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Effect of rotenone-induced stress on physiologically active substances in adult Aphis glycines

The aim of this study was to determine the effect of rotenone stress on Aphis glycines Matsumura (Hemiptera: Aphididae) populations in different habitats of Northeast China. The changes in kinase expression activity of endogenous substances (proteins, total sugars, trehalose, cholesterol, and free a...

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Autores principales: Han, Lanlan, Gao, Litong, Hao, Ziru, Zhao, Kuijun, Zhang, Wenlin, Chen, Juan, Xiao, Jianfei, Zhang, Aonan, Shi, Zhenghao, Zhu, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272001/
https://www.ncbi.nlm.nih.gov/pubmed/32497152
http://dx.doi.org/10.1371/journal.pone.0234137
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author Han, Lanlan
Gao, Litong
Hao, Ziru
Zhao, Kuijun
Zhang, Wenlin
Chen, Juan
Xiao, Jianfei
Zhang, Aonan
Shi, Zhenghao
Zhu, Lin
author_facet Han, Lanlan
Gao, Litong
Hao, Ziru
Zhao, Kuijun
Zhang, Wenlin
Chen, Juan
Xiao, Jianfei
Zhang, Aonan
Shi, Zhenghao
Zhu, Lin
author_sort Han, Lanlan
collection PubMed
description The aim of this study was to determine the effect of rotenone stress on Aphis glycines Matsumura (Hemiptera: Aphididae) populations in different habitats of Northeast China. The changes in kinase expression activity of endogenous substances (proteins, total sugars, trehalose, cholesterol, and free amino acids), detoxifying enzymes (cytochrome P450 and glutathione S-transferase), and metabolic enzymes (proteases and phosphofructokinases) in specimens from three populations were compared before and after stress with rotenone at median lethal concentration (LC(50)) and their response mechanisms were analyzed. Following a 24 h treatment with rotenone, the average LC(50) rotenone values in A. glycines specimens from field populations A and B, and a laboratory population were 4.39, 4.61, and 4.03 mg/L, respectively. The degree of changes in the kinase expression activity of endogenous substances also differed, which indicated a difference in the response of A. glycines specimens from varying habitats to LC(50) rotenone stress. The content of endogenous substances, detoxifying enzymes, and metabolic enzymes, except for that of free amino acids, changed significantly in all populations treated with rotenone at LC(50) compared with that in the control (P < 0.05). The decrease in protein and trehalose content, and the obstruction of cholesterol transportation owing to decreased feeding in stressed individuals were the causes of A. glycines death after rotenone treatment. Aphis glycines resistance to rotenone may be related to cytochrome P450 expression.
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spelling pubmed-72720012020-06-12 Effect of rotenone-induced stress on physiologically active substances in adult Aphis glycines Han, Lanlan Gao, Litong Hao, Ziru Zhao, Kuijun Zhang, Wenlin Chen, Juan Xiao, Jianfei Zhang, Aonan Shi, Zhenghao Zhu, Lin PLoS One Research Article The aim of this study was to determine the effect of rotenone stress on Aphis glycines Matsumura (Hemiptera: Aphididae) populations in different habitats of Northeast China. The changes in kinase expression activity of endogenous substances (proteins, total sugars, trehalose, cholesterol, and free amino acids), detoxifying enzymes (cytochrome P450 and glutathione S-transferase), and metabolic enzymes (proteases and phosphofructokinases) in specimens from three populations were compared before and after stress with rotenone at median lethal concentration (LC(50)) and their response mechanisms were analyzed. Following a 24 h treatment with rotenone, the average LC(50) rotenone values in A. glycines specimens from field populations A and B, and a laboratory population were 4.39, 4.61, and 4.03 mg/L, respectively. The degree of changes in the kinase expression activity of endogenous substances also differed, which indicated a difference in the response of A. glycines specimens from varying habitats to LC(50) rotenone stress. The content of endogenous substances, detoxifying enzymes, and metabolic enzymes, except for that of free amino acids, changed significantly in all populations treated with rotenone at LC(50) compared with that in the control (P < 0.05). The decrease in protein and trehalose content, and the obstruction of cholesterol transportation owing to decreased feeding in stressed individuals were the causes of A. glycines death after rotenone treatment. Aphis glycines resistance to rotenone may be related to cytochrome P450 expression. Public Library of Science 2020-06-04 /pmc/articles/PMC7272001/ /pubmed/32497152 http://dx.doi.org/10.1371/journal.pone.0234137 Text en © 2020 Han et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Han, Lanlan
Gao, Litong
Hao, Ziru
Zhao, Kuijun
Zhang, Wenlin
Chen, Juan
Xiao, Jianfei
Zhang, Aonan
Shi, Zhenghao
Zhu, Lin
Effect of rotenone-induced stress on physiologically active substances in adult Aphis glycines
title Effect of rotenone-induced stress on physiologically active substances in adult Aphis glycines
title_full Effect of rotenone-induced stress on physiologically active substances in adult Aphis glycines
title_fullStr Effect of rotenone-induced stress on physiologically active substances in adult Aphis glycines
title_full_unstemmed Effect of rotenone-induced stress on physiologically active substances in adult Aphis glycines
title_short Effect of rotenone-induced stress on physiologically active substances in adult Aphis glycines
title_sort effect of rotenone-induced stress on physiologically active substances in adult aphis glycines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272001/
https://www.ncbi.nlm.nih.gov/pubmed/32497152
http://dx.doi.org/10.1371/journal.pone.0234137
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