. Compared with those in the control group, the rats in the model group had an elevated neurological function score, a raised level of Bcl-2, but a reduced level of Bax in the serum, an obviously increased rate of neuronal apoptosis in the brain and decreased mRNA and protein levels of cAMP and PKA in cerebral tissues. The rats in lidocaine group had a lower neurological function score, a lower level of Bcl-2, but a higher level of Bax in the serum, an evidently lower rate of neuronal apoptosis in the brain and higher mRNA and protein levels of cAMP and PKA in cerebral tissues than those in the model group. Lidocaine can improve the neurological function of rats with CIRI and inhibit neuronal apoptosis in the brain, and its mechanism of action may be related to the activation of the cAMP/PKA signaling pathway.
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spelling pubmed-72717272020-06-05 Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway Liu, Yang Zhang, Jie Zan, Jingwei Zhang, Fengxian Liu, Guokai Wu, Anshi Exp Ther Med Articles Influence of lidocaine on rats with cerebral ischemia-reperfusion injury (CIRI) was studied to explore its mechanism of action. A total of 30 Sprague-Dawley rats were randomly divided into control group and model group, and the rat model of CIRI was prepared by the suture-occluded method in the model group. Then the rats in the model group were randomly assigned into the model group (n=10) and the lidocaine group (n=10). The neurological function score of rats was evaluated, and the levels of serum B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in rats were determined using ELISA. TUNEL assay was performed to detect the neuronal apoptosis in the brain of rats. The messenger ribonucleic acid (mRNA) and protein expression levels of cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) were measured via RT-PCR and western blotting, respectively. Compared with those in the control group, the rats in the model group had an elevated neurological function score, a raised level of Bcl-2, but a reduced level of Bax in the serum, an obviously increased rate of neuronal apoptosis in the brain and decreased mRNA and protein levels of cAMP and PKA in cerebral tissues. The rats in lidocaine group had a lower neurological function score, a lower level of Bcl-2, but a higher level of Bax in the serum, an evidently lower rate of neuronal apoptosis in the brain and higher mRNA and protein levels of cAMP and PKA in cerebral tissues than those in the model group. Lidocaine can improve the neurological function of rats with CIRI and inhibit neuronal apoptosis in the brain, and its mechanism of action may be related to the activation of the cAMP/PKA signaling pathway. D.A. Spandidos 2020-07 2020-04-27 /pmc/articles/PMC7271727/ /pubmed/32509019 http://dx.doi.org/10.3892/etm.2020.8688 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Yang
Zhang, Jie
Zan, Jingwei
Zhang, Fengxian
Liu, Guokai
Wu, Anshi
Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway
title Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway
title_full Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway
title_fullStr Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway
title_full_unstemmed Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway
title_short Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway
title_sort lidocaine improves cerebral ischemia-reperfusion injury in rats through camp/pka signaling pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271727/
https://www.ncbi.nlm.nih.gov/pubmed/32509019
http://dx.doi.org/10.3892/etm.2020.8688
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