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Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas

Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause...

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Autores principales: Potts, Robert W.A., Gutierrez, Alejandro P., Cortés-Araya, Yennifer, Houston, Ross D., Bean, Tim P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271890/
https://www.ncbi.nlm.nih.gov/pubmed/32547861
http://dx.doi.org/10.7717/peerj.9180
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author Potts, Robert W.A.
Gutierrez, Alejandro P.
Cortés-Araya, Yennifer
Houston, Ross D.
Bean, Tim P.
author_facet Potts, Robert W.A.
Gutierrez, Alejandro P.
Cortés-Araya, Yennifer
Houston, Ross D.
Bean, Tim P.
author_sort Potts, Robert W.A.
collection PubMed
description Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture of primary cells from adult Pacific oyster tissues and identify novel cell morphologies that have not been reported previously. Cultures analysed by light microscopy, qPCR, and live cell imaging demonstrated maintenance of live, metabolically active Pacific oyster cells for several weeks post-explant. Interestingly, whole hearts dissected from adult oysters were found to continue contracting rhythmically up to 8 weeks after being transferred to a tissue culture system. Mantle tissue explants were also actively moving in the culture system. These improvements in primary cell culture of bivalves may be beneficial for research in ecotoxicology, virology, immunology, and genetic resistance to disease.
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spelling pubmed-72718902020-06-15 Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas Potts, Robert W.A. Gutierrez, Alejandro P. Cortés-Araya, Yennifer Houston, Ross D. Bean, Tim P. PeerJ Cell Biology Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture of primary cells from adult Pacific oyster tissues and identify novel cell morphologies that have not been reported previously. Cultures analysed by light microscopy, qPCR, and live cell imaging demonstrated maintenance of live, metabolically active Pacific oyster cells for several weeks post-explant. Interestingly, whole hearts dissected from adult oysters were found to continue contracting rhythmically up to 8 weeks after being transferred to a tissue culture system. Mantle tissue explants were also actively moving in the culture system. These improvements in primary cell culture of bivalves may be beneficial for research in ecotoxicology, virology, immunology, and genetic resistance to disease. PeerJ Inc. 2020-06-01 /pmc/articles/PMC7271890/ /pubmed/32547861 http://dx.doi.org/10.7717/peerj.9180 Text en ©2020 Potts et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Cell Biology
Potts, Robert W.A.
Gutierrez, Alejandro P.
Cortés-Araya, Yennifer
Houston, Ross D.
Bean, Tim P.
Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas
title Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas
title_full Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas
title_fullStr Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas
title_full_unstemmed Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas
title_short Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas
title_sort developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve crassostrea gigas
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271890/
https://www.ncbi.nlm.nih.gov/pubmed/32547861
http://dx.doi.org/10.7717/peerj.9180
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