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Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity

Because avidin and biotin molecules exhibit the most specific and strongest non-covalent interaction, avidin-biotin technology is widely used in ELISA (enzyme-linked immunosorbent assay) kits for the detection of different bio-macromolecules linked to different diseases including cancer and influenz...

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Autores principales: Wang, Shiyu, Hossain, Md Zakir, Shinozuka, Kazuo, Shimizu, Natsuhiko, Kitada, Shunya, Suzuki, Takaaki, Ichige, Ryo, Kuwana, Anna, Kobayashi, Haruo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272179/
https://www.ncbi.nlm.nih.gov/pubmed/32729495
http://dx.doi.org/10.1016/j.bios.2020.112363
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author Wang, Shiyu
Hossain, Md Zakir
Shinozuka, Kazuo
Shimizu, Natsuhiko
Kitada, Shunya
Suzuki, Takaaki
Ichige, Ryo
Kuwana, Anna
Kobayashi, Haruo
author_facet Wang, Shiyu
Hossain, Md Zakir
Shinozuka, Kazuo
Shimizu, Natsuhiko
Kitada, Shunya
Suzuki, Takaaki
Ichige, Ryo
Kuwana, Anna
Kobayashi, Haruo
author_sort Wang, Shiyu
collection PubMed
description Because avidin and biotin molecules exhibit the most specific and strongest non-covalent interaction, avidin-biotin technology is widely used in ELISA (enzyme-linked immunosorbent assay) kits for the detection of different bio-macromolecules linked to different diseases including cancer and influenza. Combining the outstanding electrical conductivity (200,000 cm(2)V(-1)s(-1)) of graphene with the unique avidin and biotin interaction, we demonstrate a novel graphene field-effect transistor (GFET) biosensor for the quantitative detection of bio-macromolecules. The GFET consists of six pairs of interdigital Cr/Au electrodes supported on Si/SiO(2) substrate with an avidin immobilized single layer graphene channel as the sensing platform. By monitoring the real time current change upon the addition of biotin solution in bovine serum albumin (BSA) in the silicone pool preformed onto the GFET, the lowest detectable biotin concentration is estimated to be 90 fg/ml (0.37 pM). The specificity of the GFET is confirmed both by controlled and real sample measurements. From the magnitude of current change upon the addition of different concentrations of biotin solutions, the dissociation constant K(d) is estimated to be 1.6 × 10(-11) M. Since biotin is capable of conjugating with proteins, nucleotides and other bio-macromolecules without altering their properties, the present GFET sensor with its ultra-high sensitivity (0.37 pM) and specificity can be tailored to the rapid point-of-care detection of different types of desired biomolecules at very low concentration level through biotinylation as well as the exogenous biotin in blood serum.
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spelling pubmed-72721792020-06-05 Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity Wang, Shiyu Hossain, Md Zakir Shinozuka, Kazuo Shimizu, Natsuhiko Kitada, Shunya Suzuki, Takaaki Ichige, Ryo Kuwana, Anna Kobayashi, Haruo Biosens Bioelectron Article Because avidin and biotin molecules exhibit the most specific and strongest non-covalent interaction, avidin-biotin technology is widely used in ELISA (enzyme-linked immunosorbent assay) kits for the detection of different bio-macromolecules linked to different diseases including cancer and influenza. Combining the outstanding electrical conductivity (200,000 cm(2)V(-1)s(-1)) of graphene with the unique avidin and biotin interaction, we demonstrate a novel graphene field-effect transistor (GFET) biosensor for the quantitative detection of bio-macromolecules. The GFET consists of six pairs of interdigital Cr/Au electrodes supported on Si/SiO(2) substrate with an avidin immobilized single layer graphene channel as the sensing platform. By monitoring the real time current change upon the addition of biotin solution in bovine serum albumin (BSA) in the silicone pool preformed onto the GFET, the lowest detectable biotin concentration is estimated to be 90 fg/ml (0.37 pM). The specificity of the GFET is confirmed both by controlled and real sample measurements. From the magnitude of current change upon the addition of different concentrations of biotin solutions, the dissociation constant K(d) is estimated to be 1.6 × 10(-11) M. Since biotin is capable of conjugating with proteins, nucleotides and other bio-macromolecules without altering their properties, the present GFET sensor with its ultra-high sensitivity (0.37 pM) and specificity can be tailored to the rapid point-of-care detection of different types of desired biomolecules at very low concentration level through biotinylation as well as the exogenous biotin in blood serum. Elsevier B.V. 2020-10-01 2020-06-04 /pmc/articles/PMC7272179/ /pubmed/32729495 http://dx.doi.org/10.1016/j.bios.2020.112363 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Wang, Shiyu
Hossain, Md Zakir
Shinozuka, Kazuo
Shimizu, Natsuhiko
Kitada, Shunya
Suzuki, Takaaki
Ichige, Ryo
Kuwana, Anna
Kobayashi, Haruo
Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity
title Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity
title_full Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity
title_fullStr Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity
title_full_unstemmed Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity
title_short Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity
title_sort graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272179/
https://www.ncbi.nlm.nih.gov/pubmed/32729495
http://dx.doi.org/10.1016/j.bios.2020.112363
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