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V-ATPase subunit B plays essential roles in the molting process of the Chinese mitten crab, Eriocheir sinensis
Vacuolar ATPase (V-ATPase) is a proton pump driven by ATP hydrolysis, and it plays an important role in numerous biological processes, such as protein degradation and synthesis, cell growth, and cell autophagy. The V-ATPase subunit B (VATB) is a conservative and regulatory subunit required for ATP h...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272352/ https://www.ncbi.nlm.nih.gov/pubmed/32434771 http://dx.doi.org/10.1242/bio.048926 |
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author | Hou, Xin Chen, Xiaowen Yang, He Yue, Wucheng Wang, Jun Han, Hua Wang, Chenghui |
author_facet | Hou, Xin Chen, Xiaowen Yang, He Yue, Wucheng Wang, Jun Han, Hua Wang, Chenghui |
author_sort | Hou, Xin |
collection | PubMed |
description | Vacuolar ATPase (V-ATPase) is a proton pump driven by ATP hydrolysis, and it plays an important role in numerous biological processes, such as protein degradation and synthesis, cell growth, and cell autophagy. The V-ATPase subunit B (VATB) is a conservative and regulatory subunit required for ATP hydrolysis during proton pumping. The VATB of Eriocheir sinensis (EsVATB), which includes an open reading frame (ORF) length of 1467 bp encoding 489 amino acids, was cloned to unveil the biological function of VATB during the molting process of crustaceans. Spatial and temporal expression profiles showed that EsVATB was highly expressed in the posterior gill accompanied with the highest osmotic pressure in the premolt (PrM) stage. Meanwhile, the highest expression level of EsVATB was identified in the hepatopancreas and heart during the postmolt stage and epidermis in the intermolt stage, indicating that EsVATB may perform diverse biological functions in different tissues during the molting process. The individual crabs in the interference group showed a high mortality rate (74%) and a low molting rate (26%) and failed to form a new epicuticle in the PrM stage. Meanwhile, a significant difference in osmotic pressure was identified between the interference and control groups. Our results indicate that EsVATB is an indispensable functional gene that may participate in osmoregulation and help with the new epicuticle formation during the molting process of E. sinensis. |
format | Online Article Text |
id | pubmed-7272352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-72723522020-06-05 V-ATPase subunit B plays essential roles in the molting process of the Chinese mitten crab, Eriocheir sinensis Hou, Xin Chen, Xiaowen Yang, He Yue, Wucheng Wang, Jun Han, Hua Wang, Chenghui Biol Open Research Article Vacuolar ATPase (V-ATPase) is a proton pump driven by ATP hydrolysis, and it plays an important role in numerous biological processes, such as protein degradation and synthesis, cell growth, and cell autophagy. The V-ATPase subunit B (VATB) is a conservative and regulatory subunit required for ATP hydrolysis during proton pumping. The VATB of Eriocheir sinensis (EsVATB), which includes an open reading frame (ORF) length of 1467 bp encoding 489 amino acids, was cloned to unveil the biological function of VATB during the molting process of crustaceans. Spatial and temporal expression profiles showed that EsVATB was highly expressed in the posterior gill accompanied with the highest osmotic pressure in the premolt (PrM) stage. Meanwhile, the highest expression level of EsVATB was identified in the hepatopancreas and heart during the postmolt stage and epidermis in the intermolt stage, indicating that EsVATB may perform diverse biological functions in different tissues during the molting process. The individual crabs in the interference group showed a high mortality rate (74%) and a low molting rate (26%) and failed to form a new epicuticle in the PrM stage. Meanwhile, a significant difference in osmotic pressure was identified between the interference and control groups. Our results indicate that EsVATB is an indispensable functional gene that may participate in osmoregulation and help with the new epicuticle formation during the molting process of E. sinensis. The Company of Biologists Ltd 2020-05-29 /pmc/articles/PMC7272352/ /pubmed/32434771 http://dx.doi.org/10.1242/bio.048926 Text en © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Hou, Xin Chen, Xiaowen Yang, He Yue, Wucheng Wang, Jun Han, Hua Wang, Chenghui V-ATPase subunit B plays essential roles in the molting process of the Chinese mitten crab, Eriocheir sinensis |
title | V-ATPase subunit B plays essential roles in the molting process of the Chinese mitten crab, Eriocheir sinensis |
title_full | V-ATPase subunit B plays essential roles in the molting process of the Chinese mitten crab, Eriocheir sinensis |
title_fullStr | V-ATPase subunit B plays essential roles in the molting process of the Chinese mitten crab, Eriocheir sinensis |
title_full_unstemmed | V-ATPase subunit B plays essential roles in the molting process of the Chinese mitten crab, Eriocheir sinensis |
title_short | V-ATPase subunit B plays essential roles in the molting process of the Chinese mitten crab, Eriocheir sinensis |
title_sort | v-atpase subunit b plays essential roles in the molting process of the chinese mitten crab, eriocheir sinensis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272352/ https://www.ncbi.nlm.nih.gov/pubmed/32434771 http://dx.doi.org/10.1242/bio.048926 |
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