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Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers
We investigated the expression and subcellular localization of the SARS-CoV-2 receptor, angiotensin-converting enzyme 2 (ACE2), within the upper (nasal) and lower (pulmonary) respiratory tracts of healthy human donors. We detected ACE2 protein expression within the cilia organelle of ciliated airway...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273284/ https://www.ncbi.nlm.nih.gov/pubmed/32511516 http://dx.doi.org/10.1101/2020.05.08.20092866 |
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| author | Lee, Ivan T. Nakayama, Tsuguhisa Wu, Chien-Ting Goltsev, Yury Jiang, Sizun Gall, Phillip A. Liao, Chun-Kang Shih, Liang-Chun Schürch, Christian M. McIlwain, David R. Chu, Pauline Borchard, Nicole A. Zarabanda, David Dholakia, Sachi S. Yang, Angela Kim, Dayoung Kanie, Tomoharu Lin, Chia-Der Tsai, Ming-Hsui Phillips, Katie M. Kim, Raymond Overdevest, Jonathan B. Tyler, Matthew A. Yan, Carol H. Lin, Chih-Feng Lin, Yi-Tsen Bau, Da-Tian Tsay, Gregory J. Patel, Zara M. Tsou, Yung-An Tai, Chih-Jaan Yeh, Te-Huei Hwang, Peter H. Nolan, Garry P. Nayak, Jayakar V. Jackson, Peter K. |
| author_facet | Lee, Ivan T. Nakayama, Tsuguhisa Wu, Chien-Ting Goltsev, Yury Jiang, Sizun Gall, Phillip A. Liao, Chun-Kang Shih, Liang-Chun Schürch, Christian M. McIlwain, David R. Chu, Pauline Borchard, Nicole A. Zarabanda, David Dholakia, Sachi S. Yang, Angela Kim, Dayoung Kanie, Tomoharu Lin, Chia-Der Tsai, Ming-Hsui Phillips, Katie M. Kim, Raymond Overdevest, Jonathan B. Tyler, Matthew A. Yan, Carol H. Lin, Chih-Feng Lin, Yi-Tsen Bau, Da-Tian Tsay, Gregory J. Patel, Zara M. Tsou, Yung-An Tai, Chih-Jaan Yeh, Te-Huei Hwang, Peter H. Nolan, Garry P. Nayak, Jayakar V. Jackson, Peter K. |
| author_sort | Lee, Ivan T. |
| collection | PubMed |
| description | We investigated the expression and subcellular localization of the SARS-CoV-2 receptor, angiotensin-converting enzyme 2 (ACE2), within the upper (nasal) and lower (pulmonary) respiratory tracts of healthy human donors. We detected ACE2 protein expression within the cilia organelle of ciliated airway epithelial cells, which likely represents the initial or early subcellular site of SARS-CoV-2 viral entry during respiratory transmission. We further determined whether ACE2 expression in the cilia of upper respiratory cells was influenced by patient demographics, clinical characteristics, co-morbidities, or medication use, and found no evidence that the use of angiotensin-converting enzyme inhibitors (ACEI) or angiotensin II receptor blockers (ARBs) increases ACE2 protein expression. |
| format | Online Article Text |
| id | pubmed-7273284 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Cold Spring Harbor Laboratory |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-72732842020-06-07 Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers Lee, Ivan T. Nakayama, Tsuguhisa Wu, Chien-Ting Goltsev, Yury Jiang, Sizun Gall, Phillip A. Liao, Chun-Kang Shih, Liang-Chun Schürch, Christian M. McIlwain, David R. Chu, Pauline Borchard, Nicole A. Zarabanda, David Dholakia, Sachi S. Yang, Angela Kim, Dayoung Kanie, Tomoharu Lin, Chia-Der Tsai, Ming-Hsui Phillips, Katie M. Kim, Raymond Overdevest, Jonathan B. Tyler, Matthew A. Yan, Carol H. Lin, Chih-Feng Lin, Yi-Tsen Bau, Da-Tian Tsay, Gregory J. Patel, Zara M. Tsou, Yung-An Tai, Chih-Jaan Yeh, Te-Huei Hwang, Peter H. Nolan, Garry P. Nayak, Jayakar V. Jackson, Peter K. medRxiv Article We investigated the expression and subcellular localization of the SARS-CoV-2 receptor, angiotensin-converting enzyme 2 (ACE2), within the upper (nasal) and lower (pulmonary) respiratory tracts of healthy human donors. We detected ACE2 protein expression within the cilia organelle of ciliated airway epithelial cells, which likely represents the initial or early subcellular site of SARS-CoV-2 viral entry during respiratory transmission. We further determined whether ACE2 expression in the cilia of upper respiratory cells was influenced by patient demographics, clinical characteristics, co-morbidities, or medication use, and found no evidence that the use of angiotensin-converting enzyme inhibitors (ACEI) or angiotensin II receptor blockers (ARBs) increases ACE2 protein expression. Cold Spring Harbor Laboratory 2020-05-12 /pmc/articles/PMC7273284/ /pubmed/32511516 http://dx.doi.org/10.1101/2020.05.08.20092866 Text en https://creativecommons.org/licenses/by-nd/4.0/This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, and only so long as attribution is given to the creator. The license allows for commercial use. |
| spellingShingle | Article Lee, Ivan T. Nakayama, Tsuguhisa Wu, Chien-Ting Goltsev, Yury Jiang, Sizun Gall, Phillip A. Liao, Chun-Kang Shih, Liang-Chun Schürch, Christian M. McIlwain, David R. Chu, Pauline Borchard, Nicole A. Zarabanda, David Dholakia, Sachi S. Yang, Angela Kim, Dayoung Kanie, Tomoharu Lin, Chia-Der Tsai, Ming-Hsui Phillips, Katie M. Kim, Raymond Overdevest, Jonathan B. Tyler, Matthew A. Yan, Carol H. Lin, Chih-Feng Lin, Yi-Tsen Bau, Da-Tian Tsay, Gregory J. Patel, Zara M. Tsou, Yung-An Tai, Chih-Jaan Yeh, Te-Huei Hwang, Peter H. Nolan, Garry P. Nayak, Jayakar V. Jackson, Peter K. Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers |
| title | Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers |
| title_full | Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers |
| title_fullStr | Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers |
| title_full_unstemmed | Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers |
| title_short | Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers |
| title_sort | robust ace2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ace inhibitors or angiotensin receptor blockers |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273284/ https://www.ncbi.nlm.nih.gov/pubmed/32511516 http://dx.doi.org/10.1101/2020.05.08.20092866 |
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